//GC Dual Column Injection. Tray Name, Sample Number and Sample Volume are taken from the sample list. The second sample is taken from the same tray with "Second Sample Offset" added to the vial number. Time difference from first to second injection is set with "Injection Time Difference". To make this time accurate, the time needed to fill the syringe and move to the second injector has to be set in "Start Sampling to Injection Delay". Second Sample Offset ();0;0;2000 Air Volume (µl);0;0;SYR.Max Volume Pre Clean with Solvent 1 ();0;0;99 Pre Clean with Solvent 2 ();0;0;99 Pre Clean with Sample ();0;0;99 Filling Volume (µl);SYR.Fill Volume;SYR.Min Volume;SYR.Max Volume Filling Speed (µl/s);SYR.Fill Speed;SYR.Min Speed;SYR.Max Speed Filling Strokes ();1;0;99 Pullup Delay (ms);SYR.Pullup Del;0;10000 First Injection to;INJECTOR Second Injection to;INJECTOR Injection Time Difference (s);120;0;3600 Start Sampling to Injection Delay (s);60;0;3600 Injection Speed (µl/s);SYR.Inject Speed;SYR.Min Speed;SYR.Max Speed Pre Inject Delay (ms);500;0;99000 Post Inject Delay (ms);500;0;99000 Intermediate Clean w Solvent 1 ();3;0;99 Intermediate Clean w Solvent 2 ();0;0;99 Post Clean with Solvent 1 ();3;0;99 Post Clean with Solvent 2 ();0;0;99 LOCK_TERMINAL(On,) SET_OUT_SIG(Prep,On,,) SET_OUT_SIG(Prep,Off,,) WAIT_SYNC_SIG(Start,) CLEANUP(Wash1,Off,Off,On,Off,On,Off,Off,) CLEAN_SYR(Wash1,Pre Clean with Solvent 1,,,,,,,) CLEAN_SYR(Wash2,Pre Clean with Solvent 2,,,,,,,) REPEAT(Pre Clean with Sample,) GET_SAMPLE(SL.Tray,SL.Index,SL.Volume,Air Volume,,Filling Volume,Filling Speed,Pullup Delay,,0,Off,,,) PUT_SAMPLE(Waste,1,,,,,) END() GET_SAMPLE(SL.Tray,SL.Index,SL.Volume,Air Volume,,Filling Volume,Filling Speed,Pullup Delay,,Filling Strokes,Off,,,) INJ_SAMPLE(First Injection to,Inject,Injected,,,Pre Inject Delay,Injection Speed,Post Inject Delay,1,) SET_INJECTED() CLEAN_SYR(Wash1,Intermediate Clean w Solvent 1,,,,,,,) CLEAN_SYR(Wash2,Intermediate Clean w Solvent 2,,,,,,,) GET_SAMPLE(SL.Tray,SL.Index+Second Sample Offset,SL.Volume,Air Volume,,Filling Volume,Filling Speed,Pullup Delay,,Filling Strokes,Off,1,Injection Time Difference-Start Sampling to Injection Delay-5,) MOVETO_OBJECT(Second Injection to,1,0,) WAIT_TIMER(1,Injection Time Difference-1,) INJ_SAMPLE(Second Injection to,Inject2,Injectd2,,,Pre Inject Delay,Injection Speed,Post Inject Delay,,) CLEAN_SYR(Wash1,Post Clean with Solvent 1,,,,,,,) CLEAN_SYR(Wash2,Post Clean with Solvent 2,,,,,,,) CLEANUP(Wash1,Off,Off,Off,Off,Off,Off,On,) LOCK_TERMINAL(Off,) 06 LOCK_TERMINAL Terminal Locking On SET_OUT_SIG Output Signal Prep Signal State On Pulse Time(ms) SET_OUT_SIG Output Signal Prep Signal State Off Pulse Time(ms) WAIT_SYNC_SIG Sync Signal Start CLEANUP Wash Station Wash1 Reset Injectors Off Reset Temperatures Off Reset X Y Z On Reset Needle Guide Off Reset Syringe On Close Tray Off Go Home Off CLEAN_SYR Wash Station Wash1 Clean Cycles Pre Clean with Solvent 1 Clean Volume(%) Wash Penetration(mm) Fill Speed(µl/s) Pullup Delay(ms) Eject Speed(µl/s) Rinse Time(s) CLEAN_SYR Wash Station Wash2 Clean Cycles Pre Clean with Solvent 2 Clean Volume(%) Wash Penetration(mm) Fill Speed(µl/s) Pullup Delay(ms) Eject Speed(µl/s) Rinse Time(s) REPEAT Count Pre Clean with Sample GET_SAMPLE Source SL.Tray Index SL.Index Sample Volume(µl) SL.Volume Air Volume(µl) Air Volume Penetration(mm) Fill Volume(µl) Filling Volume Fill Speed(µl/s) Filling Speed Pullup Delay(ms) Pullup Delay Eject Speed(µl/s) Fill Strokes 0 Needle Blocking Off Wait for Timer Wait Time(s) PUT_SAMPLE Destination Waste Index 1 Penetration(mm) Volume(µl) Eject Speed(µl/s) Start Timer END GET_SAMPLE Source SL.Tray Index SL.Index Sample Volume(µl) SL.Volume Air Volume(µl) Air Volume Penetration(mm) Fill Volume(µl) Filling Volume Fill Speed(µl/s) Filling Speed Pullup Delay(ms) Pullup Delay Eject Speed(µl/s) Fill Strokes Filling Strokes Needle Blocking Off Wait for Timer Wait Time(s) INJ_SAMPLE Injector First Injection to Sync Signal Inject Injected Signal Injected Penetration(mm) Sample Volume(µl) Pre Inject Delay(ms) Pre Inject Delay Inject Speed(µl/s) Injection Speed Post Inject Delay(ms) Post Inject Delay Start Timer 1 SET_INJECTED CLEAN_SYR Wash Station Wash1 Clean Cycles Intermediate Clean w Solvent 1 Clean Volume(%) Wash Penetration(mm) Fill Speed(µl/s) Pullup Delay(ms) Eject Speed(µl/s) Rinse Time(s) CLEAN_SYR Wash Station Wash2 Clean Cycles Intermediate Clean w Solvent 2 Clean Volume(%) Wash Penetration(mm) Fill Speed(µl/s) Pullup Delay(ms) Eject Speed(µl/s) Rinse Time(s) GET_SAMPLE Source SL.Tray Index SL.Index+Second Sample Offset Sample Volume(µl) SL.Volume Air Volume(µl) Air Volume Penetration(mm) Fill Volume(µl) Filling Volume Fill Speed(µl/s) Filling Speed Pullup Delay(ms) Pullup Delay Eject Speed(µl/s) Fill Strokes Filling Strokes Needle Blocking Off Wait for Timer 1 Wait Time(s) Injection Time Difference-Start Sampling to Injection Delay-5 MOVETO_OBJECT Object Name Second Injection to Index 1 Penetration(mm) 0 WAIT_TIMER Timer 1 Time(s) Injection Time Difference-1 INJ_SAMPLE Injector Second Injection to Sync Signal Inject2 Injected Signal Injectd2 Penetration(mm) Sample Volume(µl) Pre Inject Delay(ms) Pre Inject Delay Inject Speed(µl/s) Injection Speed Post Inject Delay(ms) Post Inject Delay Start Timer CLEAN_SYR Wash Station Wash1 Clean Cycles Post Clean with Solvent 1 Clean Volume(%) Wash Penetration(mm) Fill Speed(µl/s) Pullup Delay(ms) Eject Speed(µl/s) Rinse Time(s) CLEAN_SYR Wash Station Wash2 Clean Cycles Post Clean with Solvent 2 Clean Volume(%) Wash Penetration(mm) Fill Speed(µl/s) Pullup Delay(ms) Eject Speed(µl/s) Rinse Time(s) CLEANUP Wash Station Wash1 Reset Injectors Off Reset Temperatures Off Reset X Y Z Off Reset Needle Guide Off Reset Syringe Off Close Tray Off Go Home On LOCK_TERMINAL Terminal Locking Off 03