//Headspace injection cycle. Tray Name, Sample Number and Sample Volume are taken from the sample list. Incubation Temperature (°C);30;30;200 Incubation Time (s);600;30;86400 Syringe Temperature (°C);50;30;150 Agitator Speed (rpm);500;250;750 Fill Speed (µl/s);SYR.Fill Speed;SYR.Min Speed;SYR.Max Speed Fill Strokes ();SYR.Fill Strokes;0;99 Pullup Delay (ms);SYR.Pullup Del;0;10000 Inject to;INJECTOR Injection Speed (µl/s);SYR.Inject Speed;SYR.Min Speed;SYR.Max Speed Pre Inject Delay (ms);500;0;99000 Post Inject Delay (ms);500;0;99000 Flush Time (s);10;0;600 GC Runtime (s);600;30;86400 LOCK_TERMINAL(On,) SET_TEMP(SYR,Syringe Temperature,0,) SET_TEMP(Agitator,Incubation Temperature,1,) SET_TEMP(SYR,Syringe Temperature,1,) TRANSP_VIAL(SL.Tray,SL.Index,Agitator,1,10,) MOVETO_OBJECT(Home,,,) SET_AGI(Agitator,On,Agitator Speed,,,,) WAIT_TIMER(10,Incubation Time,) SET_AGI(Agitator,Off,,,,,) WAIT_FOR_DS() WAIT_SYNC_SIG(Start,) GET_SAMPLE(Agitator,1,SL.Volume,0,,,Fill Speed,Pullup Delay,,Fill Strokes,Off,,,) INJ_SAMPLE(Inject to,Inject,Injected,,,Pre Inject Delay,Injection Speed,Pre Inject Delay,1,) START_TIMER(1,) TRANSP_VIAL(Agitator,1,SL.Tray,SL.Index,,) CLEANUP(Wash1,Off,Off,Off,Off,Off,Off,On,) START_FLUSH(Flush Time,) WAIT(Flush Time,) STOP_FLUSH() WAIT_TIMER_TOL(1,GC Runtime-Incubation Time+60,86399,) WAIT_TIMER_TOL(1,GC Runtime-Incubation Time+60,86399,) LOCK_TERMINAL(Off,) 06 LOCK_TERMINAL Terminal Locking On SET_TEMP Object Name SYR Temperature(°C) Syringe Temperature Accuracy +-(°C) 0 SET_TEMP Object Name Agitator Temperature(°C) Incubation Temperature Accuracy +-(°C) 1 SET_TEMP Object Name SYR Temperature(°C) Syringe Temperature Accuracy +-(°C) 1 TRANSP_VIAL From Tray SL.Tray From Index SL.Index To Tray Agitator To Index 1 Start Timer 10 MOVETO_OBJECT Object Name Home Index Penetration(mm) SET_AGI Agitator Agitator State On Speed(rpm) Agitator Speed On Time(s) Off Time(s) Start Timer WAIT_TIMER Timer 10 Time(s) Incubation Time SET_AGI Agitator Agitator State Off Speed(rpm) On Time(s) Off Time(s) Start Timer WAIT_FOR_DS WAIT_SYNC_SIG Sync Signal Start GET_SAMPLE Source Agitator Index 1 Sample Volume(µl) SL.Volume Air Volume(µl) 0 Penetration(mm) Fill Volume(µl) Fill Speed(µl/s) Fill Speed Pullup Delay(ms) Pullup Delay Eject Speed(µl/s) Fill Strokes Fill Strokes Needle Blocking Off Wait for Timer Wait Time(s) INJ_SAMPLE Injector Inject to Sync Signal Inject Injected Signal Injected Penetration(mm) Sample Volume(µl) Pre Inject Delay(ms) Pre Inject Delay Inject Speed(µl/s) Injection Speed Post Inject Delay(ms) Pre Inject Delay Start Timer 1 START_TIMER Timer 1 TRANSP_VIAL From Tray Agitator From Index 1 To Tray SL.Tray To Index SL.Index Start Timer CLEANUP Wash Station Wash1 Reset Injectors Off Reset Temperatures Off Reset X Y Z Off Reset Needle Guide Off Reset Syringe Off Close Tray Off Go Home On START_FLUSH Flush Time(s) Flush Time WAIT Time(s) Flush Time STOP_FLUSH WAIT_TIMER_TOL Timer 1 Time(s) GC Runtime-Incubation Time+60 Tolerance(s) 86399 WAIT_TIMER_TOL Timer 1 Time(s) GC Runtime-Incubation Time+60 Tolerance(s) 86399 LOCK_TERMINAL Terminal Locking Off 02