//--------------------------------------------------------------LC injection cycle for 4 channel system--------------------------------------------------------------Valco Valves are switched to the Load Position (A) by closing SW-Out1PAL waits for START to begin loading four independent valves with sample, cleaning in between. On completion the PAL waits for INJECT before sending a SW-Out2 signal to switch all valves to position B for 500ms, then switch back to A.The HPLC systems are triggered by signals from the valves.----------------------------------------Tray Names and Sample positions are passed as 'Tray1=xxx;Index1=n1;Tray2=yyy;Index2=n2;Tray3=zzz;Index3=n3;Tray4=aaa;Index3=n4;Volume=vvv' Pre Clean with Solvent 1 ();0;0;99 Pre Clean with Solvent 2 ();0;0;99 Filling Speed (µl/s);SYR.Fill Speed;SYR.Min Speed;SYR.Max Speed Filling Strokes ();1;0;99 Injection Speed (µl/s);SYR.Inject Speed;SYR.Min Speed;SYR.Max Speed Pullup Delay (ms);SYR.Pullup Del;0;99999 Post Clean with Solvent 1 ();1;0;99 Post Clean with Solvent 2 ();1;0;99 Valve Clean with Solvent 1 ();1;0;99 Valve Clean with Solvent 2 ();1;0;99 CLEANUP(Wash1,Off,Off,On,Off,On,Off,Off,) SWITCH_EVENT(SW-Out1,On,1000,) CLEAN_SYR(Wash1,Pre Clean with Solvent 1,,,,,,,) CLEAN_SYR(Wash2,Pre Clean with Solvent 2,,,,,,,) GET_SAMPLE(SL.Tray1,SL.Index1,SL.Volume,,,,Filling Speed,Pullup Delay,,Filling Strokes,Off,,,) INJ_SAMPLE(LC Vlv1,Inject2,CCRuning,,,,Injection Speed,,,) CLEAN_SYR(Wash1,Post Clean with Solvent 1,,,,,,,) CLEAN_SYR(Wash2,Post Clean with Solvent 2,,,,,,,) GET_SAMPLE(SL.Tray2,SL.Index2,SL.Volume,,,,Filling Speed,Pullup Delay,,Filling Strokes,Off,,,) INJ_SAMPLE(LC Vlv2,Inject2,CCRuning,,,,Injection Speed,,,) CLEAN_SYR(Wash1,Post Clean with Solvent 1,,,,,,,) CLEAN_SYR(Wash2,Post Clean with Solvent 2,,,,,,,) GET_SAMPLE(SL.Tray3,SL.Index3,SL.Volume,,,,Filling Speed,Pullup Delay,,Filling Strokes,Off,,,) INJ_SAMPLE(LC Vlv3,Inject2,CCRuning,,,,Injection Speed,,,) CLEAN_SYR(Wash1,Post Clean with Solvent 1,,,,,,,) CLEAN_SYR(Wash2,Post Clean with Solvent 2,,,,,,,) GET_SAMPLE(SL.Tray4,SL.Index4,SL.Volume,,,,Filling Speed,Pullup Delay,,Filling Strokes,Off,,,) INJ_SAMPLE(LC Vlv4,Inject2,CCRuning,,,,Injection Speed,,,) CLEAN_SYR(Wash1,Post Clean with Solvent 1,,,,,,,) CLEAN_SYR(Wash2,Post Clean with Solvent 2,,,,,,,) MOVETO_OBJECT(Home,1,,) WAIT_SYNC_SIG(Inject,) SWITCH_EVENT(SW-Out2,On,1000,) CLEAN_INJ(Wash1,LC Vlv1,Valve Clean with Solvent 1,,,,,,,,,) CLEAN_INJ(Wash2,LC Vlv1,Valve Clean with Solvent 2,,,,,,,,,) CLEAN_INJ(Wash1,LC Vlv2,Valve Clean with Solvent 1,,,,,,,,,) CLEAN_INJ(Wash2,LC Vlv2,Valve Clean with Solvent 2,,,,,,,,,) CLEAN_INJ(Wash1,LC Vlv3,Valve Clean with Solvent 1,,,,,,,,,) CLEAN_INJ(Wash2,LC Vlv3,Valve Clean with Solvent 2,,,,,,,,,) CLEAN_INJ(Wash1,LC Vlv4,Valve Clean with Solvent 1,,,,,,,,,) CLEAN_INJ(Wash2,LC Vlv4,Valve Clean with Solvent 2,,,,,,,,,) CLEANUP(Wash1,Off,Off,On,Off,On,On,On,) 06 CLEANUP Wash Station Wash1 Reset Injectors Off Reset Temperatures Off Reset X Y Z On Reset Needle Guide Off Reset Syringe On Close Tray Off Go Home Off SWITCH_EVENT Event SW-Out1 Signal State On Pulse Time(ms) 1000 CLEAN_SYR Wash Station Wash1 Clean Cycles Pre Clean with Solvent 1 Clean Volume(%) Wash Penetration(mm) Fill Speed(µl/s) Pullup Delay(ms) Eject Speed(µl/s) Rinse Time(s) CLEAN_SYR Wash Station Wash2 Clean Cycles Pre Clean with Solvent 2 Clean Volume(%) Wash Penetration(mm) Fill Speed(µl/s) Pullup Delay(ms) Eject Speed(µl/s) Rinse Time(s) GET_SAMPLE Source SL.Tray1 Index SL.Index1 Sample Volume(µl) SL.Volume Air Volume(µl) Penetration(mm) Fill Volume(µl) Fill Speed(µl/s) Filling Speed Pullup Delay(ms) Pullup Delay Eject Speed(µl/s) Fill Strokes Filling Strokes Needle Blocking Off Wait for Timer Wait Time(s) INJ_SAMPLE Injector LC Vlv1 Sync Signal Inject2 Injected Signal CCRuning Penetration(mm) Sample Volume(µl) Pre Inject Delay(ms) Inject Speed(µl/s) Injection Speed Post Inject Delay(ms) Start Timer CLEAN_SYR Wash Station Wash1 Clean Cycles Post Clean with Solvent 1 Clean Volume(%) Wash Penetration(mm) Fill Speed(µl/s) Pullup Delay(ms) Eject Speed(µl/s) Rinse Time(s) CLEAN_SYR Wash Station Wash2 Clean Cycles Post Clean with Solvent 2 Clean Volume(%) Wash Penetration(mm) Fill Speed(µl/s) Pullup Delay(ms) Eject Speed(µl/s) Rinse Time(s) GET_SAMPLE Source SL.Tray2 Index SL.Index2 Sample Volume(µl) SL.Volume Air Volume(µl) Penetration(mm) Fill Volume(µl) Fill Speed(µl/s) Filling Speed Pullup Delay(ms) Pullup Delay Eject Speed(µl/s) Fill Strokes Filling Strokes Needle Blocking Off Wait for Timer Wait Time(s) INJ_SAMPLE Injector LC Vlv2 Sync Signal Inject2 Injected Signal CCRuning Penetration(mm) Sample Volume(µl) Pre Inject Delay(ms) Inject Speed(µl/s) Injection Speed Post Inject Delay(ms) Start Timer CLEAN_SYR Wash Station Wash1 Clean Cycles Post Clean with Solvent 1 Clean Volume(%) Wash Penetration(mm) Fill Speed(µl/s) Pullup Delay(ms) Eject Speed(µl/s) Rinse Time(s) CLEAN_SYR Wash Station Wash2 Clean Cycles Post Clean with Solvent 2 Clean Volume(%) Wash Penetration(mm) Fill Speed(µl/s) Pullup Delay(ms) Eject Speed(µl/s) Rinse Time(s) GET_SAMPLE Source SL.Tray3 Index SL.Index3 Sample Volume(µl) SL.Volume Air Volume(µl) Penetration(mm) Fill Volume(µl) Fill Speed(µl/s) Filling Speed Pullup Delay(ms) Pullup Delay Eject Speed(µl/s) Fill Strokes Filling Strokes Needle Blocking Off Wait for Timer Wait Time(s) INJ_SAMPLE Injector LC Vlv3 Sync Signal Inject2 Injected Signal CCRuning Penetration(mm) Sample Volume(µl) Pre Inject Delay(ms) Inject Speed(µl/s) Injection Speed Post Inject Delay(ms) Start Timer CLEAN_SYR Wash Station Wash1 Clean Cycles Post Clean with Solvent 1 Clean Volume(%) Wash Penetration(mm) Fill Speed(µl/s) Pullup Delay(ms) Eject Speed(µl/s) Rinse Time(s) CLEAN_SYR Wash Station Wash2 Clean Cycles Post Clean with Solvent 2 Clean Volume(%) Wash Penetration(mm) Fill Speed(µl/s) Pullup Delay(ms) Eject Speed(µl/s) Rinse Time(s) GET_SAMPLE Source SL.Tray4 Index SL.Index4 Sample Volume(µl) SL.Volume Air Volume(µl) Penetration(mm) Fill Volume(µl) Fill Speed(µl/s) Filling Speed Pullup Delay(ms) Pullup Delay Eject Speed(µl/s) Fill Strokes Filling Strokes Needle Blocking Off Wait for Timer Wait Time(s) INJ_SAMPLE Injector LC Vlv4 Sync Signal Inject2 Injected Signal CCRuning Penetration(mm) Sample Volume(µl) Pre Inject Delay(ms) Inject Speed(µl/s) Injection Speed Post Inject Delay(ms) Start Timer CLEAN_SYR Wash Station Wash1 Clean Cycles Post Clean with Solvent 1 Clean Volume(%) Wash Penetration(mm) Fill Speed(µl/s) Pullup Delay(ms) Eject Speed(µl/s) Rinse Time(s) CLEAN_SYR Wash Station Wash2 Clean Cycles Post Clean with Solvent 2 Clean Volume(%) Wash Penetration(mm) Fill Speed(µl/s) Pullup Delay(ms) Eject Speed(µl/s) Rinse Time(s) MOVETO_OBJECT Object Name Home Index 1 Penetration(mm) WAIT_SYNC_SIG Sync Signal Inject SWITCH_EVENT Event SW-Out2 Signal State On Pulse Time(ms) 1000 CLEAN_INJ Wash Station Wash1 Injector LC Vlv1 Clean Cycles Valve Clean with Solvent 1 Clean Volume(%) Wash Penetration(mm) Fill Speed(µl/s) Pullup Delay(ms) Eject Speed(µl/s) Rinse Time(s) Inject Penetration(mm) Injection Speed(µl/s) CLEAN_INJ Wash Station Wash2 Injector LC Vlv1 Clean Cycles Valve Clean with Solvent 2 Clean Volume(%) Wash Penetration(mm) Fill Speed(µl/s) Pullup Delay(ms) Eject Speed(µl/s) Rinse Time(s) Inject Penetration(mm) Injection Speed(µl/s) CLEAN_INJ Wash Station Wash1 Injector LC Vlv2 Clean Cycles Valve Clean with Solvent 1 Clean Volume(%) Wash Penetration(mm) Fill Speed(µl/s) Pullup Delay(ms) Eject Speed(µl/s) Rinse Time(s) Inject Penetration(mm) Injection Speed(µl/s) CLEAN_INJ Wash Station Wash2 Injector LC Vlv2 Clean Cycles Valve Clean with Solvent 2 Clean Volume(%) Wash Penetration(mm) Fill Speed(µl/s) Pullup Delay(ms) Eject Speed(µl/s) Rinse Time(s) Inject Penetration(mm) Injection Speed(µl/s) CLEAN_INJ Wash Station Wash1 Injector LC Vlv3 Clean Cycles Valve Clean with Solvent 1 Clean Volume(%) Wash Penetration(mm) Fill Speed(µl/s) Pullup Delay(ms) Eject Speed(µl/s) Rinse Time(s) Inject Penetration(mm) Injection Speed(µl/s) CLEAN_INJ Wash Station Wash2 Injector LC Vlv3 Clean Cycles Valve Clean with Solvent 2 Clean Volume(%) Wash Penetration(mm) Fill Speed(µl/s) Pullup Delay(ms) Eject Speed(µl/s) Rinse Time(s) Inject Penetration(mm) Injection Speed(µl/s) CLEAN_INJ Wash Station Wash1 Injector LC Vlv4 Clean Cycles Valve Clean with Solvent 1 Clean Volume(%) Wash Penetration(mm) Fill Speed(µl/s) Pullup Delay(ms) Eject Speed(µl/s) Rinse Time(s) Inject Penetration(mm) Injection Speed(µl/s) CLEAN_INJ Wash Station Wash2 Injector LC Vlv4 Clean Cycles Valve Clean with Solvent 2 Clean Volume(%) Wash Penetration(mm) Fill Speed(µl/s) Pullup Delay(ms) Eject Speed(µl/s) Rinse Time(s) Inject Penetration(mm) Injection Speed(µl/s) CLEANUP Wash Station Wash1 Reset Injectors Off Reset Temperatures Off Reset X Y Z On Reset Needle Guide Off Reset Syringe On Close Tray On Go Home On 02