//Cycle for SPME with Agitation and Heating 1. Wait for temperature of Agitator 2. Move vial to Agitator 3. Heat and agitate during "Pre Incubation Time" 4. Insert needle into vial in Agitator 5. Expose fiber 6. Extract during "Extraction Time" 7. Wait for "Start" signal 8. Insert needle into injector 9. Expose fiber 10. Activate "Injected" signal 11. Desorb for "Desorption Time" 12. Remove vial from Agitator 13. Wait for GC Runtime - Pre Incubation Time - Extraction Time (Wait until next vial can be inserted into Agitator) Pre Incubation Time (s);60;10;6000 Incubation Temp. (°C);50;30;200 Pre Inc Agitator Speed (rpm);500;250;750 Agitator On Time (s);5;0;60 Agitator Off Time (s);2;0;60 Vial Penetration in mm (µl);22;22;31 Extraction Time (s);60;10;6000 Desorb to;INJECTOR Injection Penetration in mm (µl);54;44;57 Desorption Time (s);60;10;86400 GC Runtime (s);300;60;86400 LOCK_TERMINAL(On,) SET_TEMP(Agitator,Incubation Temp.,1,) TRANSP_VIAL(SL.Tray,SL.Index,Agitator,1,,) MOVETO_OBJECT(Home,,,) SET_AGI(Agitator,On,Pre Inc Agitator Speed,Agitator On Time,Agitator Off Time,1,) WAIT_TIMER(1,Pre Incubation Time,) SET_AGI(Agitator,Off,,,,,) MOVETO_OBJECT(FiberExp,,25,) BLOCK_NDLG(On,Off,) MOVETO_OBJECT(Agitator,1,11,) MOVE_REL(,2.5,,) DISPENSE_SYR(Vial Penetration in mm-10,,) SET_AGI(Agitator,On,250,Agitator On Time,0,2,) WAIT_TIMER(2,Extraction Time,) SET_AGI(Agitator,Off,,,,,) ASPIRATE_SYR(Vial Penetration in mm-10,,,,) MOVE_REL(,-2.5,,) WAIT_FOR_DS() WAIT_SYNC_SIG(Start,) BLOCK_NDLG(Off,On,) MOVETO_OBJECT(Desorb to,,33,) DISPENSE_SYR(Injection Penetration in mm-32,,) SET_OUT_SIG(Injected,On,,) START_TIMER(1,) WAIT_TIMER(1,Desorption Time,) ASPIRATE_SYR(Injection Penetration in mm-32,,,,) TRANSP_VIAL(Agitator,1,SL.Tray,SL.Index,0,) MOVETO_OBJECT(Home,,,) WAIT_TIMER_TOL(1,GC Runtime-Pre Incubation Time-Extraction Time-20,86399,) LOCK_TERMINAL(Off,) 06 LOCK_TERMINAL Terminal Locking On SET_TEMP Object Name Agitator Temperature(°C) Incubation Temp. Accuracy +-(°C) 1 TRANSP_VIAL From Tray SL.Tray From Index SL.Index To Tray Agitator To Index 1 Start Timer MOVETO_OBJECT Object Name Home Index Penetration(mm) SET_AGI Agitator Agitator State On Speed(rpm) Pre Inc Agitator Speed On Time(s) Agitator On Time Off Time(s) Agitator Off Time Start Timer 1 WAIT_TIMER Timer 1 Time(s) Pre Incubation Time SET_AGI Agitator Agitator State Off Speed(rpm) On Time(s) Off Time(s) Start Timer MOVETO_OBJECT Object Name FiberExp Index Penetration(mm) 25 BLOCK_NDLG Block NdlGuide On Check Released Off MOVETO_OBJECT Object Name Agitator Index 1 Penetration(mm) 11 MOVE_REL RelX(mm) RelY(mm) 2.5 RelZ(mm) DISPENSE_SYR Volume(µl) Vial Penetration in mm-10 Eject Speed(µl/s) SET_AGI Agitator Agitator State On Speed(rpm) 250 On Time(s) Agitator On Time Off Time(s) 0 Start Timer 2 WAIT_TIMER Timer 2 Time(s) Extraction Time SET_AGI Agitator Agitator State Off Speed(rpm) On Time(s) Off Time(s) Start Timer ASPIRATE_SYR Volume(µl) Vial Penetration in mm-10 Overfill Rate(%) Fill Speed(µl/s) Pullup Delay(ms) MOVE_REL RelX(mm) RelY(mm) -2.5 RelZ(mm) WAIT_FOR_DS WAIT_SYNC_SIG Sync Signal Start BLOCK_NDLG Block NdlGuide Off Check Released On MOVETO_OBJECT Object Name Desorb to Index Penetration(mm) 33 DISPENSE_SYR Volume(µl) Injection Penetration in mm-32 Eject Speed(µl/s) SET_OUT_SIG Output Signal Injected Signal State On Pulse Time(ms) START_TIMER Timer 1 WAIT_TIMER Timer 1 Time(s) Desorption Time ASPIRATE_SYR Volume(µl) Injection Penetration in mm-32 Overfill Rate(%) Fill Speed(µl/s) Pullup Delay(ms) TRANSP_VIAL From Tray Agitator From Index 1 To Tray SL.Tray To Index SL.Index Start Timer 0 MOVETO_OBJECT Object Name Home Index Penetration(mm) WAIT_TIMER_TOL Timer 1 Time(s) GC Runtime-Pre Incubation Time-Extraction Time-20 Tolerance(s) 86399 LOCK_TERMINAL Terminal Locking Off 02