﻿<?xml version="1.0" encoding="utf-8"?>
<srm_settings format_version="19.1" software_version="Skyline (64-bit) 19.1.0.193">
  <settings_summary name="Default">
    <peptide_settings>
      <enzyme name="Trypsin" cut="KR" no_cut="P" sense="C" />
      <digest_settings max_missed_cleavages="2" />
      <peptide_prediction use_measured_rts="false" measured_rt_window="3" use_spectral_library_drift_times="false" spectral_library_drift_times_peak_width_calc_type="resolving_power" spectral_library_drift_times_resolving_power="0" spectral_library_drift_times_width_at_dt_zero="0" spectral_library_drift_times_width_at_dt_max="0">
        <predict_retention_time name="PPRT" time_window="10">
          <irt_calculator name="PPRT" database_path="D:\Skyline files\PPRT retention time calibration.irtdb" />
        </predict_retention_time>
      </peptide_prediction>
      <peptide_filter start="25" min_length="8" max_length="25" auto_select="true">
        <peptide_exclusions />
      </peptide_filter>
      <peptide_libraries pick="library" />
      <peptide_modifications max_variable_mods="3" max_neutral_losses="1" internal_standard="none">
        <static_modifications>
          <static_modification name="Carbamidomethyl (C)" aminoacid="C" formula="H3C2NO" unimod_id="4" short_name="CAM" />
          <static_modification name="Deamidated (NQ)" aminoacid="N, Q" variable="true" formula="O - HN" unimod_id="7" short_name="Dea" />
          <static_modification name="Oxidation (M)" aminoacid="M" variable="true" formula="O" unimod_id="35" short_name="Oxi">
            <potential_loss formula="H4COS" massdiff_monoisotopic="63.998285" massdiff_average="64.10701" />
          </static_modification>
        </static_modifications>
        <heavy_modifications>
          <static_modification name="Label:13C(6)15N(2) (C-term K)" aminoacid="K" terminus="C" label_13C="true" label_15N="true" />
          <static_modification name="Label:13C(6)15N(4) (C-term R)" aminoacid="R" terminus="C" label_13C="true" label_15N="true" />
        </heavy_modifications>
      </peptide_modifications>
      <quantification weighting="none" fit="none" normalization="none" ms_level="1" units="pmol" />
    </peptide_settings>
    <transition_settings>
      <transition_prediction precursor_mass_type="Monoisotopic" fragment_mass_type="Monoisotopic" optimize_by="None">
        <predict_collision_energy name="Thermo TSQ Ultra" step_size="1" step_count="5">
          <regression_ce charge="2" slope="0.036" intercept="0.954" />
          <regression_ce charge="3" slope="0.037" intercept="3.525" />
        </predict_collision_energy>
      </transition_prediction>
      <transition_filter precursor_charges="2" product_charges="1" precursor_adducts="[M-3H],[M-2H],[M-H],[M-],[M+H],[M+],[M+2H],[M+3H]" product_adducts="[M-3],[M-2],[M-],[M+],[M+2],[M+3]" fragment_types="p" small_molecule_fragment_types="f" fragment_range_first="(m/z &gt; precursor) - 2" fragment_range_last="last ion - 2" precursor_mz_window="0" auto_select="true">
        <measured_ion name="TMT-131" ion_formula="C4C'4H16N'" mass_monoisotopic="131.1387288096" mass_average="131.1839682496" charge="1" optional="true" />
      </transition_filter>
      <transition_libraries ion_match_tolerance="0.2" min_ion_count="0" ion_count="6" pick_from="none" />
      <transition_integration />
      <transition_instrument min_mz="300" max_mz="1600" mz_match_tolerance="0.5" min_time="15" max_time="70" />
      <transition_full_scan precursor_isotopes="Count" precursor_isotope_filter="4" precursor_mass_analyzer="orbitrap" precursor_res="60000" precursor_res_mz="200" selective_extraction="true" retention_time_filter_type="scheduling_windows" retention_time_filter_length="3">
        <isotope_enrichments name="Default">
          <atom_percent_enrichment symbol="H'">0.98</atom_percent_enrichment>
          <atom_percent_enrichment symbol="C'">0.995</atom_percent_enrichment>
          <atom_percent_enrichment symbol="N'">0.995</atom_percent_enrichment>
          <atom_percent_enrichment symbol="O&quot;">0.99</atom_percent_enrichment>
          <atom_percent_enrichment symbol="O'">0.99</atom_percent_enrichment>
        </isotope_enrichments>
      </transition_full_scan>
    </transition_settings>
    <data_settings document_guid="c7418b8c-4ea5-4739-a378-317f58de4558">
      <annotation name="Hela digest (ug)" targets="replicate" type="value_list">
        <value>1</value>
        <value>2</value>
      </annotation>
      <annotation name="PPRT (fmol)" targets="replicate" type="value_list">
        <value>250</value>
        <value>500</value>
        <value>1000</value>
      </annotation>
    </data_settings>
    <measured_results time_normal_area="true">
      <replicate name="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1">
        <sample_file id="_20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1_f0" file_path="D:\MS_DATA\DATA\20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1.raw" sample_name="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" acquired_time="2019-01-29T02:04:21" modified_time="2019-01-29T03:19:23" tic_area="825626329088">
          <instrument_info_list>
            <instrument_info>
              <model>Orbitrap Fusion</model>
              <ionsource>nanoelectrospray</ionsource>
              <analyzer>quadrupole/orbitrap</analyzer>
              <detector>inductive detector</detector>
            </instrument_info>
            <instrument_info>
              <model>Orbitrap Fusion</model>
              <ionsource>nanoelectrospray</ionsource>
              <analyzer>quadrupole/radial ejection linear ion trap</analyzer>
              <detector>electron multiplier</detector>
            </instrument_info>
          </instrument_info_list>
        </sample_file>
      </replicate>
      <replicate name="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2">
        <sample_file id="_20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2_f0" file_path="D:\MS_DATA\DATA\20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2.raw" sample_name="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" acquired_time="2019-01-29T03:51:20" modified_time="2019-01-29T05:06:23" tic_area="851676561408">
          <instrument_info_list>
            <instrument_info>
              <model>Orbitrap Fusion</model>
              <ionsource>nanoelectrospray</ionsource>
              <analyzer>quadrupole/orbitrap</analyzer>
              <detector>inductive detector</detector>
            </instrument_info>
            <instrument_info>
              <model>Orbitrap Fusion</model>
              <ionsource>nanoelectrospray</ionsource>
              <analyzer>quadrupole/radial ejection linear ion trap</analyzer>
              <detector>electron multiplier</detector>
            </instrument_info>
          </instrument_info_list>
        </sample_file>
      </replicate>
      <replicate name="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1">
        <sample_file id="_20191029_RS_Hela_QC_1ug_Tr_High_11ms_1_f0" file_path="D:\QC SKyline\20191029_RS_Hela_QC_1ug_Tr_High_11ms_1.raw" sample_name="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" acquired_time="2019-01-29T17:52:10" modified_time="2019-01-29T19:07:17" tic_area="1702413467648">
          <instrument_info_list>
            <instrument_info>
              <model>Q Exactive</model>
              <ionsource>nanoelectrospray</ionsource>
              <analyzer>quadrupole/orbitrap</analyzer>
              <detector>inductive detector</detector>
            </instrument_info>
          </instrument_info_list>
        </sample_file>
      </replicate>
      <replicate name="20190130_RS_Hela_QC_1">
        <sample_file id="_20190130_RS_Hela_QC_1_f0" file_path="D:\QC SKyline\20190130_RS_Hela_QC_1.raw" sample_name="20190130_RS_Hela_QC_1" acquired_time="2019-01-30T17:00:27" modified_time="2019-01-30T18:15:34" tic_area="327749042176">
          <instrument_info_list>
            <instrument_info>
              <model>Q Exactive</model>
              <ionsource>nanoelectrospray</ionsource>
              <analyzer>quadrupole/orbitrap</analyzer>
              <detector>inductive detector</detector>
            </instrument_info>
          </instrument_info_list>
        </sample_file>
      </replicate>
      <replicate name="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)">
        <sample_file id="_20190725_FPL_Hela_1ug_PPRT_500fm_QC_1_:Fusion_ES803A:_f0" file_path="D:\Skyline files\20190725_FPL_Hela_1ug_PPRT_500fm_QC_1.raw" sample_name="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1" acquired_time="2019-07-25T16:34:21" modified_time="2019-07-25T17:49:23" tic_area="1035101405184" ion_mobility_type="compensation_V">
          <instrument_info_list>
            <instrument_info>
              <model>Orbitrap Fusion</model>
              <ionsource>nanoelectrospray</ionsource>
              <analyzer>quadrupole/orbitrap</analyzer>
              <detector>inductive detector</detector>
            </instrument_info>
            <instrument_info>
              <model>Orbitrap Fusion</model>
              <ionsource>nanoelectrospray</ionsource>
              <analyzer>quadrupole/radial ejection linear ion trap</analyzer>
              <detector>electron multiplier</detector>
            </instrument_info>
          </instrument_info_list>
        </sample_file>
      </replicate>
      <replicate name="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)">
        <sample_file id="_20190731_HeLa_1ug_PPRT_500fmol_:SP25_column:_f0" file_path="D:\Skyline files\20190731_HeLa_1ug_PPRT_500fmol.raw" sample_name="20190731_HeLa_1ug_PPRT_500fmol" acquired_time="2019-07-31T18:09:29" modified_time="2019-07-31T19:24:33" tic_area="2399702614016" ion_mobility_type="compensation_V">
          <instrument_info_list>
            <instrument_info>
              <model>Q Exactive</model>
              <ionsource>nanoelectrospray</ionsource>
              <analyzer>quadrupole/orbitrap</analyzer>
              <detector>inductive detector</detector>
            </instrument_info>
          </instrument_info_list>
        </sample_file>
      </replicate>
      <replicate name="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)">
        <sample_file id="_20190731_HeLa_1ug_PPRT_500fmol_20190801190736_:SP25_new:_f0" file_path="D:\Skyline files\20190731_HeLa_1ug_PPRT_500fmol_20190801190736.raw" sample_name="20190731_HeLa_1ug_PPRT_500fmol_20190801190736" acquired_time="2019-08-01T19:32:29" modified_time="2019-08-01T20:47:35" tic_area="2326154706944" ion_mobility_type="compensation_V">
          <instrument_info_list>
            <instrument_info>
              <model>Q Exactive</model>
              <ionsource>nanoelectrospray</ionsource>
              <analyzer>quadrupole/orbitrap</analyzer>
              <detector>inductive detector</detector>
            </instrument_info>
          </instrument_info_list>
        </sample_file>
      </replicate>
    </measured_results>
  </settings_summary>
  <peptide_list label_name="standards" websearch_status="X#Upeptides1" auto_manage_children="false">
    <peptide auto_manage_children="false" standard_type="iRT" sequence="SSAAPPPPPR" modified_sequence="SSAAPPPPPR" calc_neutral_pep_mass="975.51378" num_missed_cleavages="0" rt_calculator_score="0" predicted_retention_time="19.72" avg_measured_retention_time="19.9336853">
      <implicit_modifications>
        <implicit_heavy_modifications>
          <implicit_modification index_aa="9" modification_name="Label:13C(6)15N(4) (C-term R)" mass_diff="+10" />
        </implicit_heavy_modifications>
      </implicit_modifications>
      <peptide_results>
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="18.6450176" predicted_retention_time="18.16" />
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="18.4602032" predicted_retention_time="18.02" />
        <peptide_result replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="19.7548676" predicted_retention_time="19.14" />
        <peptide_result replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="20.726902" predicted_retention_time="20.44" />
        <peptide_result replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="19.8964272" predicted_retention_time="19.19" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="20.9372749" predicted_retention_time="21.48" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="21.1151047" predicted_retention_time="21.65" />
      </peptide_results>
      <precursor charge="2" isotope_label="heavy" calc_neutral_mass="985.522049" precursor_mz="493.7683" collision_energy="18.54951" modified_sequence="SSAAPPPPPR[+10.0]">
        <precursor_results>
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="18.6450176" start_time="18.3777161" end_time="19.0014172" fwhm="0.315500379" area="4.359072E+10" background="2.27518157E+09" height="2.180068E+09" mass_error_ppm="0.6428878" truncated="0" identified="false" isotope_dotp="0.9738929" user_set="FALSE" />
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="18.4602032" start_time="18.3297043" end_time="18.8082047" fwhm="0.271300554" area="4.49507451E+10" background="1.6949504E+09" height="2.818151E+09" mass_error_ppm="0.6988945" truncated="0" identified="false" isotope_dotp="0.975168169" user_set="FALSE" />
          <precursor_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="19.7548676" start_time="19.5964661" end_time="20.239666" fwhm="0.20844698" area="9.830638E+10" background="6.071546E+09" height="4.796676E+09" mass_error_ppm="-0.0357872546" truncated="0" identified="false" isotope_dotp="0.993022859" user_set="FALSE" />
          <precursor_peak replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="20.726902" start_time="20.4369011" end_time="21.1155014" fwhm="0.15387471" area="1.89083341E+10" background="357585024" height="1.20254144E+09" mass_error_ppm="0.215293348" truncated="0" identified="false" isotope_dotp="0.995888352" user_set="FALSE" />
          <precursor_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="19.8964272" start_time="19.6354256" end_time="20.247427" fwhm="0.163680226" area="6.26104074E+10" background="6.963917E+08" height="3.66416179E+09" mass_error_ppm="0.09931502" truncated="0" identified="false" isotope_dotp="0.99424845" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="20.9372749" start_time="20.598875" end_time="22.0028763" fwhm="0.559803963" area="2.0305381E+11" background="6.27869235E+09" height="3.78633062E+09" mass_error_ppm="0.9581369" truncated="0" identified="false" isotope_dotp="0.988735557" user_set="TRUE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="21.1151047" start_time="20.8847046" end_time="21.8243046" fwhm="0.458143353" area="1.708571E+11" background="6.34497126E+09" height="4.21624934E+09" mass_error_ppm="0.5656117" truncated="0" identified="false" isotope_dotp="0.983668566" user_set="FALSE" />
        </precursor_results>
        <transition fragment_type="precursor" isotope_dist_rank="1" isotope_dist_proportion="0.6034911">
          <precursor_mz>493.7683</precursor_mz>
          <product_mz>493.7683</product_mz>
          <collision_energy>18.54951</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="1.2" retention_time="18.6450176" start_time="18.3777161" end_time="19.0014172" area="2.700986E+10" background="1.40691226E+09" height="2.180068E+09" fwhm="0.1792779" truncated="false" identified="false" rank="1" points_across="88" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="1.3" retention_time="18.4602032" start_time="18.3297043" end_time="18.8082047" area="2.78471619E+10" background="1.03306445E+09" height="2.818151E+09" fwhm="0.141947851" truncated="false" identified="false" rank="1" points_across="54" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="0.2" retention_time="19.7548676" start_time="19.5964661" end_time="20.239666" area="6.24076554E+10" background="3.59054259E+09" height="4.796676E+09" fwhm="0.199088" truncated="false" identified="false" rank="1" points_across="129" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="0.6" retention_time="20.726902" start_time="20.4369011" end_time="21.1155014" area="1.18891028E+10" background="224819648" height="1.20254144E+09" fwhm="0.141316235" truncated="false" identified="false" rank="1" points_across="119" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="0.4" retention_time="19.8964272" start_time="19.6354256" end_time="20.247427" area="3.95729879E+10" background="485884736" height="3.66416179E+09" fwhm="0.16028735" truncated="false" identified="false" rank="1" points_across="61" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="1.2" retention_time="20.9372749" start_time="20.598875" end_time="22.0028763" area="1.28664314E+11" background="4.318322E+09" height="3.78633062E+09" fwhm="0.559803963" truncated="false" identified="false" rank="1" points_across="211" user_set="TRUE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.7" retention_time="21.1151047" start_time="20.8847046" end_time="21.8243046" area="1.09864149E+11" background="3.34329344E+09" height="4.21624934E+09" fwhm="0.458143353" truncated="false" identified="false" rank="1" points_across="110" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="1" isotope_dist_rank="2" isotope_dist_proportion="0.2713201">
          <precursor_mz>493.7683</precursor_mz>
          <product_mz>494.269797</product_mz>
          <collision_energy>18.54951</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-0.6" retention_time="18.6450176" start_time="18.3777161" end_time="19.0014172" area="1.15257477E+10" background="603068160" height="922015744" fwhm="0.1808065" truncated="false" identified="false" rank="2" points_across="88" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-0.6" retention_time="18.4747047" start_time="18.3297043" end_time="18.8082047" area="1.19238093E+10" background="442218560" height="1.19851046E+09" fwhm="0.143897712" truncated="false" identified="false" rank="2" points_across="54" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-0.5" retention_time="19.7356663" start_time="19.5964661" end_time="20.239666" area="2.693772E+10" background="2.016863E+09" height="2.07411162E+09" fwhm="0.199777484" truncated="false" identified="false" rank="2" points_across="129" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.5" retention_time="20.726902" start_time="20.4369011" end_time="21.1155014" area="5.252557E+09" background="9.779773E+07" height="515196768" fwhm="0.143282816" truncated="false" identified="false" rank="2" points_across="119" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.5" retention_time="19.8874264" start_time="19.6354256" end_time="20.247427" area="1.71988E+10" background="1.801117E+08" height="1.610685E+09" fwhm="0.1517475" truncated="false" identified="false" rank="2" points_across="61" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.5" retention_time="20.9372749" start_time="20.598875" end_time="22.0028763" area="5.69307668E+10" background="1.54154688E+09" height="1.6846473E+09" fwhm="0.557901859" truncated="false" identified="false" rank="2" points_across="211" user_set="TRUE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.3" retention_time="21.1331043" start_time="20.8847046" end_time="21.8243046" area="4.663304E+10" background="2.08811955E+09" height="1.8599744E+09" fwhm="0.4394999" truncated="false" identified="false" rank="2" points_across="110" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="2" isotope_dist_rank="3" isotope_dist_proportion="0.07579697">
          <precursor_mz>493.7683</precursor_mz>
          <product_mz>494.771095</product_mz>
          <collision_energy>18.54951</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="0.5" retention_time="18.6351166" start_time="18.3777161" end_time="19.0014172" area="3.95306061E+09" background="1.902779E+08" height="239883632" fwhm="0.315500379" truncated="false" identified="false" rank="3" points_across="88" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="0.5" retention_time="18.4747047" start_time="18.3297043" end_time="18.8082047" area="4.07436186E+09" background="163906816" height="311416672" fwhm="0.271300554" truncated="false" identified="false" rank="3" points_across="54" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-0.3" retention_time="19.7500668" start_time="19.5964661" end_time="20.239666" area="7.385854E+09" background="364805216" height="628607552" fwhm="0.185442492" truncated="false" identified="false" rank="3" points_across="129" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.1" retention_time="20.726902" start_time="20.4369011" end_time="21.1155014" area="1.44207782E+09" background="23637136" height="139884688" fwhm="0.15387471" truncated="false" identified="false" rank="3" points_across="119" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.1" retention_time="19.8784256" start_time="19.6354256" end_time="20.247427" area="4.76916736E+09" background="3.039526E+07" height="4.272779E+08" fwhm="0.163680226" truncated="false" identified="false" rank="3" points_across="61" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.7" retention_time="20.9372749" start_time="20.598875" end_time="22.0028763" area="1.480716E+10" background="418823168" height="4.457723E+08" fwhm="0.550430238" truncated="false" identified="false" rank="3" points_across="211" user_set="TRUE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.4" retention_time="21.1331043" start_time="20.8847046" end_time="21.8243046" area="1.2314495E+10" background="750606464" height="488330464" fwhm="0.425925732" truncated="false" identified="false" rank="3" points_across="110" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="3" isotope_dist_rank="5" isotope_dist_proportion="0.0157492422">
          <precursor_mz>493.7683</precursor_mz>
          <product_mz>495.272363</product_mz>
          <collision_energy>18.54951</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="0.5" retention_time="18.8133163" start_time="18.3777161" end_time="19.0014172" area="1.10205222E+09" background="74923384" height="7.166341E+07" fwhm="0.2983881" truncated="false" identified="false" rank="4" points_across="88" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="0.3" retention_time="18.6197033" start_time="18.3297043" end_time="18.8082047" area="1.105414E+09" background="55760696" height="85357624" fwhm="0.263411522" truncated="false" identified="false" rank="4" points_across="54" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-0.2" retention_time="19.7308674" start_time="19.5964661" end_time="20.239666" area="1.575151E+09" background="9.933507E+07" height="120007920" fwhm="0.20844698" truncated="false" identified="false" rank="4" points_across="129" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.9" retention_time="20.7095013" start_time="20.4369011" end_time="21.1155014" area="3.245965E+08" background="11330482" height="3.407645E+07" fwhm="0.110520832" truncated="false" identified="false" rank="4" points_across="119" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.5" retention_time="19.8784256" start_time="19.6354256" end_time="20.247427" area="1.06944954E+09" background="0" height="9.648808E+07" fwhm="0.155534267" truncated="false" identified="false" rank="4" points_across="61" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.5" retention_time="20.9228764" start_time="20.598875" end_time="22.0028763" area="2.651563E+09" background="0" height="8.23121E+07" fwhm="0.5508914" truncated="false" identified="false" rank="4" points_across="211" user_set="TRUE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.4" retention_time="21.2015038" start_time="20.8847046" end_time="21.8243046" area="2.04542451E+09" background="162951936" height="8.146794E+07" fwhm="0.406694859" truncated="false" identified="false" rank="4" points_across="110" user_set="FALSE" />
          </transition_results>
        </transition>
      </precursor>
    </peptide>
    <peptide auto_manage_children="false" standard_type="iRT" sequence="HVLTSIGEK" modified_sequence="HVLTSIGEK" calc_neutral_pep_mass="982.544745" num_missed_cleavages="0" rt_calculator_score="5.74466530910496" predicted_retention_time="22.22" avg_measured_retention_time="22.3298569">
      <implicit_modifications>
        <implicit_heavy_modifications>
          <implicit_modification index_aa="8" modification_name="Label:13C(6)15N(2) (C-term K)" mass_diff="+8" />
        </implicit_heavy_modifications>
      </implicit_modifications>
      <peptide_results>
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="19.694416" predicted_retention_time="20.49" />
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="19.5912037" predicted_retention_time="20.36" />
        <peptide_result replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="21.8476677" predicted_retention_time="21.62" />
        <peptide_result replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="23.1919022" predicted_retention_time="22.79" />
        <peptide_result replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="21.1474266" predicted_retention_time="21.62" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="25.1960754" predicted_retention_time="24.2" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="25.6403046" predicted_retention_time="24.42" />
      </peptide_results>
      <precursor charge="2" isotope_label="heavy" calc_neutral_mass="990.558944" precursor_mz="496.286748" collision_energy="18.676067" modified_sequence="HVLTSIGEK[+8.0]">
        <precursor_results>
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="19.694416" start_time="19.5360165" end_time="19.8726158" fwhm="0.109634273" area="4.99391365E+10" background="8.766399E+08" height="4.94938368E+09" mass_error_ppm="0.345182866" truncated="0" identified="false" isotope_dotp="0.98811084" user_set="FALSE" />
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="19.5912037" start_time="19.4462032" end_time="19.7507038" fwhm="0.0997315" area="4.8850944E+10" background="687934336" height="5.036214E+09" mass_error_ppm="0.383925945" truncated="0" identified="false" isotope_dotp="0.9886437" user_set="FALSE" />
          <precursor_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="21.8476677" start_time="21.7084675" end_time="22.130867" fwhm="0.113555357" area="1.238E+11" background="2.90527181E+09" height="1.13853061E+10" mass_error_ppm="0.642231047" truncated="0" identified="false" isotope_dotp="0.9906677" user_set="FALSE" />
          <precursor_peak replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="23.1919022" start_time="23.0701" end_time="23.4529018" fwhm="0.113046758" area="2.23627284E+10" background="292161152" height="1.9608457E+09" mass_error_ppm="-0.733067334" truncated="0" identified="false" isotope_dotp="0.985456467" user_set="FALSE" />
          <precursor_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="21.1474266" start_time="20.9764271" end_time="21.4174271" fwhm="0.149452984" area="4.62673756E+10" background="768606656" height="2.86277274E+09" mass_error_ppm="0.308465421" truncated="0" identified="false" isotope_dotp="0.9947227" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="25.1960754" start_time="24.926075" end_time="25.5920753" fwhm="0.311027825" area="1.83109583E+11" background="8.041423E+08" height="6.89965466E+09" mass_error_ppm="0.6806685" truncated="0" identified="false" isotope_dotp="0.9778921" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="25.6403046" start_time="25.4171047" end_time="25.9283047" fwhm="0.251541883" area="1.60329236E+11" background="1.70267686E+09" height="7.47400243E+09" mass_error_ppm="0.232597649" truncated="0" identified="false" isotope_dotp="0.9763431" user_set="FALSE" />
        </precursor_results>
        <transition fragment_type="precursor" isotope_dist_rank="1" isotope_dist_proportion="0.602819741">
          <precursor_mz>496.286748</precursor_mz>
          <product_mz>496.286748</product_mz>
          <collision_energy>18.676067</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="1.4" retention_time="19.694416" start_time="19.5360165" end_time="19.8726158" area="3.06991862E+10" background="5.472191E+08" height="4.94938368E+09" fwhm="0.0991533846" truncated="false" identified="false" rank="1" points_across="42" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="1.4" retention_time="19.5912037" start_time="19.4462032" end_time="19.7507038" area="3.00371579E+10" background="374176480" height="5.036214E+09" fwhm="0.09205719" truncated="false" identified="false" rank="1" points_across="26" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="1.1" retention_time="21.8476677" start_time="21.7084675" end_time="22.130867" area="7.69993441E+10" background="1.41310746E+09" height="1.13853061E+10" fwhm="0.0948770642" truncated="false" identified="false" rank="1" points_across="79" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.6" retention_time="23.1919022" start_time="23.0701" end_time="23.4529018" area="1.41649172E+10" background="191082352" height="1.9608457E+09" fwhm="0.111285165" truncated="false" identified="false" rank="1" points_across="60" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="0.6" retention_time="21.1474266" start_time="20.9764271" end_time="21.4174271" area="2.90466017E+10" background="364562336" height="2.86277274E+09" fwhm="0.149452984" truncated="false" identified="false" rank="1" points_across="60" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="1.2" retention_time="25.1960754" start_time="24.926075" end_time="25.5920753" area="1.19463641E+11" background="4.957075E+08" height="6.89965466E+09" fwhm="0.311027825" truncated="false" identified="false" rank="1" points_across="71" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.7" retention_time="25.6403046" start_time="25.4171047" end_time="25.9283047" area="1.0491981E+11" background="1.26094554E+09" height="7.47400243E+09" fwhm="0.245424345" truncated="false" identified="false" rank="1" points_across="47" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="1" isotope_dist_rank="2" isotope_dist_proportion="0.274366617">
          <precursor_mz>496.286748</precursor_mz>
          <product_mz>496.788216</product_mz>
          <collision_energy>18.676067</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-1.3" retention_time="19.694416" start_time="19.5360165" end_time="19.8726158" area="1.47384064E+10" background="237172672" height="2.20801741E+09" fwhm="0.107949451" truncated="false" identified="false" rank="2" points_across="42" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-1.2" retention_time="19.5912037" start_time="19.4462032" end_time="19.7507038" area="1.44121682E+10" background="219717536" height="2.32352358E+09" fwhm="0.09589368" truncated="false" identified="false" rank="2" points_across="26" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="0" retention_time="21.8476677" start_time="21.7084675" end_time="22.130867" area="3.56767949E+10" background="7.916188E+08" height="5.02067251E+09" fwhm="0.09729701" truncated="false" identified="false" rank="2" points_across="79" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-1" retention_time="23.1977" start_time="23.0701" end_time="23.4529018" area="6.29691E+09" background="7.749295E+07" height="860189056" fwhm="0.111554928" truncated="false" identified="false" rank="2" points_across="60" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="0.1" retention_time="21.1474266" start_time="20.9764271" end_time="21.4174271" area="1.27860879E+10" background="186503936" height="1.21947827E+09" fwhm="0.1487104" truncated="false" identified="false" rank="2" points_across="60" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="-0.6" retention_time="25.1960754" start_time="24.926075" end_time="25.5920753" area="4.789778E+10" background="229356656" height="2.763149E+09" fwhm="0.309217632" truncated="false" identified="false" rank="2" points_across="71" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="-1" retention_time="25.6187038" start_time="25.4171047" end_time="25.9283047" area="4.18480456E+10" background="316828160" height="2.93588582E+09" fwhm="0.244961262" truncated="false" identified="false" rank="2" points_across="47" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="2" isotope_dist_rank="3" isotope_dist_proportion="0.07842033">
          <precursor_mz>496.286748</precursor_mz>
          <product_mz>497.289497</product_mz>
          <collision_energy>18.676067</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-1.5" retention_time="19.694416" start_time="19.5360165" end_time="19.8726158" area="3.64395981E+09" background="92248104" height="5.473109E+08" fwhm="0.108051278" truncated="false" identified="false" rank="3" points_across="42" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-1.4" retention_time="19.6057034" start_time="19.4462032" end_time="19.7507038" area="3.6016256E+09" background="8.361442E+07" height="5.718846E+08" fwhm="0.0997315" truncated="false" identified="false" rank="3" points_across="26" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-0.5" retention_time="21.8380661" start_time="21.7084675" end_time="22.130867" area="9.269585E+09" background="520373824" height="1.37920013E+09" fwhm="0.0984732956" truncated="false" identified="false" rank="3" points_across="79" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.7" retention_time="23.1977" start_time="23.0701" end_time="23.4529018" area="1.56728538E+09" background="23585868" height="215447920" fwhm="0.113046758" truncated="false" identified="false" rank="3" points_across="60" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-1" retention_time="21.1384258" start_time="20.9764271" end_time="21.4174271" area="3.63515162E+09" background="170555648" height="378162752" fwhm="0.143783659" truncated="false" identified="false" rank="3" points_across="60" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.8" retention_time="25.2356758" start_time="24.926075" end_time="25.5920753" area="1.316887E+10" background="7.907813E+07" height="771628864" fwhm="0.295168519" truncated="false" identified="false" rank="3" points_across="71" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.6" retention_time="25.6403046" start_time="25.4171047" end_time="25.9283047" area="1.13427825E+10" background="124903208" height="775551552" fwhm="0.251541883" truncated="false" identified="false" rank="3" points_across="47" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="3" isotope_dist_rank="5" isotope_dist_proportion="0.0166879967">
          <precursor_mz>496.286748</precursor_mz>
          <product_mz>497.790752</product_mz>
          <collision_energy>18.676067</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-1.3" retention_time="19.694416" start_time="19.5360165" end_time="19.8726158" area="857583040" background="0" height="126741448" fwhm="0.109634273" truncated="false" identified="false" rank="4" points_across="42" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-1.2" retention_time="19.5912037" start_time="19.4462032" end_time="19.7507038" area="7.999956E+08" background="10425908" height="127392952" fwhm="0.09752139" truncated="false" identified="false" rank="4" points_across="26" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-0.3" retention_time="21.8284664" start_time="21.7084675" end_time="22.130867" area="1.85427866E+09" background="180171840" height="255306512" fwhm="0.113555357" truncated="false" identified="false" rank="4" points_across="79" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-1.5" retention_time="23.209301" start_time="23.0701" end_time="23.4529018" area="333617472" background="0" height="44842708" fwhm="0.112214111" truncated="false" identified="false" rank="4" points_across="60" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-1" retention_time="21.1384258" start_time="20.9764271" end_time="21.4174271" area="7.995332E+08" background="46984796" height="83228216" fwhm="0.143219575" truncated="false" identified="false" rank="4" points_across="60" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="-0.2" retention_time="25.2248764" start_time="24.926075" end_time="25.5920753" area="2.57930829E+09" background="0" height="152903680" fwhm="0.2876323" truncated="false" identified="false" rank="4" points_across="71" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="-0.5" retention_time="25.5863037" start_time="25.4171047" end_time="25.9283047" area="2.21858253E+09" background="0" height="163938928" fwhm="0.23663643" truncated="false" identified="false" rank="4" points_across="47" user_set="FALSE" />
          </transition_results>
        </transition>
      </precursor>
    </peptide>
    <peptide auto_manage_children="false" standard_type="iRT" sequence="GISNEGQNASIK" modified_sequence="GISNEGQNASIK" calc_neutral_pep_mass="1216.60478" num_missed_cleavages="0" rt_calculator_score="4.4877971122327835" predicted_retention_time="21.67" avg_measured_retention_time="22.1018143">
      <implicit_modifications>
        <implicit_heavy_modifications>
          <implicit_modification index_aa="11" modification_name="Label:13C(6)15N(2) (C-term K)" mass_diff="+8" />
        </implicit_heavy_modifications>
      </implicit_modifications>
      <peptide_results>
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="20.1696167" predicted_retention_time="19.98" />
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="19.9827042" predicted_retention_time="19.85" />
        <peptide_result replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="21.5548668" predicted_retention_time="21.08" />
        <peptide_result replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="22.2871017" predicted_retention_time="22.27" />
        <peptide_result replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="21.4804268" predicted_retention_time="21.09" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="24.4688759" predicted_retention_time="23.61" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="24.769104" predicted_retention_time="23.81" />
      </peptide_results>
      <precursor charge="2" isotope_label="heavy" calc_neutral_mass="1224.618979" precursor_mz="613.316765" collision_energy="22.889148" modified_sequence="GISNEGQNASIK[+8.0]">
        <precursor_results>
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="20.1696167" start_time="20.0508175" end_time="20.2884159" fwhm="0.07721377" area="3.922354E+10" background="503196960" height="4.660395E+09" mass_error_ppm="0.2914746" truncated="0" identified="false" isotope_dotp="0.9934436" user_set="FALSE" />
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="19.9827042" start_time="19.8522034" end_time="20.113203" fwhm="0.0923211947" area="4.251255E+10" background="2.714066E+08" height="4.255617E+09" mass_error_ppm="0.253462017" truncated="0" identified="false" isotope_dotp="0.9929711" user_set="FALSE" />
          <precursor_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="21.5548668" start_time="21.4588661" end_time="21.7660675" fwhm="0.0941347554" area="9.335159E+10" background="9.567233E+08" height="9.646157E+09" mass_error_ppm="-0.351094723" truncated="0" identified="false" isotope_dotp="0.983967245" user_set="FALSE" />
          <precursor_peak replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="22.2871017" start_time="22.1653" end_time="22.3915" fwhm="0.076650776" area="1.927781E+10" background="258876816" height="2.272448E+09" mass_error_ppm="-0.402301461" truncated="0" identified="false" isotope_dotp="0.9825899" user_set="FALSE" />
          <precursor_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="21.4804268" start_time="21.3364258" end_time="21.6334267" fwhm="0.08530193" area="6.90224E+10" background="1.03805248E+09" height="7.451994E+09" mass_error_ppm="0.5008385" truncated="0" identified="false" isotope_dotp="0.9859116" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="24.4688759" start_time="24.2672749" end_time="24.6848755" fwhm="0.149428755" area="1.27185314E+11" background="2.248666E+09" height="7.96659456E+09" mass_error_ppm="0.50788635" truncated="0" identified="false" isotope_dotp="0.989334345" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="24.769104" start_time="24.4451046" end_time="25.1363049" fwhm="0.235121548" area="1.465391E+11" background="1.656768E+09" height="6.16832563E+09" mass_error_ppm="0.5334319" truncated="0" identified="false" isotope_dotp="0.990418255" user_set="FALSE" />
        </precursor_results>
        <transition fragment_type="precursor" isotope_dist_rank="1" isotope_dist_proportion="0.549474955">
          <precursor_mz>613.316765</precursor_mz>
          <product_mz>613.316765</product_mz>
          <collision_energy>22.889148</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="0.8" retention_time="20.1696167" start_time="20.0508175" end_time="20.2884159" area="2.24235848E+10" background="2.663658E+08" height="4.660395E+09" fwhm="0.07721377" truncated="false" identified="false" rank="1" points_across="15" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="0.8" retention_time="19.9827042" start_time="19.8522034" end_time="20.113203" area="2.44441989E+10" background="124585184" height="4.255617E+09" fwhm="0.0898259655" truncated="false" identified="false" rank="1" points_across="17" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="0.4" retention_time="21.5548668" start_time="21.4588661" end_time="21.7660675" area="5.495226E+10" background="508790080" height="9.646157E+09" fwhm="0.0879251361" truncated="false" identified="false" rank="1" points_across="56" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="0.1" retention_time="22.2871017" start_time="22.1653" end_time="22.3915" area="1.1371135E+10" background="141480176" height="2.272448E+09" fwhm="0.0755766854" truncated="false" identified="false" rank="1" points_across="38" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="1.1" retention_time="21.4804268" start_time="21.3364258" end_time="21.6334267" area="4.03973775E+10" background="6.045198E+08" height="7.451994E+09" fwhm="0.08530193" truncated="false" identified="false" rank="1" points_across="31" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.7" retention_time="24.4688759" start_time="24.2672749" end_time="24.6848755" area="7.138363E+10" background="1.3080553E+09" height="7.96659456E+09" fwhm="0.149303913" truncated="false" identified="false" rank="1" points_across="33" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.6" retention_time="24.769104" start_time="24.4451046" end_time="25.1363049" area="8.24108646E+10" background="9.995327E+08" height="6.16832563E+09" fwhm="0.235121548" truncated="false" identified="false" rank="1" points_across="66" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="1" isotope_dist_rank="2" isotope_dist_proportion="0.295686245">
          <precursor_mz>613.316765</precursor_mz>
          <product_mz>613.818187</product_mz>
          <collision_energy>22.889148</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-0.6" retention_time="20.1696167" start_time="20.0508175" end_time="20.2884159" area="1.18691389E+10" background="154663616" height="2.475745E+09" fwhm="0.07669295" truncated="false" identified="false" rank="2" points_across="15" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-0.6" retention_time="19.9682045" start_time="19.8522034" end_time="20.113203" area="1.2770005E+10" background="9.385558E+07" height="2.21324134E+09" fwhm="0.0907565355" truncated="false" identified="false" rank="2" points_across="17" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-1.5" retention_time="21.5548668" start_time="21.4588661" end_time="21.7660675" area="2.73497682E+10" background="2.96307E+08" height="4.51154739E+09" fwhm="0.0941347554" truncated="false" identified="false" rank="2" points_across="56" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-1.1" retention_time="22.2871017" start_time="22.1653" end_time="22.3915" area="5.655488E+09" background="8.318654E+07" height="1.15946778E+09" fwhm="0.0718106255" truncated="false" identified="false" rank="2" points_across="38" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.4" retention_time="21.471426" start_time="21.3364258" end_time="21.6334267" area="2.02547753E+10" background="311991968" height="3.77379814E+09" fwhm="0.0829847157" truncated="false" identified="false" rank="2" points_across="31" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.6" retention_time="24.4652748" start_time="24.2672749" end_time="24.6848755" area="3.820341E+10" background="6.647762E+08" height="4.332949E+09" fwhm="0.147901639" truncated="false" identified="false" rank="2" points_across="33" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.8" retention_time="24.769104" start_time="24.4451046" end_time="25.1363049" area="4.393486E+10" background="517255840" height="3.328223E+09" fwhm="0.23315765" truncated="false" identified="false" rank="2" points_across="66" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="2" isotope_dist_rank="3" isotope_dist_proportion="0.100698136">
          <precursor_mz>613.316765</precursor_mz>
          <product_mz>614.31944</product_mz>
          <collision_energy>22.889148</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="0.3" retention_time="20.1696167" start_time="20.0508175" end_time="20.2884159" area="3.85086131E+09" background="65766108" height="795027456" fwhm="0.07648269" truncated="false" identified="false" rank="3" points_across="15" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="0" retention_time="19.9682045" start_time="19.8522034" end_time="20.113203" area="4.180307E+09" background="3.446713E+07" height="7.106043E+08" fwhm="0.0923211947" truncated="false" identified="false" rank="3" points_across="17" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-1.2" retention_time="21.5548668" start_time="21.4588661" end_time="21.7660675" area="8.689424E+09" background="151626224" height="1.41604749E+09" fwhm="0.09341863" truncated="false" identified="false" rank="3" points_across="56" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-1.1" retention_time="22.2871017" start_time="22.1653" end_time="22.3915" area="1.76302707E+09" background="22664994" height="359324032" fwhm="0.076650776" truncated="false" identified="false" rank="3" points_across="38" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.1" retention_time="21.471426" start_time="21.3364258" end_time="21.6334267" area="6.51204659E+09" background="104991480" height="1.23351142E+09" fwhm="0.0797835737" truncated="false" identified="false" rank="3" points_across="31" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="-0.4" retention_time="24.4688759" start_time="24.2672749" end_time="24.6848755" area="1.44592876E+10" background="262799120" height="1.60360819E+09" fwhm="0.149428755" truncated="false" identified="false" rank="3" points_across="33" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="-0.3" retention_time="24.769104" start_time="24.4451046" end_time="25.1363049" area="1.65157939E+10" background="139979520" height="1.27056614E+09" fwhm="0.230580419" truncated="false" identified="false" rank="3" points_across="66" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="3" isotope_dist_rank="4" isotope_dist_proportion="0.025602147">
          <precursor_mz>613.316765</precursor_mz>
          <product_mz>614.820676</product_mz>
          <collision_energy>22.889148</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-0.5" retention_time="20.1696167" start_time="20.0508175" end_time="20.2884159" area="1.07995533E+09" background="16401435" height="223396464" fwhm="0.07695518" truncated="false" identified="false" rank="4" points_across="15" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-1" retention_time="19.9682045" start_time="19.8522034" end_time="20.113203" area="1.11803955E+09" background="18498686" height="195520832" fwhm="0.09134151" truncated="false" identified="false" rank="4" points_across="17" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-1.4" retention_time="21.5548668" start_time="21.4588661" end_time="21.7660675" area="2.36013568E+09" background="0" height="393352640" fwhm="0.08809157" truncated="false" identified="false" rank="4" points_across="56" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-1.5" retention_time="22.2755013" start_time="22.1653" end_time="22.3915" area="4.881587E+08" background="11545107" height="9.584269E+07" fwhm="0.0764673948" truncated="false" identified="false" rank="4" points_across="38" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.6" retention_time="21.4624271" start_time="21.3364258" end_time="21.6334267" area="1.85820134E+09" background="16549257" height="347765248" fwhm="0.0807136446" truncated="false" identified="false" rank="4" points_across="31" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="-0.8" retention_time="24.4652748" start_time="24.2672749" end_time="24.6848755" area="3.138985E+09" background="13035484" height="358145920" fwhm="0.148774654" truncated="false" identified="false" rank="4" points_across="33" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="-0.4" retention_time="24.769104" start_time="24.4451046" end_time="25.1363049" area="3.677593E+09" background="0" height="268790752" fwhm="0.231909" truncated="false" identified="false" rank="4" points_across="66" user_set="FALSE" />
          </transition_results>
        </transition>
      </precursor>
    </peptide>
    <peptide auto_manage_children="false" standard_type="iRT" sequence="DIPVPKPK" modified_sequence="DIPVPKPK" calc_neutral_pep_mass="892.538203" num_missed_cleavages="0" rt_calculator_score="20.593259805616768" predicted_retention_time="28.65" avg_measured_retention_time="28.19697">
      <implicit_modifications>
        <implicit_heavy_modifications>
          <implicit_modification index_aa="7" modification_name="Label:13C(6)15N(2) (C-term K)" mass_diff="+8" />
        </implicit_heavy_modifications>
      </implicit_modifications>
      <peptide_results>
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="26.584816" predicted_retention_time="26.54" />
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="26.4642048" predicted_retention_time="26.42" />
        <peptide_result replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="28.1692677" predicted_retention_time="28.04" />
        <peptide_result replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="28.7309017" predicted_retention_time="28.86" />
        <peptide_result replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="27.8884258" predicted_retention_time="27.91" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="29.854475" predicted_retention_time="31.23" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="29.6867046" predicted_retention_time="31.56" />
      </peptide_results>
      <precursor charge="2" isotope_label="heavy" calc_neutral_mass="900.552402" precursor_mz="451.283477" collision_energy="17.05595" modified_sequence="DIPVPKPK[+8.0]">
        <precursor_results>
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="26.584816" start_time="26.2482166" end_time="26.8521175" fwhm="0.215595528" area="1.76529347E+10" background="207938752" height="914773568" mass_error_ppm="-0.03100034" truncated="0" identified="false" isotope_dotp="0.9821895" user_set="FALSE" />
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="26.4642048" start_time="26.1597042" end_time="26.7397041" fwhm="0.2124222" area="1.82098719E+10" background="5.915462E+07" height="949074560" mass_error_ppm="-0.09639624" truncated="0" identified="false" isotope_dotp="0.983472943" user_set="FALSE" />
          <precursor_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="28.1692677" start_time="27.8332672" end_time="28.7308674" fwhm="0.223345175" area="4.566284E+10" background="4.639664E+08" height="1.97301734E+09" mass_error_ppm="-0.528251767" truncated="0" identified="false" isotope_dotp="0.9957845" user_set="FALSE" />
          <precursor_peak replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="28.7309017" start_time="28.4177017" end_time="28.9977016" fwhm="0.188379169" area="6.690395E+09" background="107855288" height="379238368" mass_error_ppm="-0.266030222" truncated="0" identified="false" isotope_dotp="0.994620442" user_set="FALSE" />
          <precursor_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="27.8884258" start_time="27.5284271" end_time="28.2754269" fwhm="0.231029972" area="3.38619535E+10" background="6.419271E+08" height="1.56038E+09" mass_error_ppm="0.0752829" truncated="0" identified="false" isotope_dotp="0.982822537" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="29.854475" start_time="29.0408764" end_time="30.3044758" fwhm="0.489497244" area="6.52809421E+10" background="1.00693709E+09" height="1.41851315E+09" mass_error_ppm="-0.254208028" truncated="0" identified="false" isotope_dotp="0.990860939" user_set="TRUE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="29.6867046" start_time="28.898304" end_time="30.608305" fwhm="0.530864835" area="8.016514E+10" background="350352256" height="1.39739046E+09" mass_error_ppm="-0.235593423" truncated="0" identified="false" isotope_dotp="0.9839099" user_set="TRUE" />
        </precursor_results>
        <transition fragment_type="precursor" isotope_dist_rank="1" isotope_dist_proportion="0.6180615">
          <precursor_mz>451.283477</precursor_mz>
          <product_mz>451.283477</product_mz>
          <collision_energy>17.05595</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="0.3" retention_time="26.584816" start_time="26.2482166" end_time="26.8521175" area="1.16564685E+10" background="1.27139E+08" height="914773568" fwhm="0.204965681" truncated="false" identified="false" rank="1" points_across="52" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="0.2" retention_time="26.4642048" start_time="26.1597042" end_time="26.7397041" area="1.20133079E+10" background="47393716" height="949074560" fwhm="0.19990404" truncated="false" identified="false" rank="1" points_across="36" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-0.7" retention_time="28.1692677" start_time="27.8332672" end_time="28.7308674" area="2.90857882E+10" background="412473152" height="1.97301734E+09" fwhm="0.223345175" truncated="false" identified="false" rank="1" points_across="165" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.5" retention_time="28.7309017" start_time="28.4177017" end_time="28.9977016" area="4.2902423E+09" background="75445096" height="379238368" fwhm="0.188379169" truncated="false" identified="false" rank="1" points_across="92" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="0.4" retention_time="27.8884258" start_time="27.5284271" end_time="28.2754269" area="2.23230976E+10" background="4.025586E+08" height="1.56038E+09" fwhm="0.218155608" truncated="false" identified="false" rank="1" points_across="65" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="-0.4" retention_time="29.854475" start_time="29.0408764" end_time="30.3044758" area="4.23625974E+10" background="517621984" height="1.41851315E+09" fwhm="0.489497244" truncated="false" identified="false" rank="1" points_across="119" user_set="TRUE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="-0.2" retention_time="29.6867046" start_time="28.898304" end_time="30.608305" area="5.281395E+10" background="249128224" height="1.39739046E+09" fwhm="0.489034832" truncated="false" identified="false" rank="1" points_across="182" user_set="TRUE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="1" isotope_dist_rank="2" isotope_dist_proportion="0.26916638">
          <precursor_mz>451.283477</precursor_mz>
          <product_mz>451.784987</product_mz>
          <collision_energy>17.05595</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-0.7" retention_time="26.584816" start_time="26.2482166" end_time="26.8521175" area="4.46308966E+09" background="8.079974E+07" height="344241056" fwhm="0.209125772" truncated="false" identified="false" rank="2" points_across="52" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-0.7" retention_time="26.4497032" start_time="26.1597042" end_time="26.7397041" area="4.65135053E+09" background="0" height="364020960" fwhm="0.2016749" truncated="false" identified="false" rank="2" points_across="36" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="0" retention_time="28.1692677" start_time="27.8332672" end_time="28.7308674" area="1.26975631E+10" background="41740188" height="9.408261E+08" fwhm="0.204351127" truncated="false" identified="false" rank="2" points_across="165" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="0.4" retention_time="28.736702" start_time="28.4177017" end_time="28.9977016" area="1.83608858E+09" background="30999374" height="165816544" fwhm="0.186014324" truncated="false" identified="false" rank="2" points_across="92" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.6" retention_time="27.8974266" start_time="27.5284271" end_time="28.2754269" area="8.587791E+09" background="193995872" height="598040064" fwhm="0.229934767" truncated="false" identified="false" rank="2" points_across="65" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0" retention_time="29.6060753" start_time="29.0408764" end_time="30.3044758" area="1.73333524E+10" background="4.428839E+08" height="6.108229E+08" fwhm="0.475052238" truncated="false" identified="false" rank="2" points_across="119" user_set="TRUE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="-0.4" retention_time="29.6759033" start_time="28.898304" end_time="30.608305" area="2.05471867E+10" background="101224032" height="5.404995E+08" fwhm="0.530864835" truncated="false" identified="false" rank="2" points_across="182" user_set="TRUE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="2" isotope_dist_rank="3" isotope_dist_proportion="0.07104732">
          <precursor_mz>451.283477</precursor_mz>
          <product_mz>452.286313</product_mz>
          <collision_energy>17.05595</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-0.6" retention_time="26.594717" start_time="26.2482166" end_time="26.8521175" area="1.287845E+09" background="0" height="9.7796E+07" fwhm="0.215595528" truncated="false" identified="false" rank="3" points_across="52" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-0.6" retention_time="26.4642048" start_time="26.1597042" end_time="26.7397041" area="1.29472333E+09" background="11760904" height="9.929408E+07" fwhm="0.2124222" truncated="false" identified="false" rank="3" points_across="36" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-1" retention_time="28.1452675" start_time="27.8332672" end_time="28.7308674" area="3.28916224E+09" background="9753043" height="2.339884E+08" fwhm="0.208143622" truncated="false" identified="false" rank="3" points_across="165" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.7" retention_time="28.736702" start_time="28.4177017" end_time="28.9977016" area="478452736" background="1410816.63" height="4.363492E+07" fwhm="0.175189838" truncated="false" identified="false" rank="3" points_across="92" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.4" retention_time="27.8704262" start_time="27.5284271" end_time="28.2754269" area="2.48194918E+09" background="4.537258E+07" height="1.733798E+08" fwhm="0.231029972" truncated="false" identified="false" rank="3" points_across="65" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0" retention_time="29.6456757" start_time="29.0408764" end_time="30.3044758" area="4.70974259E+09" background="4.643122E+07" height="173282112" fwhm="0.476187527" truncated="false" identified="false" rank="3" points_across="119" user_set="TRUE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0" retention_time="29.6867046" start_time="28.898304" end_time="30.608305" area="5.756859E+09" background="0" height="159864336" fwhm="0.453220159" truncated="false" identified="false" rank="3" points_across="182" user_set="TRUE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="3" isotope_dist_rank="5" isotope_dist_proportion="0.0139711695">
          <precursor_mz>451.283477</precursor_mz>
          <product_mz>452.787598</product_mz>
          <collision_energy>17.05595</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-0.6" retention_time="26.584816" start_time="26.2482166" end_time="26.8521175" area="245529872" background="0" height="19679028" fwhm="0.208122611" truncated="false" identified="false" rank="4" points_across="52" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-0.5" retention_time="26.4932041" start_time="26.1597042" end_time="26.7397041" area="250490448" background="0" height="19628146" fwhm="0.199119925" truncated="false" identified="false" rank="4" points_across="36" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-0.8" retention_time="28.1788673" start_time="27.8332672" end_time="28.7308674" area="5.903251E+08" background="0" height="46774776" fwhm="0.189885348" truncated="false" identified="false" rank="4" points_across="165" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.4" retention_time="28.7309017" start_time="28.4177017" end_time="28.9977016" area="8.561142E+07" background="0" height="8249142" fwhm="0.167983428" truncated="false" identified="false" rank="4" points_across="92" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.5" retention_time="27.879427" start_time="27.5284271" end_time="28.2754269" area="469116736" background="0" height="3.465588E+07" fwhm="0.212244615" truncated="false" identified="false" rank="4" points_across="65" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.4" retention_time="29.6240749" start_time="29.0408764" end_time="30.3044758" area="8.752486E+08" background="0" height="3.90179E+07" fwhm="0.2634079" truncated="false" identified="false" rank="4" points_across="119" user_set="TRUE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="-0.1" retention_time="29.6867046" start_time="28.898304" end_time="30.608305" area="1.04713888E+09" background="0" height="31346082" fwhm="0.30227834" truncated="false" identified="false" rank="4" points_across="182" user_set="TRUE" />
          </transition_results>
        </transition>
      </precursor>
    </peptide>
    <peptide auto_manage_children="false" standard_type="iRT" sequence="IGDYAGIK" modified_sequence="IGDYAGIK" calc_neutral_pep_mass="835.443969" num_missed_cleavages="0" rt_calculator_score="24.532460261470177" predicted_retention_time="30.36" avg_measured_retention_time="30.283514">
      <implicit_modifications>
        <implicit_heavy_modifications>
          <implicit_modification index_aa="7" modification_name="Label:13C(6)15N(2) (C-term K)" mass_diff="+8" />
        </implicit_heavy_modifications>
      </implicit_modifications>
      <peptide_results>
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="28.0599174" predicted_retention_time="28.14" />
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="27.899704" predicted_retention_time="28.03" />
        <peptide_result replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="29.441267" predicted_retention_time="29.75" />
        <peptide_result replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="30.1055012" predicted_retention_time="30.48" />
        <peptide_result replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="29.4094257" predicted_retention_time="29.57" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="33.3572769" predicted_retention_time="33.09" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="33.7115059" predicted_retention_time="33.46" />
      </peptide_results>
      <precursor charge="2" isotope_label="heavy" calc_neutral_mass="843.458168" precursor_mz="422.73636" collision_energy="16.028253" modified_sequence="IGDYAGIK[+8.0]">
        <precursor_results>
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="28.0599174" start_time="27.9213161" end_time="28.1787167" fwhm="0.0613642521" area="4.8522453E+10" background="1.23804557E+09" height="8.14971341E+09" mass_error_ppm="-0.0183239821" truncated="0" identified="false" isotope_dotp="0.982976556" user_set="FALSE" />
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="27.899704" start_time="27.7547035" end_time="28.0302048" fwhm="0.0897591561" area="4.621726E+10" background="899789248" height="5.563025E+09" mass_error_ppm="-0.0897854343" truncated="0" identified="false" isotope_dotp="0.983834147" user_set="FALSE" />
          <precursor_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="29.441267" start_time="29.3164673" end_time="29.7196674" fwhm="0.108925179" area="7.84591749E+10" background="2.49275776E+09" height="7.5293696E+09" mass_error_ppm="-0.0180831514" truncated="0" identified="false" isotope_dotp="0.982773542" user_set="FALSE" />
          <precursor_peak replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="30.1055012" start_time="29.9547" end_time="30.279501" fwhm="0.126656666" area="1.907037E+10" background="325942656" height="1.82444237E+09" mass_error_ppm="-0.826137543" truncated="0" identified="false" isotope_dotp="0.9847383" user_set="FALSE" />
          <precursor_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="29.4094257" start_time="29.247427" end_time="29.5534267" fwhm="0.1055423" area="4.735122E+10" background="8.31361E+08" height="5.08185754E+09" mass_error_ppm="0.232275739" truncated="0" identified="false" isotope_dotp="0.977382" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="33.3572769" start_time="33.1340752" end_time="33.8720741" fwhm="0.3098436" area="1.26365352E+11" background="1.48562368E+09" height="4.81128858E+09" mass_error_ppm="0.188585639" truncated="0" identified="false" isotope_dotp="0.977546751" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="33.7115059" start_time="33.5675049" end_time="34.0031052" fwhm="0.24316746" area="1.10394425E+11" background="1.10766682E+09" height="5.315124E+09" mass_error_ppm="-0.5987584" truncated="0" identified="false" isotope_dotp="0.979268" user_set="FALSE" />
        </precursor_results>
        <transition fragment_type="precursor" isotope_dist_rank="1" isotope_dist_proportion="0.6461924">
          <precursor_mz>422.73636</precursor_mz>
          <product_mz>422.73636</product_mz>
          <collision_energy>16.028253</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="0.5" retention_time="28.0599174" start_time="27.9213161" end_time="28.1787167" area="3.34859837E+10" background="782120256" height="8.14971341E+09" fwhm="0.0517000929" truncated="false" identified="false" rank="1" points_across="17" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="0.5" retention_time="27.899704" start_time="27.7547035" end_time="28.0302048" area="3.18248468E+10" background="517644544" height="5.563025E+09" fwhm="0.0882803351" truncated="false" identified="false" rank="1" points_across="18" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="0" retention_time="29.441267" start_time="29.3164673" end_time="29.7196674" area="5.41674045E+10" background="5.395687E+08" height="7.5293696E+09" fwhm="0.106128559" truncated="false" identified="false" rank="1" points_across="76" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.6" retention_time="30.1055012" start_time="29.9547" end_time="30.279501" area="1.30961439E+10" background="216246576" height="1.82444237E+09" fwhm="0.117871329" truncated="false" identified="false" rank="1" points_across="51" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="0.8" retention_time="29.4094257" start_time="29.247427" end_time="29.5534267" area="3.30577674E+10" background="3.906974E+08" height="5.08185754E+09" fwhm="0.102085337" truncated="false" identified="false" rank="1" points_across="25" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.6" retention_time="33.3572769" start_time="33.1340752" end_time="33.8720741" area="8.812997E+10" background="1.06067923E+09" height="4.81128858E+09" fwhm="0.3098436" truncated="false" identified="false" rank="1" points_across="88" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="-0.3" retention_time="33.7115059" start_time="33.5675049" end_time="34.0031052" area="7.66727946E+10" background="692720448" height="5.315124E+09" fwhm="0.24316746" truncated="false" identified="false" rank="1" points_across="33" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="1" isotope_dist_rank="2" isotope_dist_proportion="0.2505091">
          <precursor_mz>422.73636</precursor_mz>
          <product_mz>423.237871</product_mz>
          <collision_energy>16.028253</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-1.1" retention_time="28.0599174" start_time="27.9213161" end_time="28.1787167" area="1.15785083E+10" background="3.557752E+08" height="2.67388365E+09" fwhm="0.0609726571" truncated="false" identified="false" rank="2" points_across="17" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-1.3" retention_time="27.899704" start_time="27.7547035" end_time="28.0302048" area="1.10126E+10" background="293874432" height="2.0031095E+09" fwhm="0.08530677" truncated="false" identified="false" rank="2" points_across="18" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="0.1" retention_time="29.4364662" start_time="29.3164673" end_time="29.7196674" area="1.8667178E+10" background="1.53524774E+09" height="2.767536E+09" fwhm="0.102992177" truncated="false" identified="false" rank="2" points_across="76" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-1.4" retention_time="30.1055012" start_time="29.9547" end_time="30.279501" area="4.5424855E+09" background="9.136659E+07" height="6.199712E+08" fwhm="0.120479748" truncated="false" identified="false" rank="2" points_across="51" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-1.2" retention_time="29.4004269" start_time="29.247427" end_time="29.5534267" area="1.09853174E+10" background="118403184" height="1.64426611E+09" fwhm="0.1055423" truncated="false" identified="false" rank="2" points_across="25" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="-1" retention_time="33.3572769" start_time="33.1340752" end_time="33.8720741" area="2.96663818E+10" background="339322272" height="1.6182272E+09" fwhm="0.3080325" truncated="false" identified="false" rank="2" points_across="88" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="-1.5" retention_time="33.6971054" start_time="33.5675049" end_time="34.0031052" area="2.58835968E+10" background="336079680" height="1.8286592E+09" fwhm="0.240301371" truncated="false" identified="false" rank="2" points_across="33" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="2" isotope_dist_rank="3" isotope_dist_proportion="0.0630055144">
          <precursor_mz>422.73636</precursor_mz>
          <product_mz>423.73915</product_mz>
          <collision_energy>16.028253</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-1.4" retention_time="28.0599174" start_time="27.9213161" end_time="28.1787167" area="2.91182029E+09" background="91155544" height="694145664" fwhm="0.0542224944" truncated="false" identified="false" rank="3" points_across="17" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-1.7" retention_time="27.899704" start_time="27.7547035" end_time="28.0302048" area="2.86437248E+09" background="7.667088E+07" height="491006080" fwhm="0.0897591561" truncated="false" identified="false" rank="3" points_across="18" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-0.6" retention_time="29.4364662" start_time="29.3164673" end_time="29.7196674" area="4.732094E+09" background="383430880" height="6.801905E+08" fwhm="0.106115669" truncated="false" identified="false" rank="3" points_across="76" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-1.2" retention_time="30.1055012" start_time="29.9547" end_time="30.279501" area="1.20610726E+09" background="17084274" height="164768560" fwhm="0.126656666" truncated="false" identified="false" rank="3" points_across="51" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.7" retention_time="29.4004269" start_time="29.247427" end_time="29.5534267" area="2.80411213E+09" background="250470384" height="432628352" fwhm="0.103186026" truncated="false" identified="false" rank="3" points_across="25" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0" retention_time="33.3572769" start_time="33.1340752" end_time="33.8720741" area="7.330828E+09" background="4.952388E+07" height="4.421354E+08" fwhm="0.2838677" truncated="false" identified="false" rank="3" points_across="88" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="-0.6" retention_time="33.6971054" start_time="33.5675049" end_time="34.0031052" area="6.76186931E+09" background="55747064" height="486857184" fwhm="0.233698815" truncated="false" identified="false" rank="3" points_across="33" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="3" isotope_dist_rank="5" isotope_dist_proportion="0.0118336491">
          <precursor_mz>422.73636</precursor_mz>
          <product_mz>424.240413</product_mz>
          <collision_energy>16.028253</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-1.5" retention_time="28.0599174" start_time="27.9213161" end_time="28.1787167" area="5.46139E+08" background="8994576" height="123107448" fwhm="0.0613642521" truncated="false" identified="false" rank="4" points_across="17" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-1.7" retention_time="27.899704" start_time="27.7547035" end_time="28.0302048" area="515439872" background="11599407" height="8.90456E+07" fwhm="0.08915028" truncated="false" identified="false" rank="4" points_across="18" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-0.5" retention_time="29.441267" start_time="29.3164673" end_time="29.7196674" area="892500736" background="34510252" height="123534832" fwhm="0.108925179" truncated="false" identified="false" rank="4" points_across="76" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.4" retention_time="30.0997" start_time="29.9547" end_time="30.279501" area="225633120" background="1245194.25" height="3.279076E+07" fwhm="0.113018438" truncated="false" identified="false" rank="4" points_across="51" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.6" retention_time="29.4094257" start_time="29.247427" end_time="29.5534267" area="504022880" background="71790024" height="7.895762E+07" fwhm="0.102129273" truncated="false" identified="false" rank="4" points_across="25" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.5" retention_time="33.3500748" start_time="33.1340752" end_time="33.8720741" area="1.23817114E+09" background="3.609827E+07" height="7.922056E+07" fwhm="0.2570038" truncated="false" identified="false" rank="4" points_across="88" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="-0.2" retention_time="33.7547035" start_time="33.5675049" end_time="34.0031052" area="1.07616614E+09" background="23119564" height="7.710422E+07" fwhm="0.234024107" truncated="false" identified="false" rank="4" points_across="33" user_set="FALSE" />
          </transition_results>
        </transition>
      </precursor>
    </peptide>
    <peptide auto_manage_children="false" standard_type="iRT" sequence="TASEFDSAIAQDK" modified_sequence="TASEFDSAIAQDK" calc_neutral_pep_mass="1381.636139" num_missed_cleavages="0" rt_calculator_score="38.002336054523795" predicted_retention_time="36.2" avg_measured_retention_time="35.8751564">
      <implicit_modifications>
        <implicit_heavy_modifications>
          <implicit_modification index_aa="12" modification_name="Label:13C(6)15N(2) (C-term K)" mass_diff="+8" />
        </implicit_heavy_modifications>
      </implicit_modifications>
      <peptide_results>
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="33.7821159" predicted_retention_time="33.63" />
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="33.7432022" predicted_retention_time="33.53" />
        <peptide_result replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="34.6588669" predicted_retention_time="35.57" />
        <peptide_result replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="35.383503" predicted_retention_time="35.99" />
        <peptide_result replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="35.1064262" predicted_retention_time="35.28" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="38.9840736" predicted_retention_time="39.47" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="39.4679031" predicted_retention_time="39.94" />
      </peptide_results>
      <precursor charge="2" isotope_label="heavy" calc_neutral_mass="1389.650338" precursor_mz="695.832445" collision_energy="25.859712" modified_sequence="TASEFDSAIAQDK[+8.0]">
        <precursor_results>
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="33.7821159" start_time="33.6336174" end_time="34.0296173" fwhm="0.11822173" area="3.596587E+10" background="2.03334323E+09" height="2.367096E+09" mass_error_ppm="0.105472177" truncated="0" identified="false" isotope_dotp="0.9860571" user_set="FALSE" />
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="33.7432022" start_time="33.5692024" end_time="34.0042038" fwhm="0.1271411" area="3.859312E+10" background="1.94827891E+09" height="2.4677015E+09" mass_error_ppm="0.224423915" truncated="0" identified="false" isotope_dotp="0.9841806" user_set="FALSE" />
          <precursor_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="34.6588669" start_time="34.5340652" end_time="34.96607" fwhm="0.1128952" area="7.64264858E+10" background="4.919244E+09" height="5.487477E+09" mass_error_ppm="0.4558525" truncated="0" identified="false" isotope_dotp="0.982596338" user_set="FALSE" />
          <precursor_peak replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="35.383503" start_time="35.226902" end_time="35.6387024" fwhm="0.110215172" area="1.51977411E+10" background="7.260776E+08" height="1.07893325E+09" mass_error_ppm="-0.728266656" truncated="0" identified="false" isotope_dotp="0.987126946" user_set="FALSE" />
          <precursor_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="35.1064262" start_time="34.9354248" end_time="35.457428" fwhm="0.133079737" area="4.78917263E+10" background="3.037018E+09" height="2.816868E+09" mass_error_ppm="-0.162627712" truncated="0" identified="false" isotope_dotp="0.987717152" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="38.9840736" start_time="38.7248764" end_time="39.2324753" fwhm="0.216181591" area="9.945038E+10" background="1.15229632E+09" height="4.5192064E+09" mass_error_ppm="0.520260453" truncated="0" identified="false" isotope_dotp="0.9794918" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="39.4679031" start_time="39.1943054" end_time="39.7523041" fwhm="0.256276637" area="1.13801486E+11" background="1.11880883E+09" height="4.023835E+09" mass_error_ppm="-0.579634368" truncated="0" identified="false" isotope_dotp="0.9902781" user_set="FALSE" />
        </precursor_results>
        <transition fragment_type="precursor" isotope_dist_rank="1" isotope_dist_proportion="0.4965739">
          <precursor_mz>695.832445</precursor_mz>
          <product_mz>695.832445</product_mz>
          <collision_energy>25.859712</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="1.2" retention_time="33.7821159" start_time="33.6336174" end_time="34.0296173" area="1.81842964E+10" background="9.335983E+08" height="2.367096E+09" fwhm="0.114664227" truncated="false" identified="false" rank="1" points_across="19" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="1.4" retention_time="33.7432022" start_time="33.5692024" end_time="34.0042038" area="1.938323E+10" background="980948544" height="2.4677015E+09" fwhm="0.122121438" truncated="false" identified="false" rank="1" points_across="28" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="1.1" retention_time="34.6588669" start_time="34.5340652" end_time="34.96607" area="4.11195843E+10" background="2.6457152E+09" height="5.487477E+09" fwhm="0.110676765" truncated="false" identified="false" rank="1" points_across="74" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-1.2" retention_time="35.383503" start_time="35.226902" end_time="35.6387024" area="7.722003E+09" background="420334080" height="1.07893325E+09" fwhm="0.108496621" truncated="false" identified="false" rank="1" points_across="53" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.5" retention_time="35.1064262" start_time="34.9354248" end_time="35.457428" area="2.46846218E+10" background="1.33430784E+09" height="2.816868E+09" fwhm="0.13029936" truncated="false" identified="false" rank="1" points_across="30" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="1.1" retention_time="38.9840736" start_time="38.7248764" end_time="39.2324753" area="5.410971E+10" background="6.349294E+08" height="4.5192064E+09" fwhm="0.2019401" truncated="false" identified="false" rank="1" points_across="24" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="-0.9" retention_time="39.4679031" start_time="39.1943054" end_time="39.7523041" area="5.81862031E+10" background="609989056" height="4.023835E+09" fwhm="0.256276637" truncated="false" identified="false" rank="1" points_across="31" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="1" isotope_dist_rank="2" isotope_dist_proportion="0.31465584">
          <precursor_mz>695.832445</precursor_mz>
          <product_mz>696.33393</product_mz>
          <collision_energy>25.859712</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-0.9" retention_time="33.7821159" start_time="33.6336174" end_time="34.0296173" area="1.23205243E+10" background="8.174695E+08" height="1.65318426E+09" fwhm="0.113645554" truncated="false" identified="false" rank="2" points_across="19" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-0.8" retention_time="33.7432022" start_time="33.5692024" end_time="34.0042038" area="1.33553981E+10" background="725555264" height="1.70565811E+09" fwhm="0.122140907" truncated="false" identified="false" rank="2" points_across="28" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-0.4" retention_time="34.6732674" start_time="34.5340652" end_time="34.96607" area="2.378751E+10" background="1.43585574E+09" height="3.31844454E+09" fwhm="0.107213095" truncated="false" identified="false" rank="2" points_across="74" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="0" retention_time="35.383503" start_time="35.226902" end_time="35.6387024" area="4.83515238E+09" background="217590064" height="6.739095E+08" fwhm="0.108067691" truncated="false" identified="false" rank="2" points_across="53" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="0.4" retention_time="35.0974274" start_time="34.9354248" end_time="35.457428" area="1.50600591E+10" background="1.10289818E+09" height="1.72664154E+09" fwhm="0.128171772" truncated="false" identified="false" rank="2" points_across="30" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="-0.5" retention_time="38.9840736" start_time="38.7248764" end_time="39.2324753" area="3.00069028E+10" background="3.510232E+08" height="2.471484E+09" fwhm="0.203921407" truncated="false" identified="false" rank="2" points_across="24" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0" retention_time="39.4643059" start_time="39.1943054" end_time="39.7523041" area="3.61265766E+10" background="415671680" height="2.539246E+09" fwhm="0.242411032" truncated="false" identified="false" rank="2" points_across="31" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="2" isotope_dist_rank="3" isotope_dist_proportion="0.12276306">
          <precursor_mz>695.832445</precursor_mz>
          <product_mz>696.835238</product_mz>
          <collision_energy>25.859712</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-1.5" retention_time="33.7821159" start_time="33.6336174" end_time="34.0296173" area="4.20876058E+09" background="216124384" height="5.538843E+08" fwhm="0.115330048" truncated="false" identified="false" rank="3" points_across="19" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-1.4" retention_time="33.7287025" start_time="33.5692024" end_time="34.0042038" area="4.50347469E+09" background="159345232" height="5.664322E+08" fwhm="0.12440294" truncated="false" identified="false" rank="3" points_across="28" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="0.2" retention_time="34.6492653" start_time="34.5340652" end_time="34.96607" area="9.072311E+09" background="607933760" height="1.25449523E+09" fwhm="0.1128952" truncated="false" identified="false" rank="3" points_across="74" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.8" retention_time="35.3661" start_time="35.226902" end_time="35.6387024" area="2.122478E+09" background="7.045631E+07" height="2.911694E+08" fwhm="0.110131651" truncated="false" identified="false" rank="3" points_across="53" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.2" retention_time="35.0974274" start_time="34.9354248" end_time="35.457428" area="6.555306E+09" background="3.878422E+08" height="746790336" fwhm="0.129380643" truncated="false" identified="false" rank="3" points_across="30" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.8" retention_time="38.9840736" start_time="38.7248764" end_time="39.2324753" area="1.19710013E+10" background="143337824" height="9.606055E+08" fwhm="0.216181591" truncated="false" identified="false" rank="3" points_across="24" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="-0.8" retention_time="39.4643059" start_time="39.1943054" end_time="39.7523041" area="1.549813E+10" background="93148104" height="1.06052877E+09" fwhm="0.25092116" truncated="false" identified="false" rank="3" points_across="31" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="3" isotope_dist_rank="4" isotope_dist_proportion="0.03562245">
          <precursor_mz>695.832445</precursor_mz>
          <product_mz>697.336517</product_mz>
          <collision_energy>25.859712</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-0.5" retention_time="33.8118172" start_time="33.6336174" end_time="34.0296173" area="1.25228966E+09" background="6.615109E+07" height="160721728" fwhm="0.11822173" truncated="false" identified="false" rank="4" points_across="19" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-1.1" retention_time="33.7287025" start_time="33.5692024" end_time="34.0042038" area="1.35101709E+09" background="8.242988E+07" height="173636720" fwhm="0.1271411" truncated="false" identified="false" rank="4" points_across="28" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-1.1" retention_time="34.6732674" start_time="34.5340652" end_time="34.96607" area="2.447085E+09" background="229738848" height="351446368" fwhm="0.108098358" truncated="false" identified="false" rank="4" points_across="74" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.2" retention_time="35.3777" start_time="35.226902" end_time="35.6387024" area="5.181078E+08" background="17697056" height="7.069813E+07" fwhm="0.110215172" truncated="false" identified="false" rank="4" points_across="53" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.1" retention_time="35.0884247" start_time="34.9354248" end_time="35.457428" area="1.59173837E+09" background="211969808" height="178909952" fwhm="0.133079737" truncated="false" identified="false" rank="4" points_across="30" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="-0.7" retention_time="38.9840736" start_time="38.7248764" end_time="39.2324753" area="3.362763E+09" background="23005858" height="2.958278E+08" fwhm="0.179850683" truncated="false" identified="false" rank="4" points_across="24" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="-0.3" retention_time="39.4463043" start_time="39.1943054" end_time="39.7523041" area="3.990574E+09" background="0" height="265433584" fwhm="0.2548328" truncated="false" identified="false" rank="4" points_across="31" user_set="FALSE" />
          </transition_results>
        </transition>
      </precursor>
    </peptide>
    <peptide auto_manage_children="false" standard_type="iRT" sequence="SAAGAFGPELSR" modified_sequence="SAAGAFGPELSR" calc_neutral_pep_mass="1161.577836" num_missed_cleavages="0" rt_calculator_score="42.049898765308058" predicted_retention_time="37.95" avg_measured_retention_time="37.766655">
      <implicit_modifications>
        <implicit_heavy_modifications>
          <implicit_modification index_aa="11" modification_name="Label:13C(6)15N(4) (C-term R)" mass_diff="+10" />
        </implicit_heavy_modifications>
      </implicit_modifications>
      <peptide_results>
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="35.2374153" predicted_retention_time="35.28" />
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="35.106205" predicted_retention_time="35.18" />
        <peptide_result replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="36.74687" predicted_retention_time="37.32" />
        <peptide_result replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="37.3439026" predicted_retention_time="37.64" />
        <peptide_result replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="36.6634254" predicted_retention_time="36.99" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="41.3960762" predicted_retention_time="41.39" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="41.8727036" predicted_retention_time="41.88" />
      </peptide_results>
      <precursor charge="2" isotope_label="heavy" calc_neutral_mass="1171.586105" precursor_mz="586.800329" collision_energy="21.898663" modified_sequence="SAAGAFGPELSR[+10.0]">
        <precursor_results>
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="35.2374153" start_time="35.0493164" end_time="35.4354172" fwhm="0.12223842" area="4.98303754E+10" background="1.0875168E+09" height="3.52192384E+09" mass_error_ppm="-0.1994391" truncated="0" identified="false" isotope_dotp="0.9847176" user_set="FALSE" />
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="35.106205" start_time="34.9177055" end_time="35.2802048" fwhm="0.127749979" area="5.52734433E+10" background="9.536551E+08" height="4.06130842E+09" mass_error_ppm="0.0533410832" truncated="0" identified="false" isotope_dotp="0.9891648" user_set="FALSE" />
          <precursor_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="36.74687" start_time="36.5356674" end_time="37.14047" fwhm="0.15783143" area="1.123922E+11" background="2.79833626E+09" height="6.11399168E+09" mass_error_ppm="-0.435437471" truncated="0" identified="false" isotope_dotp="0.9928807" user_set="FALSE" />
          <precursor_peak replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="37.3439026" start_time="37.1699028" end_time="37.5295029" fwhm="0.127261952" area="2.24030781E+10" background="257511984" height="1.65638374E+09" mass_error_ppm="-0.9380284" truncated="0" identified="false" isotope_dotp="0.9849447" user_set="FALSE" />
          <precursor_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="36.6634254" start_time="36.4654274" end_time="36.879425" fwhm="0.142843887" area="6.351953E+10" background="1.3439511E+09" height="4.489522E+09" mass_error_ppm="0.2771058" truncated="0" identified="false" isotope_dotp="0.984241068" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="41.3960762" start_time="41.0000763" end_time="41.7488747" fwhm="0.2594778" area="1.4136669E+11" background="4.477494E+09" height="5.24950272E+09" mass_error_ppm="0.393835" truncated="0" identified="false" isotope_dotp="0.9773266" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="41.8727036" start_time="41.4083023" end_time="42.3803024" fwhm="0.264139146" area="1.40688884E+11" background="1.5497449E+09" height="4.389071E+09" mass_error_ppm="0.0108545665" truncated="0" identified="false" isotope_dotp="0.9723362" user_set="FALSE" />
        </precursor_results>
        <transition fragment_type="precursor" isotope_dist_rank="1" isotope_dist_proportion="0.5513175">
          <precursor_mz>586.800329</precursor_mz>
          <product_mz>586.800329</product_mz>
          <collision_energy>21.898663</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="0.4" retention_time="35.2374153" start_time="35.0493164" end_time="35.4354172" area="2.7281279E+10" background="5.628283E+08" height="3.52192384E+09" fwhm="0.121526681" truncated="false" identified="false" rank="1" points_across="22" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="0.6" retention_time="35.106205" start_time="34.9177055" end_time="35.2802048" area="3.0753026E+10" background="453765728" height="4.06130842E+09" fwhm="0.127620757" truncated="false" identified="false" rank="1" points_across="16" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="0" retention_time="36.74687" start_time="36.5356674" end_time="37.14047" area="6.29083341E+10" background="1.27305818E+09" height="6.11399168E+09" fwhm="0.155007869" truncated="false" identified="false" rank="1" points_across="99" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.8" retention_time="37.3439026" start_time="37.1699028" end_time="37.5295029" area="1.22578319E+10" background="100888632" height="1.65638374E+09" fwhm="0.120423138" truncated="false" identified="false" rank="1" points_across="47" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="0.5" retention_time="36.6634254" start_time="36.4654274" end_time="36.879425" area="3.732465E+10" background="6.698111E+08" height="4.489522E+09" fwhm="0.124824181" truncated="false" identified="false" rank="1" points_across="31" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.5" retention_time="41.3960762" start_time="41.0000763" end_time="41.7488747" area="8.539832E+10" background="2.38477952E+09" height="5.24950272E+09" fwhm="0.254325867" truncated="false" identified="false" rank="1" points_across="48" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="-0.1" retention_time="41.8727036" start_time="41.4083023" end_time="42.3803024" area="8.598637E+10" background="153875536" height="4.389071E+09" fwhm="0.264139146" truncated="false" identified="false" rank="1" points_across="55" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="1" isotope_dist_rank="2" isotope_dist_proportion="0.294893026">
          <precursor_mz>586.800329</precursor_mz>
          <product_mz>587.301821</product_mz>
          <collision_energy>21.898663</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-0.7" retention_time="35.2275162" start_time="35.0493164" end_time="35.4354172" area="1.62162586E+10" background="342324224" height="2.07850931E+09" fwhm="0.12223842" truncated="false" identified="false" rank="2" points_across="22" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-0.5" retention_time="35.106205" start_time="34.9177055" end_time="35.2802048" area="1.77293824E+10" background="2.767306E+08" height="2.439631E+09" fwhm="0.118319526" truncated="false" identified="false" rank="2" points_across="16" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-1" retention_time="36.7324677" start_time="36.5356674" end_time="37.14047" area="3.53117757E+10" background="9.125772E+08" height="3.537122E+09" fwhm="0.150265217" truncated="false" identified="false" rank="2" points_across="99" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-1" retention_time="37.3439026" start_time="37.1699028" end_time="37.5295029" area="7.210956E+09" background="106209432" height="980109952" fwhm="0.120295733" truncated="false" identified="false" rank="2" points_across="47" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.2" retention_time="36.67243" start_time="36.4654274" end_time="36.879425" area="1.92310784E+10" background="441051648" height="2.15966285E+09" fwhm="0.142843887" truncated="false" identified="false" rank="2" points_across="31" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.4" retention_time="41.3960762" start_time="41.0000763" end_time="41.7488747" area="4.01232036E+10" background="1.24246246E+09" height="2.416441E+09" fwhm="0.2594778" truncated="false" identified="false" rank="2" points_across="48" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.3" retention_time="41.8547058" start_time="41.4083023" end_time="42.3803024" area="3.94893967E+10" background="185592960" height="2.02102067E+09" fwhm="0.263509661" truncated="false" identified="false" rank="2" points_across="55" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="2" isotope_dist_rank="3" isotope_dist_proportion="0.09679163">
          <precursor_mz>586.800329</precursor_mz>
          <product_mz>587.803127</product_mz>
          <collision_energy>21.898663</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-1.5" retention_time="35.2374153" start_time="35.0493164" end_time="35.4354172" area="5.10375834E+09" background="1.48963E+08" height="6.592037E+08" fwhm="0.120327957" truncated="false" identified="false" rank="3" points_across="22" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-0.9" retention_time="35.0772057" start_time="34.9177055" end_time="35.2802048" area="5.473897E+09" background="169821088" height="6.883854E+08" fwhm="0.127749979" truncated="false" identified="false" rank="3" points_across="16" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-1" retention_time="36.7180672" start_time="36.5356674" end_time="37.14047" area="1.14516531E+10" background="480876672" height="1.153946E+09" fwhm="0.15783143" truncated="false" identified="false" rank="3" points_across="99" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-1.4" retention_time="37.3439026" start_time="37.1699028" end_time="37.5295029" area="2.38177126E+09" background="38068428" height="319741568" fwhm="0.121418312" truncated="false" identified="false" rank="3" points_across="47" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="0.4" retention_time="36.6634254" start_time="36.4654274" end_time="36.879425" area="5.634304E+09" background="188676960" height="6.446079E+08" fwhm="0.137393162" truncated="false" identified="false" rank="3" points_across="31" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="-0.3" retention_time="41.3960762" start_time="41.0000763" end_time="41.7488747" area="1.30447329E+10" background="340899328" height="8.127439E+08" fwhm="0.255456477" truncated="false" identified="false" rank="3" points_across="48" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="-0.2" retention_time="41.8547058" start_time="41.4083023" end_time="42.3803024" area="1.25699942E+10" background="682014656" height="658715968" fwhm="0.245291531" truncated="false" identified="false" rank="3" points_across="55" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="3" isotope_dist_rank="5" isotope_dist_proportion="0.0235912874">
          <precursor_mz>586.800329</precursor_mz>
          <product_mz>588.304402</product_mz>
          <collision_energy>21.898663</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-1.5" retention_time="35.2275162" start_time="35.0493164" end_time="35.4354172" area="1.22907866E+09" background="33401224" height="160577632" fwhm="0.120587759" truncated="false" identified="false" rank="4" points_across="22" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-1.3" retention_time="35.0917053" start_time="34.9177055" end_time="35.2802048" area="1.31713408E+09" background="53337644" height="182817280" fwhm="0.119613379" truncated="false" identified="false" rank="4" points_across="16" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-0.8" retention_time="36.7324677" start_time="36.5356674" end_time="37.14047" area="2.720432E+09" background="131824376" height="278498048" fwhm="0.154630616" truncated="false" identified="false" rank="4" points_across="99" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-1.2" retention_time="37.3439026" start_time="37.1699028" end_time="37.5295029" area="5.525198E+08" background="12345489" height="7.287325E+07" fwhm="0.127261952" truncated="false" identified="false" rank="4" points_across="47" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="0.4" retention_time="36.6634254" start_time="36.4654274" end_time="36.879425" area="1.32950592E+09" background="4.441136E+07" height="155306016" fwhm="0.1386037" truncated="false" identified="false" rank="4" points_across="31" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.3" retention_time="41.3744736" start_time="41.0000763" end_time="41.7488747" area="2.80043238E+09" background="5.093526E+08" height="182127248" fwhm="0.2443895" truncated="false" identified="false" rank="4" points_across="48" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.3" retention_time="41.8727036" start_time="41.4083023" end_time="42.3803024" area="2.64311014E+09" background="528261824" height="141361568" fwhm="0.2611148" truncated="false" identified="false" rank="4" points_across="55" user_set="FALSE" />
          </transition_results>
        </transition>
      </precursor>
    </peptide>
    <peptide auto_manage_children="false" standard_type="iRT" sequence="ELGQSGVDTYLQTK" modified_sequence="ELGQSGVDTYLQTK" calc_neutral_pep_mass="1537.762402" num_missed_cleavages="0" rt_calculator_score="54.068129989081754" predicted_retention_time="43.16" avg_measured_retention_time="42.8387">
      <implicit_modifications>
        <implicit_heavy_modifications>
          <implicit_modification index_aa="13" modification_name="Label:13C(6)15N(2) (C-term K)" mass_diff="+8" />
        </implicit_heavy_modifications>
      </implicit_modifications>
      <peptide_results>
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="40.6131172" predicted_retention_time="40.17" />
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="40.6452026" predicted_retention_time="40.09" />
        <peptide_result replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="41.8204651" predicted_retention_time="42.52" />
        <peptide_result replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="42.4131" predicted_retention_time="42.56" />
        <peptide_result replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="42.1894264" predicted_retention_time="42.08" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="45.8744736" predicted_retention_time="47.07" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="46.3151054" predicted_retention_time="47.67" />
      </peptide_results>
      <precursor charge="2" isotope_label="heavy" calc_neutral_mass="1545.776601" precursor_mz="773.895577" collision_energy="28.669985" modified_sequence="ELGQSGVDTYLQTK[+8.0]">
        <precursor_results>
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="40.6131172" start_time="40.4448166" end_time="40.7715149" fwhm="0.12815471" area="4.51977626E+10" background="633603648" height="2.69583462E+09" mass_error_ppm="-0.186570257" truncated="0" identified="false" isotope_dotp="0.99141407" user_set="FALSE" />
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="40.6452026" start_time="40.4567032" end_time="40.7902031" fwhm="0.123549812" area="4.73777971E+10" background="482509664" height="3.28206464E+09" mass_error_ppm="0.246142432" truncated="0" identified="false" isotope_dotp="0.988180637" user_set="FALSE" />
          <precursor_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="41.8204651" start_time="41.6620674" end_time="42.113266" fwhm="0.115794286" area="1.0745371E+11" background="1.30455219E+09" height="6.927113E+09" mass_error_ppm="0.6680597" truncated="0" identified="false" isotope_dotp="0.9851761" user_set="FALSE" />
          <precursor_peak replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="42.4131" start_time="42.2275" end_time="42.5755" fwhm="0.126667157" area="2.16746844E+10" background="234961360" height="1.3205824E+09" mass_error_ppm="-0.124668114" truncated="0" identified="false" isotope_dotp="0.9880806" user_set="FALSE" />
          <precursor_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="42.1894264" start_time="42.018425" end_time="42.3244247" fwhm="0.112937987" area="5.32181361E+10" background="375670240" height="3.71340851E+09" mass_error_ppm="0.3247001" truncated="0" identified="false" isotope_dotp="0.98279804" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="45.8744736" start_time="45.5864754" end_time="46.2488747" fwhm="0.339640379" area="1.43224291E+11" background="1.17922342E+09" height="3.46643E+09" mass_error_ppm="0.97674" truncated="0" identified="false" isotope_dotp="0.984405041" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="46.3151054" start_time="46.0091057" end_time="46.6283035" fwhm="0.314725071" area="1.196097E+11" background="1.09297011E+09" height="3.330553E+09" mass_error_ppm="0.0111869341" truncated="0" identified="false" isotope_dotp="0.979088068" user_set="FALSE" />
        </precursor_results>
        <transition fragment_type="precursor" isotope_dist_rank="1" isotope_dist_proportion="0.4514156">
          <precursor_mz>773.895577</precursor_mz>
          <product_mz>773.895577</product_mz>
          <collision_energy>28.669985</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="0.1" retention_time="40.6131172" start_time="40.4448166" end_time="40.7715149" area="2.09312481E+10" background="311207072" height="2.69583462E+09" fwhm="0.123538651" truncated="false" identified="false" rank="1" points_across="13" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="1.1" retention_time="40.6452026" start_time="40.4567032" end_time="40.7902031" area="2.27618427E+10" background="194930720" height="3.28206464E+09" fwhm="0.10920237" truncated="false" identified="false" rank="1" points_across="13" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="1.6" retention_time="41.8204651" start_time="41.6620674" end_time="42.113266" area="5.207761E+10" background="720119744" height="6.927113E+09" fwhm="0.110510357" truncated="false" identified="false" rank="1" points_across="76" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.3" retention_time="42.4131" start_time="42.2275" end_time="42.5755" area="1.01377352E+10" background="9.419789E+07" height="1.3205824E+09" fwhm="0.120338045" truncated="false" identified="false" rank="1" points_across="53" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="0.4" retention_time="42.1894264" start_time="42.018425" end_time="42.3244247" area="2.57697075E+10" background="174162848" height="3.71340851E+09" fwhm="0.107651986" truncated="false" identified="false" rank="1" points_across="14" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="1.2" retention_time="45.8744736" start_time="45.5864754" end_time="46.2488747" area="6.791815E+10" background="4.46972E+08" height="3.46643E+09" fwhm="0.326048434" truncated="false" identified="false" rank="1" points_across="48" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.4" retention_time="46.3151054" start_time="46.0091057" end_time="46.6283035" area="5.953886E+10" background="493244448" height="3.330553E+09" fwhm="0.314725071" truncated="false" identified="false" rank="1" points_across="31" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="1" isotope_dist_rank="2" isotope_dist_proportion="0.328991115">
          <precursor_mz>773.895577</precursor_mz>
          <product_mz>774.397062</product_mz>
          <collision_energy>28.669985</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-0.1" retention_time="40.6131172" start_time="40.4448166" end_time="40.7715149" area="1.51645972E+10" background="226256032" height="1.95036582E+09" fwhm="0.12312296" truncated="false" identified="false" rank="2" points_across="13" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-0.3" retention_time="40.6452026" start_time="40.4567032" end_time="40.7902031" area="1.54679163E+10" background="183219440" height="2.01223949E+09" fwhm="0.120631121" truncated="false" identified="false" rank="2" points_across="13" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-0.3" retention_time="41.8300667" start_time="41.6620674" end_time="42.113266" area="3.58520627E+10" background="425915264" height="4.52827955E+09" fwhm="0.115794286" truncated="false" identified="false" rank="2" points_across="76" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="0.4" retention_time="42.4131" start_time="42.2275" end_time="42.5755" area="7.1124265E+09" background="106707568" height="9.227974E+08" fwhm="0.122434527" truncated="false" identified="false" rank="2" points_across="53" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="0.4" retention_time="42.1894264" start_time="42.018425" end_time="42.3244247" area="1.81474365E+10" background="109906848" height="2.644723E+09" fwhm="0.108179487" truncated="false" identified="false" rank="2" points_across="14" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="1.1" retention_time="45.8636742" start_time="45.5864754" end_time="46.2488747" area="5.022203E+10" background="448094368" height="2.54600422E+09" fwhm="0.332255363" truncated="false" identified="false" rank="2" points_across="48" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="-0.8" retention_time="46.3115044" start_time="46.0091057" end_time="46.6283035" area="3.860101E+10" background="372341760" height="2.384703E+09" fwhm="0.298576057" truncated="false" identified="false" rank="2" points_across="31" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="2" isotope_dist_rank="3" isotope_dist_proportion="0.141858578">
          <precursor_mz>773.895577</precursor_mz>
          <product_mz>774.898401</product_mz>
          <collision_energy>28.669985</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-0.9" retention_time="40.6131172" start_time="40.4448166" end_time="40.7715149" area="7.058174E+09" background="9.253933E+07" height="9.089212E+08" fwhm="0.124848545" truncated="false" identified="false" rank="3" points_across="13" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-0.8" retention_time="40.6452026" start_time="40.4567032" end_time="40.7902031" area="7.12299E+09" background="9.311614E+07" height="935324352" fwhm="0.117880568" truncated="false" identified="false" rank="3" points_across="13" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="0.2" retention_time="41.8204651" start_time="41.6620674" end_time="42.113266" area="1.43153019E+10" background="158517248" height="1.84720909E+09" fwhm="0.11504212" truncated="false" identified="false" rank="3" points_across="76" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.7" retention_time="42.4131" start_time="42.2275" end_time="42.5755" area="3.43889971E+09" background="3.092607E+07" height="4.375321E+08" fwhm="0.126667157" truncated="false" identified="false" rank="3" points_across="53" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="0.1" retention_time="42.1804276" start_time="42.018425" end_time="42.3244247" area="6.86694246E+09" background="52725944" height="9.849925E+08" fwhm="0.11004772" truncated="false" identified="false" rank="3" points_across="14" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.1" retention_time="45.9644737" start_time="45.5864754" end_time="46.2488747" area="1.86995589E+10" background="169478512" height="9.472844E+08" fwhm="0.339640379" truncated="false" identified="false" rank="3" points_across="48" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.6" retention_time="46.3115044" start_time="46.0091057" end_time="46.6283035" area="1.58716252E+10" background="227383888" height="906705344" fwhm="0.308306932" truncated="false" identified="false" rank="3" points_across="31" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="3" isotope_dist_rank="4" isotope_dist_proportion="0.0450559035">
          <precursor_mz>773.895577</precursor_mz>
          <product_mz>775.399698</product_mz>
          <collision_energy>28.669985</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-1.3" retention_time="40.6131172" start_time="40.4448166" end_time="40.7715149" area="2.04374387E+09" background="3601217.75" height="254779264" fwhm="0.12815471" truncated="false" identified="false" rank="4" points_across="13" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-1.5" retention_time="40.6452026" start_time="40.4567032" end_time="40.7902031" area="2.02504922E+09" background="11243361" height="256567984" fwhm="0.123549812" truncated="false" identified="false" rank="4" points_across="13" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-0.7" retention_time="41.8108673" start_time="41.6620674" end_time="42.113266" area="5.20874035E+09" background="0" height="6.490078E+08" fwhm="0.1141517" truncated="false" identified="false" rank="4" points_across="76" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.1" retention_time="42.4131" start_time="42.2275" end_time="42.5755" area="9.856253E+08" background="3129842" height="127096016" fwhm="0.125307277" truncated="false" identified="false" rank="4" points_across="53" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.4" retention_time="42.1804276" start_time="42.018425" end_time="42.3244247" area="2.43404877E+09" background="3.887458E+07" height="3.391053E+08" fwhm="0.112937987" truncated="false" identified="false" rank="4" points_across="14" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.2" retention_time="45.8744736" start_time="45.5864754" end_time="46.2488747" area="6.384552E+09" background="114678520" height="344248480" fwhm="0.3306588" truncated="false" identified="false" rank="4" points_across="48" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="-0.2" retention_time="46.3115044" start_time="46.0091057" end_time="46.6283035" area="5.59820954E+09" background="0" height="321884864" fwhm="0.308050483" truncated="false" identified="false" rank="4" points_across="31" user_set="FALSE" />
          </transition_results>
        </transition>
      </precursor>
    </peptide>
    <peptide auto_manage_children="false" standard_type="iRT" sequence="GILFVGSGVSGGEEGAR" modified_sequence="GILFVGSGVSGGEEGAR" calc_neutral_pep_mass="1590.800185" num_missed_cleavages="0" rt_calculator_score="67.908910861294515" predicted_retention_time="49.17" avg_measured_retention_time="49.0965576">
      <implicit_modifications>
        <implicit_heavy_modifications>
          <implicit_modification index_aa="16" modification_name="Label:13C(6)15N(4) (C-term R)" mass_diff="+10" />
        </implicit_heavy_modifications>
      </implicit_modifications>
      <peptide_results>
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="45.82052" predicted_retention_time="45.81" />
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="45.8072052" predicted_retention_time="45.74" />
        <peptide_result replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="48.4588661" predicted_retention_time="48.5" />
        <peptide_result replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="48.5553" predicted_retention_time="48.22" />
        <peptide_result replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="47.7964249" predicted_retention_time="47.94" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="53.2364769" predicted_retention_time="53.62" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="54.0011024" predicted_retention_time="54.33" />
      </peptide_results>
      <precursor charge="2" isotope_label="heavy" calc_neutral_mass="1600.808454" precursor_mz="801.411503" collision_energy="29.624665" modified_sequence="GILFVGSGVSGGEEGAR[+10.0]">
        <precursor_results>
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="45.82052" start_time="45.553215" end_time="46.0185165" fwhm="0.124509826" area="3.96440125E+10" background="574011648" height="2.43417472E+09" mass_error_ppm="0.209348753" truncated="0" identified="false" isotope_dotp="0.9817782" user_set="FALSE" />
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="45.8072052" start_time="45.5607033" end_time="45.981205" fwhm="0.119144335" area="4.09040323E+10" background="6.583194E+08" height="2.55870566E+09" mass_error_ppm="0.0278587788" truncated="0" identified="false" isotope_dotp="0.9785162" user_set="FALSE" />
          <precursor_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="48.4588661" start_time="48.2140656" end_time="48.7372665" fwhm="0.114036076" area="7.26346056E+10" background="1.22287552E+09" height="4.26865382E+09" mass_error_ppm="0.9499348" truncated="0" identified="false" isotope_dotp="0.9887919" user_set="FALSE" />
          <precursor_peak replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="48.5553" start_time="48.2769" end_time="48.7641029" fwhm="0.127127469" area="1.86330747E+10" background="292560736" height="1.00440518E+09" mass_error_ppm="-0.371802181" truncated="0" identified="false" isotope_dotp="0.994176745" user_set="FALSE" />
          <precursor_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="47.7964249" start_time="47.6614265" end_time="48.0034256" fwhm="0.101265855" area="3.68489677E+10" background="3.00183552E+09" height="2.58130637E+09" mass_error_ppm="0.102779113" truncated="0" identified="false" isotope_dotp="0.9882336" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="53.2364769" start_time="52.9304771" end_time="53.5028763" fwhm="0.241195545" area="9.193374E+10" background="1.17274893E+09" height="3.05425152E+09" mass_error_ppm="0.8646614" truncated="0" identified="false" isotope_dotp="0.9916358" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="54.0011024" start_time="53.8319054" end_time="54.2783051" fwhm="0.1858139" area="7.81834158E+10" background="1.8809015E+09" height="3.391074E+09" mass_error_ppm="0.253306478" truncated="0" identified="false" isotope_dotp="0.974299252" user_set="FALSE" />
        </precursor_results>
        <transition fragment_type="precursor" isotope_dist_rank="1" isotope_dist_proportion="0.4403066">
          <precursor_mz>801.411503</precursor_mz>
          <product_mz>801.411503</product_mz>
          <collision_energy>29.624665</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="0.9" retention_time="45.82052" start_time="45.553215" end_time="46.0185165" area="1.922104E+10" background="257747088" height="2.43417472E+09" fwhm="0.118646868" truncated="false" identified="false" rank="1" points_across="15" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="0.5" retention_time="45.8072052" start_time="45.5607033" end_time="45.981205" area="2.00400548E+10" background="288285280" height="2.55870566E+09" fwhm="0.115593761" truncated="false" identified="false" rank="1" points_across="12" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="1.1" retention_time="48.4588661" start_time="48.2140656" end_time="48.7372665" area="3.40597842E+10" background="6.403946E+08" height="4.26865382E+09" fwhm="0.104899682" truncated="false" identified="false" rank="1" points_across="98" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="0.1" retention_time="48.5553" start_time="48.2769" end_time="48.7641029" area="8.4262784E+09" background="1.358593E+08" height="1.00440518E+09" fwhm="0.120621324" truncated="false" identified="false" rank="1" points_across="55" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="0.1" retention_time="47.7964249" start_time="47.6614265" end_time="48.0034256" area="1.62037187E+10" background="1.34215347E+09" height="2.58130637E+09" fwhm="0.09767378" truncated="false" identified="false" rank="1" points_across="12" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="1.7" retention_time="53.2364769" start_time="52.9304771" end_time="53.5028763" area="4.30414971E+10" background="406843360" height="3.05425152E+09" fwhm="0.233129814" truncated="false" identified="false" rank="1" points_across="49" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.1" retention_time="54.0011024" start_time="53.8319054" end_time="54.2783051" area="3.88780032E+10" background="571852736" height="3.391074E+09" fwhm="0.1858139" truncated="false" identified="false" rank="1" points_across="26" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="1" isotope_dist_rank="2" isotope_dist_proportion="0.33077082">
          <precursor_mz>801.411503</precursor_mz>
          <product_mz>801.912993</product_mz>
          <collision_energy>29.624665</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="0" retention_time="45.82052" start_time="45.553215" end_time="46.0185165" area="1.29019617E+10" background="211978560" height="1.61645658E+09" fwhm="0.122096941" truncated="false" identified="false" rank="2" points_across="15" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="0" retention_time="45.8072052" start_time="45.5607033" end_time="45.981205" area="1.31292805E+10" background="235112288" height="1.647604E+09" fwhm="0.117650472" truncated="false" identified="false" rank="2" points_across="12" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="1" retention_time="48.4684677" start_time="48.2140656" end_time="48.7372665" area="2.50944778E+10" background="398802944" height="3.13148314E+09" fwhm="0.114036076" truncated="false" identified="false" rank="2" points_across="98" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.8" retention_time="48.5785027" start_time="48.2769" end_time="48.7641029" area="6.36233933E+09" background="7.808699E+07" height="7.667044E+08" fwhm="0.1259242" truncated="false" identified="false" rank="2" points_across="55" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="0.3" retention_time="47.7964249" start_time="47.6614265" end_time="48.0034256" area="1.31328471E+10" background="1.03709594E+09" height="2.12213491E+09" fwhm="0.09764961" truncated="false" identified="false" rank="2" points_across="12" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.2" retention_time="53.211277" start_time="52.9304771" end_time="53.5028763" area="3.08320358E+10" background="559054144" height="2.08685286E+09" fwhm="0.233179" truncated="false" identified="false" rank="2" points_across="49" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.8" retention_time="54.0011024" start_time="53.8319054" end_time="54.2783051" area="2.51134566E+10" background="819304448" height="2.185631E+09" fwhm="0.185364246" truncated="false" identified="false" rank="2" points_across="26" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="2" isotope_dist_rank="3" isotope_dist_proportion="0.145006523">
          <precursor_mz>801.411503</precursor_mz>
          <product_mz>802.414334</product_mz>
          <collision_energy>29.624665</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-1.3" retention_time="45.82052" start_time="45.553215" end_time="46.0185165" area="5.57650074E+09" background="6.268427E+07" height="6.855385E+08" fwhm="0.124509826" truncated="false" identified="false" rank="3" points_across="15" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-1.2" retention_time="45.8072052" start_time="45.5607033" end_time="45.981205" area="5.728953E+09" background="111965208" height="7.14718E+08" fwhm="0.119144335" truncated="false" identified="false" rank="3" points_across="12" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="0.6" retention_time="48.463665" start_time="48.2140656" end_time="48.7372665" area="1.01797243E+10" background="159801232" height="1.31298445E+09" fwhm="0.106304377" truncated="false" identified="false" rank="3" points_across="98" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.6" retention_time="48.5553" start_time="48.2769" end_time="48.7641029" area="2.909705E+09" background="52227196" height="336989664" fwhm="0.127127469" truncated="false" identified="false" rank="3" points_across="55" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.4" retention_time="47.7964249" start_time="47.6614265" end_time="48.0034256" area="5.752346E+09" background="469736832" height="924396864" fwhm="0.09668733" truncated="false" identified="false" rank="3" points_across="12" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="-0.3" retention_time="53.211277" start_time="52.9304771" end_time="53.5028763" area="1.3416318E+10" background="206851424" height="9.00397E+08" fwhm="0.240461349" truncated="false" identified="false" rank="3" points_across="49" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="-0.2" retention_time="54.0011024" start_time="53.8319054" end_time="54.2783051" area="1.0852437E+10" background="346470560" height="9.587732E+08" fwhm="0.185771763" truncated="false" identified="false" rank="3" points_across="26" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="3" isotope_dist_rank="4" isotope_dist_proportion="0.0465909541">
          <precursor_mz>801.411503</precursor_mz>
          <product_mz>802.915634</product_mz>
          <collision_energy>29.624665</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-0.9" retention_time="45.82052" start_time="45.553215" end_time="46.0185165" area="1.944511E+09" background="41601724" height="248951744" fwhm="0.12343394" truncated="false" identified="false" rank="4" points_across="15" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-1" retention_time="45.8072052" start_time="45.5607033" end_time="45.981205" area="2.00574669E+09" background="2.29567E+07" height="2.572161E+08" fwhm="0.110659063" truncated="false" identified="false" rank="4" points_across="12" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="0.1" retention_time="48.463665" start_time="48.2140656" end_time="48.7372665" area="3.30062157E+09" background="23876796" height="3.925726E+08" fwhm="0.111904405" truncated="false" identified="false" rank="4" points_across="98" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-1" retention_time="48.5495" start_time="48.2769" end_time="48.7641029" area="9.347515E+08" background="26387246" height="109860960" fwhm="0.123979151" truncated="false" identified="false" rank="4" points_across="55" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="0.3" retention_time="47.787426" start_time="47.6614265" end_time="48.0034256" area="1.76005478E+09" background="152849344" height="262634944" fwhm="0.101265855" truncated="false" identified="false" rank="4" points_across="12" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.9" retention_time="53.1896744" start_time="52.9304771" end_time="53.5028763" area="4.643893E+09" background="0" height="309961344" fwhm="0.241195545" truncated="false" identified="false" rank="4" points_across="49" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="-0.6" retention_time="54.0011024" start_time="53.8319054" end_time="54.2783051" area="3.33951923E+09" background="143273744" height="304396640" fwhm="0.174108773" truncated="false" identified="false" rank="4" points_across="26" user_set="FALSE" />
          </transition_results>
        </transition>
      </precursor>
    </peptide>
    <peptide auto_manage_children="false" standard_type="iRT" sequence="GLILVGGYGTR" modified_sequence="GLILVGGYGTR" calc_neutral_pep_mass="1104.629144" num_missed_cleavages="0" rt_calculator_score="64.953027733913061" predicted_retention_time="47.88" avg_measured_retention_time="47.76317">
      <implicit_modifications>
        <implicit_heavy_modifications>
          <implicit_modification index_aa="10" modification_name="Label:13C(6)15N(4) (C-term R)" mass_diff="+10" />
        </implicit_heavy_modifications>
      </implicit_modifications>
      <peptide_results>
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="44.3058167" predicted_retention_time="44.6" />
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="44.2702026" predicted_retention_time="44.53" />
        <peptide_result replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="47.1724663" predicted_retention_time="47.22" />
        <peptide_result replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="47.2677" predicted_retention_time="47.01" />
        <peptide_result replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="46.3024254" predicted_retention_time="46.69" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="52.1312752" predicted_retention_time="52.23" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="52.8923035" predicted_retention_time="52.9" />
      </peptide_results>
      <precursor charge="2" isotope_label="heavy" calc_neutral_mass="1114.637413" precursor_mz="558.325982" collision_energy="20.873587" modified_sequence="GLILVGGYGTR[+10.0]">
        <precursor_results>
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="44.3058167" start_time="44.0979156" end_time="44.50382" fwhm="0.132568061" area="4.54361252E+10" background="7.509227E+08" height="3.41728486E+09" mass_error_ppm="0.173695967" truncated="0" identified="false" isotope_dotp="0.991235852" user_set="FALSE" />
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="44.2702026" start_time="44.0672035" end_time="44.4442024" fwhm="0.136658281" area="4.744237E+10" background="799154560" height="3.57263846E+09" mass_error_ppm="0.0365045443" truncated="0" identified="false" isotope_dotp="0.9909396" user_set="FALSE" />
          <precursor_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="47.1724663" start_time="47.0428658" end_time="47.4220657" fwhm="0.107781775" area="7.173474E+10" background="1.90881792E+09" height="6.34415053E+09" mass_error_ppm="-0.5845625" truncated="0" identified="false" isotope_dotp="0.9931055" user_set="FALSE" />
          <precursor_peak replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="47.2677" start_time="47.0937" end_time="47.3953" fwhm="0.107331358" area="2.10438328E+10" background="619159552" height="2.11476544E+09" mass_error_ppm="-0.6288152" truncated="0" identified="false" isotope_dotp="0.98295325" user_set="FALSE" />
          <precursor_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="46.3024254" start_time="46.1314278" end_time="46.5094261" fwhm="0.102265492" area="5.289459E+10" background="1.03274605E+09" height="4.912097E+09" mass_error_ppm="0.177833512" truncated="0" identified="false" isotope_dotp="0.991451561" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="52.1312752" start_time="51.8720741" end_time="52.7576752" fwhm="0.278643668" area="1.117E+11" background="1.69081856E+09" height="3.65565E+09" mass_error_ppm="0.7293424" truncated="0" identified="false" isotope_dotp="0.980983853" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="52.8923035" start_time="52.5755043" end_time="53.3279037" fwhm="0.366317868" area="1.35876207E+11" background="1.88256282E+09" height="3.72845158E+09" mass_error_ppm="-0.035073448" truncated="0" identified="false" isotope_dotp="0.981546342" user_set="FALSE" />
        </precursor_results>
        <transition fragment_type="precursor" isotope_dist_rank="1" isotope_dist_proportion="0.5568586">
          <precursor_mz>558.325982</precursor_mz>
          <product_mz>558.325982</product_mz>
          <collision_energy>20.873587</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="0.6" retention_time="44.3058167" start_time="44.0979156" end_time="44.50382" area="2.6542592E+10" background="487320768" height="3.41728486E+09" fwhm="0.124026284" truncated="false" identified="false" rank="1" points_across="19" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="0.5" retention_time="44.2702026" start_time="44.0672035" end_time="44.4442024" area="2.77070664E+10" background="467837632" height="3.57263846E+09" fwhm="0.122855984" truncated="false" identified="false" rank="1" points_across="16" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="0" retention_time="47.1724663" start_time="47.0428658" end_time="47.4220657" area="4.19500155E+10" background="1.13229786E+09" height="6.34415053E+09" fwhm="0.09781757" truncated="false" identified="false" rank="1" points_across="60" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.5" retention_time="47.2677" start_time="47.0937" end_time="47.3953" area="1.25293619E+10" background="351097152" height="2.11476544E+09" fwhm="0.09319711" truncated="false" identified="false" rank="1" points_across="36" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="0.6" retention_time="46.3024254" start_time="46.1314278" end_time="46.5094261" area="3.1106902E+10" background="5.78647E+08" height="4.912097E+09" fwhm="0.09256139" truncated="false" identified="false" rank="1" points_across="19" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.9" retention_time="52.1312752" start_time="51.8720741" end_time="52.7576752" area="6.290951E+10" background="1.20742093E+09" height="3.65565E+09" fwhm="0.272879839" truncated="false" identified="false" rank="1" points_across="55" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.4" retention_time="52.8923035" start_time="52.5755043" end_time="53.3279037" area="7.707632E+10" background="1.09817178E+09" height="3.72845158E+09" fwhm="0.366317868" truncated="false" identified="false" rank="1" points_across="63" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="1" isotope_dist_rank="2" isotope_dist_proportion="0.29546234">
          <precursor_mz>558.325982</precursor_mz>
          <product_mz>558.827496</product_mz>
          <collision_energy>20.873587</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-0.5" retention_time="44.28602" start_time="44.0979156" end_time="44.50382" area="1.38785352E+10" background="244346864" height="1.6596151E+09" fwhm="0.132568061" truncated="false" identified="false" rank="2" points_across="19" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-0.6" retention_time="44.2702026" start_time="44.0672035" end_time="44.4442024" area="1.42472858E+10" background="3.072069E+08" height="1.64760832E+09" fwhm="0.136658281" truncated="false" identified="false" rank="2" points_across="16" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-1.4" retention_time="47.1724663" start_time="47.0428658" end_time="47.4220657" area="2.12160983E+10" background="5.830946E+08" height="2.95456742E+09" fwhm="0.10639818" truncated="false" identified="false" rank="2" points_across="60" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-1.1" retention_time="47.2677" start_time="47.0937" end_time="47.3953" area="5.94046157E+09" background="205034752" height="9.653545E+08" fwhm="0.100160822" truncated="false" identified="false" rank="2" points_across="36" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.5" retention_time="46.3024254" start_time="46.1314278" end_time="46.5094261" area="1.55896412E+10" background="348831264" height="2.29265459E+09" fwhm="0.101318583" truncated="false" identified="false" rank="2" points_across="19" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.4" retention_time="52.1312752" start_time="51.8720741" end_time="52.7576752" area="3.68680673E+10" background="4.588462E+08" height="2.1304809E+09" fwhm="0.27673763" truncated="false" identified="false" rank="2" points_across="55" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="-0.8" retention_time="52.8923035" start_time="52.5755043" end_time="53.3279037" area="4.44952166E+10" background="6.810278E+08" height="2.1743017E+09" fwhm="0.3618974" truncated="false" identified="false" rank="2" points_across="63" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="2" isotope_dist_rank="3" isotope_dist_proportion="0.09315352">
          <precursor_mz>558.325982</precursor_mz>
          <product_mz>559.328838</product_mz>
          <collision_energy>20.873587</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-0.2" retention_time="44.3058167" start_time="44.0979156" end_time="44.50382" area="4.102835E+09" background="0" height="506550848" fwhm="0.1279889" truncated="false" identified="false" rank="3" points_across="19" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-0.7" retention_time="44.2702026" start_time="44.0672035" end_time="44.4442024" area="4.59364147E+09" background="0" height="536651456" fwhm="0.134507746" truncated="false" identified="false" rank="3" points_across="16" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-1.6" retention_time="47.1676674" start_time="47.0428658" end_time="47.4220657" area="6.925403E+09" background="161102832" height="9.853136E+08" fwhm="0.107781775" truncated="false" identified="false" rank="3" points_across="60" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.5" retention_time="47.2735023" start_time="47.0937" end_time="47.3953" area="2.040563E+09" background="21918804" height="315417664" fwhm="0.107331358" truncated="false" identified="false" rank="3" points_across="36" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.2" retention_time="46.3024254" start_time="46.1314278" end_time="46.5094261" area="5.0816425E+09" background="1.052678E+08" height="7.837967E+08" fwhm="0.09695537" truncated="false" identified="false" rank="3" points_across="19" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.9" retention_time="52.1384773" start_time="51.8720741" end_time="52.7576752" area="9.827692E+09" background="0" height="5.642324E+08" fwhm="0.278643668" truncated="false" identified="false" rank="3" points_across="55" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0" retention_time="52.8491058" start_time="52.5755043" end_time="53.3279037" area="1.17745644E+10" background="103363336" height="605394560" fwhm="0.336746156" truncated="false" identified="false" rank="3" points_across="63" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="3" isotope_dist_rank="5" isotope_dist_proportion="0.0216344576">
          <precursor_mz>558.325982</precursor_mz>
          <product_mz>559.830138</product_mz>
          <collision_energy>20.873587</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-0.3" retention_time="44.3058167" start_time="44.0979156" end_time="44.50382" area="912163136" background="19255052" height="122996816" fwhm="0.12274006" truncated="false" identified="false" rank="4" points_across="19" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-0.4" retention_time="44.2702026" start_time="44.0672035" end_time="44.4442024" area="8.943765E+08" background="24109984" height="1.150218E+08" fwhm="0.134983867" truncated="false" identified="false" rank="4" points_across="16" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-0.7" retention_time="47.1724663" start_time="47.0428658" end_time="47.4220657" area="1.64322266E+09" background="32322554" height="227845952" fwhm="0.101731494" truncated="false" identified="false" rank="4" points_across="60" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="1.1" retention_time="47.2677" start_time="47.0937" end_time="47.3953" area="533445728" background="4.110886E+07" height="73850744" fwhm="0.09539558" truncated="false" identified="false" rank="4" points_across="36" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.4" retention_time="46.3024254" start_time="46.1314278" end_time="46.5094261" area="1.11640422E+09" background="0" height="165117392" fwhm="0.102265492" truncated="false" identified="false" rank="4" points_across="19" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.6" retention_time="52.1312752" start_time="51.8720741" end_time="52.7576752" area="2.09473178E+09" background="2.455144E+07" height="136175520" fwhm="0.264053" truncated="false" identified="false" rank="4" points_across="55" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0" retention_time="52.8995056" start_time="52.5755043" end_time="53.3279037" area="2.53011E+09" background="0" height="137008016" fwhm="0.322872877" truncated="false" identified="false" rank="4" points_across="63" user_set="FALSE" />
          </transition_results>
        </transition>
      </precursor>
    </peptide>
    <peptide auto_manage_children="false" standard_type="iRT" sequence="SFANQPLEVVYSK" modified_sequence="SFANQPLEVVYSK" calc_neutral_pep_mass="1480.756195" num_missed_cleavages="0" rt_calculator_score="66.401934853055" predicted_retention_time="48.51" avg_measured_retention_time="48.5844421">
      <implicit_modifications>
        <implicit_heavy_modifications>
          <implicit_modification index_aa="12" modification_name="Label:13C(6)15N(2) (C-term K)" mass_diff="+8" />
        </implicit_heavy_modifications>
      </implicit_modifications>
      <peptide_results>
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="45.2364159" predicted_retention_time="45.19" />
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="45.241703" predicted_retention_time="45.12" />
        <peptide_result replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="47.7772675" predicted_retention_time="47.85" />
        <peptide_result replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="47.7143021" predicted_retention_time="47.6" />
        <peptide_result replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="47.2294273" predicted_retention_time="47.3" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="53.1032753" predicted_retention_time="52.91" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="53.7887039" predicted_retention_time="53.6" />
      </peptide_results>
      <precursor charge="2" isotope_label="heavy" calc_neutral_mass="1488.770394" precursor_mz="745.392473" collision_energy="27.643873" modified_sequence="SFANQPLEVVYSK[+8.0]">
        <precursor_results>
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="45.2364159" start_time="45.0879173" end_time="45.3948174" fwhm="0.154744133" area="3.61914532E+10" background="307232064" height="2.09371635E+09" mass_error_ppm="0.007212895" truncated="0" identified="false" isotope_dotp="0.973494649" user_set="FALSE" />
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="45.241703" start_time="45.0677032" end_time="45.4012032" fwhm="0.154104948" area="3.91252173E+10" background="468009472" height="2.20166246E+09" mass_error_ppm="0.04547371" truncated="0" identified="false" isotope_dotp="0.97622174" user_set="FALSE" />
          <precursor_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="47.7772675" start_time="47.6332664" end_time="47.9308662" fwhm="0.118116409" area="7.033274E+10" background="1.35193037E+09" height="4.19755725E+09" mass_error_ppm="0.364346057" truncated="0" identified="false" isotope_dotp="0.9898843" user_set="FALSE" />
          <precursor_peak replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="47.7143021" start_time="47.5461" end_time="47.870903" fwhm="0.113589644" area="1.66795766E+10" background="6.572465E+08" height="1.19411E+09" mass_error_ppm="-0.5128462" truncated="0" identified="false" isotope_dotp="0.9772524" user_set="FALSE" />
          <precursor_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="47.2294273" start_time="47.0674248" end_time="47.4364281" fwhm="0.138684437" area="5.1660587E+10" background="5.240318E+08" height="2.98947482E+09" mass_error_ppm="-0.0106765423" truncated="0" identified="false" isotope_dotp="0.9785336" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="53.1032753" start_time="53.1032753" end_time="53.3948746" area="5.16481966E+10" background="718351552" height="2.75075533E+09" mass_error_ppm="0.8421751" truncated="0" identified="false" isotope_dotp="0.9906747" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="53.7887039" start_time="53.6411057" end_time="54.1559029" fwhm="0.255770773" area="1.15213517E+11" background="1.08639923E+09" height="3.468685E+09" mass_error_ppm="-0.0583587177" truncated="0" identified="false" isotope_dotp="0.986342132" user_set="FALSE" />
        </precursor_results>
        <transition fragment_type="precursor" isotope_dist_rank="1" isotope_dist_proportion="0.4480884">
          <precursor_mz>745.392473</precursor_mz>
          <product_mz>745.392473</product_mz>
          <collision_energy>27.643873</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="0.7" retention_time="45.2364159" start_time="45.0879173" end_time="45.3948174" area="1.60001618E+10" background="1.369469E+08" height="2.09371635E+09" fwhm="0.119666964" truncated="false" identified="false" rank="1" points_across="13" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="0.7" retention_time="45.241703" start_time="45.0677032" end_time="45.4012032" area="1.75309455E+10" background="226768864" height="2.20166246E+09" fwhm="0.1236153" truncated="false" identified="false" rank="1" points_across="16" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="0.7" retention_time="47.7772675" start_time="47.6332664" end_time="47.9308662" area="3.18704742E+10" background="603082560" height="4.19755725E+09" fwhm="0.118116409" truncated="false" identified="false" rank="1" points_across="54" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="0.1" retention_time="47.7143021" start_time="47.5461" end_time="47.870903" area="8.0865367E+09" background="3.452831E+08" height="1.19411E+09" fwhm="0.102945745" truncated="false" identified="false" rank="1" points_across="44" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="0.7" retention_time="47.2294273" start_time="47.0674248" end_time="47.4364281" area="2.48009441E+10" background="2.000727E+08" height="2.98947482E+09" fwhm="0.128457516" truncated="false" identified="false" rank="1" points_across="19" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.8" retention_time="53.1032753" start_time="53.1032753" end_time="53.3948746" area="2.35671224E+10" background="458169760" height="2.75075533E+09" fwhm="0.1531828" fwhm_degenerate="true" truncated="false" identified="false" rank="1" points_across="24" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.7" retention_time="53.7887039" start_time="53.6411057" end_time="54.1559029" area="5.215048E+10" background="414243552" height="3.468685E+09" fwhm="0.243127674" truncated="false" identified="false" rank="1" points_across="32" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="1" isotope_dist_rank="2" isotope_dist_proportion="0.333507061">
          <precursor_mz>745.392473</precursor_mz>
          <product_mz>745.893977</product_mz>
          <collision_energy>27.643873</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="0.5" retention_time="45.2364159" start_time="45.0879173" end_time="45.3948174" area="1.19899924E+10" background="9.17849E+07" height="1.53563226E+09" fwhm="0.1218324" truncated="false" identified="false" rank="2" points_across="13" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="0.5" retention_time="45.241703" start_time="45.0677032" end_time="45.4012032" area="1.28716462E+10" background="134669472" height="1.60712922E+09" fwhm="0.124050714" truncated="false" identified="false" rank="2" points_across="16" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="0.5" retention_time="47.7772675" start_time="47.6332664" end_time="47.9308662" area="2.42755973E+10" background="435917152" height="3.41158579E+09" fwhm="0.109594166" truncated="false" identified="false" rank="2" points_across="54" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-1.3" retention_time="47.7143021" start_time="47.5461" end_time="47.870903" area="5.28411648E+09" background="221180592" height="760317952" fwhm="0.105971478" truncated="false" identified="false" rank="2" points_across="44" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.6" retention_time="47.2294273" start_time="47.0674248" end_time="47.4364281" area="1.64030116E+10" background="169747584" height="1.950443E+09" fwhm="0.1301587" truncated="false" identified="false" rank="2" points_across="19" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="1" retention_time="53.1032753" start_time="53.1032753" end_time="53.3948746" area="1.80423414E+10" background="242898976" height="2.28375066E+09" fwhm="0.14774251" fwhm_degenerate="true" truncated="false" identified="false" rank="2" points_across="24" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="-0.5" retention_time="53.8247032" start_time="53.6411057" end_time="54.1559029" area="4.195385E+10" background="249819648" height="2.77799936E+09" fwhm="0.248460323" truncated="false" identified="false" rank="2" points_across="32" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="2" isotope_dist_rank="3" isotope_dist_proportion="0.142379314">
          <precursor_mz>745.392473</precursor_mz>
          <product_mz>746.395351</product_mz>
          <collision_energy>27.643873</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-1.9" retention_time="45.2364159" start_time="45.0879173" end_time="45.3948174" area="6.27043533E+09" background="7.136421E+07" height="7.330962E+08" fwhm="0.131013751" truncated="false" identified="false" rank="3" points_across="13" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-1.8" retention_time="45.241703" start_time="45.0677032" end_time="45.4012032" area="6.676644E+09" background="9.031808E+07" height="767860544" fwhm="0.132582009" truncated="false" identified="false" rank="3" points_across="16" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-0.6" retention_time="47.77247" start_time="47.6332664" end_time="47.9308662" area="1.108999E+10" background="246591152" height="1.43833165E+09" fwhm="0.117668852" truncated="false" identified="false" rank="3" points_across="54" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.2" retention_time="47.7143021" start_time="47.5461" end_time="47.870903" area="2.393137E+09" background="6.861204E+07" height="330491520" fwhm="0.10564284" truncated="false" identified="false" rank="3" points_across="44" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.5" retention_time="47.2294273" start_time="47.0674248" end_time="47.4364281" area="7.61453466E+09" background="136307136" height="823712064" fwhm="0.137585476" truncated="false" identified="false" rank="3" points_across="19" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.7" retention_time="53.1068764" start_time="53.1032753" end_time="53.3948746" area="7.90709555E+09" background="13447521" height="953753856" fwhm="0.1478234" fwhm_degenerate="true" truncated="false" identified="false" rank="3" points_across="24" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="-1.2" retention_time="53.8247032" start_time="53.6411057" end_time="54.1559029" area="1.597769E+10" background="422336064" height="1.07110144E+09" fwhm="0.239667416" truncated="false" identified="false" rank="3" points_across="32" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="3" isotope_dist_rank="4" isotope_dist_proportion="0.0442643464">
          <precursor_mz>745.392473</precursor_mz>
          <product_mz>746.896679</product_mz>
          <collision_energy>27.643873</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-2.6" retention_time="45.246315" start_time="45.0879173" end_time="45.3948174" area="1.93086093E+09" background="7136055" height="203637424" fwhm="0.154744133" truncated="false" identified="false" rank="4" points_across="13" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-2.4" retention_time="45.2707024" start_time="45.0677032" end_time="45.4012032" area="2.04598234E+09" background="16253061" height="2.126195E+08" fwhm="0.154104948" truncated="false" identified="false" rank="4" points_across="16" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-0.7" retention_time="47.7772675" start_time="47.6332664" end_time="47.9308662" area="3.09668429E+09" background="6.633957E+07" height="4.005086E+08" fwhm="0.114700012" truncated="false" identified="false" rank="4" points_across="54" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-2.2" retention_time="47.7143021" start_time="47.5461" end_time="47.870903" area="9.157871E+08" background="22170822" height="113937376" fwhm="0.113589644" truncated="false" identified="false" rank="4" points_across="44" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-1.5" retention_time="47.2204247" start_time="47.0674248" end_time="47.4364281" area="2.84209587E+09" background="17904374" height="272856256" fwhm="0.138684437" truncated="false" identified="false" rank="4" points_across="19" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.5" retention_time="53.1104736" start_time="53.1032753" end_time="53.3948746" area="2.1316384E+09" background="3835248.5" height="261925168" fwhm="0.142143965" fwhm_degenerate="true" truncated="false" identified="false" rank="4" points_across="24" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="-0.6" retention_time="53.7887039" start_time="53.6411057" end_time="54.1559029" area="5.13149338E+09" background="0" height="332942848" fwhm="0.255770773" truncated="false" identified="false" rank="4" points_across="32" user_set="FALSE" />
          </transition_results>
        </transition>
      </precursor>
    </peptide>
    <peptide auto_manage_children="false" standard_type="iRT" sequence="LTILEELR" modified_sequence="LTILEELR" calc_neutral_pep_mass="985.580797" num_missed_cleavages="0" rt_calculator_score="75.282485197019966" predicted_retention_time="52.36" avg_measured_retention_time="53.0557137">
      <implicit_modifications>
        <implicit_heavy_modifications>
          <implicit_modification index_aa="7" modification_name="Label:13C(6)15N(4) (C-term R)" mass_diff="+10" />
        </implicit_heavy_modifications>
      </implicit_modifications>
      <peptide_results>
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="48.5133171" predicted_retention_time="48.81" />
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="48.4027023" predicted_retention_time="48.75" />
        <peptide_result replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="51.97727" predicted_retention_time="51.69" />
        <peptide_result replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="51.5597" predicted_retention_time="51.24" />
        <peptide_result replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="50.8114281" predicted_retention_time="51.06" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="59.5364761" predicted_retention_time="57.11" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="60.5891037" predicted_retention_time="57.87" />
      </peptide_results>
      <precursor charge="2" isotope_label="heavy" calc_neutral_mass="995.589066" precursor_mz="498.801809" collision_energy="18.730716" modified_sequence="LTILEELR[+10.0]">
        <precursor_results>
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="48.5133171" start_time="48.3351173" end_time="48.701416" fwhm="0.114649728" area="1.57194875E+10" background="1.972409E+08" height="1.4982857E+09" mass_error_ppm="-0.0444804244" truncated="0" identified="false" isotope_dotp="0.986740768" user_set="FALSE" />
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="48.4027023" start_time="48.2432022" end_time="48.5912056" fwhm="0.116647489" area="1.50363679E+10" background="7.529986E+07" height="1.27919962E+09" mass_error_ppm="-0.2539824" truncated="0" identified="false" isotope_dotp="0.9896341" user_set="FALSE" />
          <precursor_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="51.97727" start_time="51.8188667" end_time="52.2604675" fwhm="0.1235175" area="5.49083E+10" background="5.411965E+08" height="4.35653939E+09" mass_error_ppm="-0.07503706" truncated="0" identified="false" isotope_dotp="0.9895853" user_set="FALSE" />
          <precursor_peak replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="51.5597" start_time="51.3567" end_time="51.7511024" fwhm="0.129469067" area="1.228389E+10" background="131782432" height="942803136" mass_error_ppm="-0.133473575" truncated="0" identified="false" isotope_dotp="0.987350643" user_set="FALSE" />
          <precursor_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="50.8114281" start_time="50.6224251" end_time="51.063427" fwhm="0.1252881" area="1.73988454E+10" background="212398112" height="1.28609434E+09" mass_error_ppm="-0.05501162" truncated="0" identified="false" isotope_dotp="0.983372867" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="59.5364761" start_time="59.3996773" end_time="59.7776756" fwhm="0.178802073" area="3.47481743E+10" background="307995648" height="2.1236855E+09" mass_error_ppm="0.65469265" truncated="0" identified="false" isotope_dotp="0.9862816" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="60.5891037" start_time="60.4595032" end_time="60.8663025" fwhm="0.195755661" area="3.99604122E+10" background="273861856" height="2.22030746E+09" mass_error_ppm="0.9896802" truncated="0" identified="false" isotope_dotp="0.971244633" user_set="FALSE" />
        </precursor_results>
        <transition fragment_type="precursor" isotope_dist_rank="1" isotope_dist_proportion="0.5991695">
          <precursor_mz>498.801809</precursor_mz>
          <product_mz>498.801809</product_mz>
          <collision_energy>18.730716</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="0.2" retention_time="48.5133171" start_time="48.3351173" end_time="48.701416" area="9.663761E+09" background="111150272" height="1.4982857E+09" fwhm="0.101717524" truncated="false" identified="false" rank="1" points_across="14" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="0" retention_time="48.4027023" start_time="48.2432022" end_time="48.5912056" area="9.217808E+09" background="3.822143E+07" height="1.27919962E+09" fwhm="0.110737741" truncated="false" identified="false" rank="1" points_across="11" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-0.1" retention_time="51.97727" start_time="51.8188667" end_time="52.2604675" area="3.48051579E+10" background="306692064" height="4.35653939E+09" fwhm="0.1235175" truncated="false" identified="false" rank="1" points_across="90" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="0.3" retention_time="51.5597" start_time="51.3567" end_time="51.7511024" area="7.445549E+09" background="68234824" height="942803136" fwhm="0.115074173" truncated="false" identified="false" rank="1" points_across="57" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="0.2" retention_time="50.8114281" start_time="50.6224251" end_time="51.063427" area="1.0548524E+10" background="1.410196E+08" height="1.28609434E+09" fwhm="0.123931989" truncated="false" identified="false" rank="1" points_across="18" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="1" retention_time="59.5364761" start_time="59.3996773" end_time="59.7776756" area="2.222889E+10" background="1.901218E+08" height="2.1236855E+09" fwhm="0.176827788" truncated="false" identified="false" rank="1" points_across="25" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="1.3" retention_time="60.5891037" start_time="60.4595032" end_time="60.8663025" area="2.64835748E+10" background="206070528" height="2.22030746E+09" fwhm="0.195755661" truncated="false" identified="false" rank="1" points_across="24" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="1" isotope_dist_rank="2" isotope_dist_proportion="0.272627026">
          <precursor_mz>498.801809</precursor_mz>
          <product_mz>499.303364</product_mz>
          <collision_energy>18.730716</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-0.5" retention_time="48.5133171" start_time="48.3351173" end_time="48.701416" area="4.66021274E+09" background="63243772" height="6.587257E+08" fwhm="0.114649728" truncated="false" identified="false" rank="2" points_across="14" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-0.7" retention_time="48.4027023" start_time="48.2432022" end_time="48.5912056" area="4.43400755E+09" background="37078424" height="6.289558E+08" fwhm="0.108036049" truncated="false" identified="false" rank="2" points_across="11" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="0.2" retention_time="51.9532661" start_time="51.8188667" end_time="52.2604675" area="1.51066214E+10" background="194241456" height="1.85227034E+09" fwhm="0.1217062" truncated="false" identified="false" rank="2" points_across="90" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.9" retention_time="51.5597" start_time="51.3567" end_time="51.7511024" area="3.68440678E+09" background="4.499914E+07" height="4.733107E+08" fwhm="0.114263915" truncated="false" identified="false" rank="2" points_across="57" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.6" retention_time="50.8114281" start_time="50.6224251" end_time="51.063427" area="5.287441E+09" background="62437616" height="6.467371E+08" fwhm="0.123430863" truncated="false" identified="false" rank="2" points_across="18" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0" retention_time="59.5688744" start_time="59.3996773" end_time="59.7776756" area="9.342552E+09" background="103299336" height="9.364951E+08" fwhm="0.178802073" truncated="false" identified="false" rank="2" points_across="25" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.5" retention_time="60.5675049" start_time="60.4595032" end_time="60.8663025" area="1.01285929E+10" background="62059104" height="8.903896E+08" fwhm="0.187280476" truncated="false" identified="false" rank="2" points_across="24" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="2" isotope_dist_rank="3" isotope_dist_proportion="0.07791711">
          <precursor_mz>498.801809</precursor_mz>
          <product_mz>499.804689</product_mz>
          <collision_energy>18.730716</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-0.2" retention_time="48.5133171" start_time="48.3351173" end_time="48.701416" area="1.16798541E+09" background="22846846" height="179703424" fwhm="0.104743332" truncated="false" identified="false" rank="3" points_across="14" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-0.5" retention_time="48.4027023" start_time="48.2432022" end_time="48.5912056" area="1.155634E+09" background="0" height="1.566255E+08" fwhm="0.113889135" truncated="false" identified="false" rank="3" points_across="11" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-0.7" retention_time="51.9532661" start_time="51.8188667" end_time="52.2604675" area="4.1795113E+09" background="29226908" height="536419168" fwhm="0.116321512" truncated="false" identified="false" rank="3" points_across="90" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.5" retention_time="51.5249023" start_time="51.3567" end_time="51.7511024" area="9.570047E+08" background="15011395" height="120146864" fwhm="0.129469067" truncated="false" identified="false" rank="3" points_across="57" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="0.1" retention_time="50.7844276" start_time="50.6224251" end_time="51.063427" area="1.30954534E+09" background="8940899" height="159020560" fwhm="0.1252881" truncated="false" identified="false" rank="3" points_across="18" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.1" retention_time="59.5148773" start_time="59.3996773" end_time="59.7776756" area="2.669706E+09" background="14574542" height="2.576329E+08" fwhm="0.169555992" truncated="false" identified="false" rank="3" points_across="25" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0" retention_time="60.6107025" start_time="60.4595032" end_time="60.8663025" area="2.797412E+09" background="5732235" height="245992944" fwhm="0.18911618" truncated="false" identified="false" rank="3" points_across="24" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="3" isotope_dist_rank="5" isotope_dist_proportion="0.01659727">
          <precursor_mz>498.801809</precursor_mz>
          <product_mz>500.305985</product_mz>
          <collision_energy>18.730716</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-0.3" retention_time="48.5133171" start_time="48.3351173" end_time="48.701416" area="227527648" background="0" height="35646076" fwhm="0.09206973" truncated="false" identified="false" rank="4" points_across="14" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-0.6" retention_time="48.4027023" start_time="48.2432022" end_time="48.5912056" area="228917088" background="0" height="29691456" fwhm="0.116647489" truncated="false" identified="false" rank="4" points_across="11" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-0.9" retention_time="51.9532661" start_time="51.8188667" end_time="52.2604675" area="8.170088E+08" background="11036043" height="104774512" fwhm="0.112673134" truncated="false" identified="false" rank="4" points_across="90" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.4" retention_time="51.5655022" start_time="51.3567" end_time="51.7511024" area="1.969281E+08" background="3537073.5" height="2.600372E+07" fwhm="0.123216458" truncated="false" identified="false" rank="4" points_across="57" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.1" retention_time="50.7844276" start_time="50.6224251" end_time="51.063427" area="253335168" background="0" height="3.013949E+07" fwhm="0.123529769" truncated="false" identified="false" rank="4" points_across="18" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.5" retention_time="59.5508766" start_time="59.3996773" end_time="59.7776756" area="507028128" background="0" height="57360684" fwhm="0.160780638" truncated="false" identified="false" rank="4" points_across="25" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.1" retention_time="60.5891037" start_time="60.4595032" end_time="60.8663025" area="5.508289E+08" background="0" height="55864708" fwhm="0.161665022" truncated="false" identified="false" rank="4" points_across="24" user_set="FALSE" />
          </transition_results>
        </transition>
      </precursor>
    </peptide>
    <peptide auto_manage_children="false" standard_type="iRT" sequence="NGFILDGFPR" modified_sequence="NGFILDGFPR" calc_neutral_pep_mass="1134.582194" num_missed_cleavages="0" rt_calculator_score="86.5529751205097" predicted_retention_time="57.25" avg_measured_retention_time="57.45777">
      <implicit_modifications>
        <implicit_heavy_modifications>
          <implicit_modification index_aa="9" modification_name="Label:13C(6)15N(4) (C-term R)" mass_diff="+10" />
        </implicit_heavy_modifications>
      </implicit_modifications>
      <peptide_results>
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="53.3049164" predicted_retention_time="53.4" />
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="53.2167053" predicted_retention_time="53.35" />
        <peptide_result replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="56.7436676" predicted_retention_time="56.56" />
        <peptide_result replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="55.9097023" predicted_retention_time="55.85" />
        <peptide_result replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="55.79743" predicted_retention_time="55.83" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="63.0788765" predicted_retention_time="62.45" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="64.15311" predicted_retention_time="63.3" />
      </peptide_results>
      <precursor charge="2" isotope_label="heavy" calc_neutral_mass="1144.590463" precursor_mz="573.302507" collision_energy="21.412741" modified_sequence="NGFILDGFPR[+10.0]">
        <precursor_results>
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="53.3049164" start_time="53.0871162" end_time="53.6217155" fwhm="0.1695711" area="3.2532222E+10" background="460546944" height="1.71790029E+09" mass_error_ppm="-0.0105123706" truncated="0" identified="false" isotope_dotp="0.993441641" user_set="FALSE" />
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="53.2167053" start_time="52.9702034" end_time="53.5067024" fwhm="0.164597392" area="3.54201641E+10" background="4.287057E+08" height="1.84745382E+09" mass_error_ppm="0.0345812477" truncated="0" identified="false" isotope_dotp="0.992980361" user_set="FALSE" />
          <precursor_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="56.7436676" start_time="56.5228653" end_time="57.0460663" fwhm="0.181724191" area="9.608682E+10" background="1.3007072E+09" height="4.57638E+09" mass_error_ppm="-0.0492766" truncated="0" identified="false" isotope_dotp="0.996502638" user_set="FALSE" />
          <precursor_peak replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="55.9097023" start_time="55.7009" end_time="56.1301" fwhm="0.144816935" area="2.45409341E+10" background="8.086222E+08" height="1.51004429E+09" mass_error_ppm="-0.6213692" truncated="0" identified="false" isotope_dotp="0.9847538" user_set="FALSE" />
          <precursor_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="55.79743" start_time="55.6174278" end_time="56.0854263" fwhm="0.156074166" area="4.05323039E+10" background="902014656" height="2.2611927E+09" mass_error_ppm="-0.288022161" truncated="0" identified="false" isotope_dotp="0.9944742" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="63.0788765" start_time="62.8736763" end_time="63.6044769" fwhm="0.30690366" area="1.06630767E+11" background="1.257255E+09" height="3.23971251E+09" mass_error_ppm="0.5050429" truncated="0" identified="false" isotope_dotp="0.9950197" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="64.15311" start_time="63.8795052" end_time="64.7543" fwhm="0.350876838" area="1.13878286E+11" background="871936960" height="3.06701261E+09" mass_error_ppm="0.5181425" truncated="0" identified="false" isotope_dotp="0.9880995" user_set="FALSE" />
        </precursor_results>
        <transition fragment_type="precursor" isotope_dist_rank="1" isotope_dist_proportion="0.540276945">
          <precursor_mz>573.302507</precursor_mz>
          <product_mz>573.302507</product_mz>
          <collision_energy>21.412741</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="0.6" retention_time="53.3049164" start_time="53.0871162" end_time="53.6217155" area="1.83988326E+10" background="226993360" height="1.71790029E+09" fwhm="0.1695711" truncated="false" identified="false" rank="1" points_across="25" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="0.6" retention_time="53.2167053" start_time="52.9702034" end_time="53.5067024" area="2.00762839E+10" background="197412656" height="1.84745382E+09" fwhm="0.162625626" truncated="false" identified="false" rank="1" points_across="24" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="0.3" retention_time="56.7436676" start_time="56.5228653" end_time="57.0460663" area="5.37254134E+10" background="6.688483E+08" height="4.57638E+09" fwhm="0.176940233" truncated="false" identified="false" rank="1" points_across="110" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.3" retention_time="55.9097023" start_time="55.7009" end_time="56.1301" area="1.40803072E+10" background="400097152" height="1.51004429E+09" fwhm="0.14255479" truncated="false" identified="false" rank="1" points_across="66" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="0" retention_time="55.79743" start_time="55.6174278" end_time="56.0854263" area="2.27178E+10" background="5.317432E+08" height="2.2611927E+09" fwhm="0.155151427" truncated="false" identified="false" rank="1" points_across="21" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.6" retention_time="63.0788765" start_time="62.8736763" end_time="63.6044769" area="6.03228242E+10" background="6.508115E+08" height="3.23971251E+09" fwhm="0.30690366" truncated="false" identified="false" rank="1" points_across="62" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.8" retention_time="64.15311" start_time="63.8795052" end_time="64.7543" area="6.509285E+10" background="5.683917E+08" height="3.06701261E+09" fwhm="0.350876838" truncated="false" identified="false" rank="1" points_across="80" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="1" isotope_dist_rank="2" isotope_dist_proportion="0.303479671">
          <precursor_mz>573.302507</precursor_mz>
          <product_mz>573.804029</product_mz>
          <collision_energy>21.412741</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-0.9" retention_time="53.3049164" start_time="53.0871162" end_time="53.6217155" area="1.01113354E+10" background="121507304" height="9.544261E+08" fwhm="0.167891428" truncated="false" identified="false" rank="2" points_across="25" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-0.8" retention_time="53.2167053" start_time="52.9702034" end_time="53.5067024" area="1.09925786E+10" background="151127856" height="9.957896E+08" fwhm="0.164597392" truncated="false" identified="false" rank="2" points_across="24" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-0.6" retention_time="56.7292671" start_time="56.5228653" end_time="57.0460663" area="3.0343764E+10" background="473405120" height="2.70175744E+09" fwhm="0.172742561" truncated="false" identified="false" rank="2" points_across="110" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-1" retention_time="55.9097023" start_time="55.7009" end_time="56.1301" area="7.6905385E+09" background="290242944" height="8.227958E+08" fwhm="0.14274165" truncated="false" identified="false" rank="2" points_across="66" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.8" retention_time="55.79743" start_time="55.6174278" end_time="56.0854263" area="1.2597634E+10" background="286073472" height="1.248301E+09" fwhm="0.156074166" truncated="false" identified="false" rank="2" points_across="21" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.3" retention_time="63.0752754" start_time="62.8736763" end_time="63.6044769" area="3.28488284E+10" background="4.308098E+08" height="1.82367718E+09" fwhm="0.2927842" truncated="false" identified="false" rank="2" points_across="62" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.2" retention_time="64.15311" start_time="63.8795052" end_time="64.7543" area="3.54085151E+10" background="234403792" height="1.7216599E+09" fwhm="0.3443006" truncated="false" identified="false" rank="2" points_across="80" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="2" isotope_dist_rank="3" isotope_dist_proportion="0.09957609">
          <precursor_mz>573.302507</precursor_mz>
          <product_mz>574.305391</product_mz>
          <collision_energy>21.412741</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-0.4" retention_time="53.3346176" start_time="53.0871162" end_time="53.6217155" area="3.181721E+09" background="88214216" height="301825920" fwhm="0.168785647" truncated="false" identified="false" rank="3" points_across="25" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-0.3" retention_time="53.2167053" start_time="52.9702034" end_time="53.5067024" area="3.4494976E+09" background="60393548" height="3.250735E+08" fwhm="0.160087481" truncated="false" identified="false" rank="3" points_across="24" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-0.2" retention_time="56.7292671" start_time="56.5228653" end_time="57.0460663" area="9.590941E+09" background="133360632" height="8.217126E+08" fwhm="0.17707625" truncated="false" identified="false" rank="3" points_across="110" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-1.5" retention_time="55.9329" start_time="55.7009" end_time="56.1301" area="2.25709824E+09" background="9.319244E+07" height="237451840" fwhm="0.144816935" truncated="false" identified="false" rank="3" points_across="66" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.2" retention_time="55.79743" start_time="55.6174278" end_time="56.0854263" area="4.11143E+09" background="7.676733E+07" height="4.139298E+08" fwhm="0.1548878" truncated="false" identified="false" rank="3" points_across="21" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.7" retention_time="63.093277" start_time="62.8736763" end_time="63.6044769" area="1.0764759E+10" background="136901264" height="6.39024E+08" fwhm="0.275682628" truncated="false" identified="false" rank="3" points_across="62" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="-0.1" retention_time="64.1423" start_time="63.8795052" end_time="64.7543" area="1.10031442E+10" background="69141496" height="5.437585E+08" fwhm="0.329686731" truncated="false" identified="false" rank="3" points_across="80" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="3" isotope_dist_rank="5" isotope_dist_proportion="0.0239409339">
          <precursor_mz>573.302507</precursor_mz>
          <product_mz>574.806706</product_mz>
          <collision_energy>21.412741</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-1.2" retention_time="53.3049164" start_time="53.0871162" end_time="53.6217155" area="840333440" background="23832072" height="8.243813E+07" fwhm="0.162821025" truncated="false" identified="false" rank="4" points_across="25" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-1.1" retention_time="53.2167053" start_time="52.9702034" end_time="53.5067024" area="901804544" background="19771658" height="8.258665E+07" fwhm="0.163174316" truncated="false" identified="false" rank="4" points_across="24" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-0.3" retention_time="56.7484665" start_time="56.5228653" end_time="57.0460663" area="2.42670054E+09" background="2.509311E+07" height="2.11735E+08" fwhm="0.181724191" truncated="false" identified="false" rank="4" points_across="110" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="0.1" retention_time="55.9097023" start_time="55.7009" end_time="56.1301" area="512988256" background="25089636" height="54954864" fwhm="0.144211054" truncated="false" identified="false" rank="4" points_across="66" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.7" retention_time="55.7884254" start_time="55.6174278" end_time="56.0854263" area="1.10544038E+09" background="7430598.5" height="110150344" fwhm="0.1546593" truncated="false" identified="false" rank="4" points_across="21" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.1" retention_time="63.0752754" start_time="62.8736763" end_time="63.6044769" area="2.69435366E+09" background="38732596" height="162609136" fwhm="0.261932433" truncated="false" identified="false" rank="4" points_across="62" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.4" retention_time="64.13151" start_time="63.8795052" end_time="64.7543" area="2.37378278E+09" background="0" height="119045312" fwhm="0.3418556" truncated="false" identified="false" rank="4" points_across="80" user_set="FALSE" />
          </transition_results>
        </transition>
      </precursor>
    </peptide>
    <peptide auto_manage_children="false" standard_type="iRT" sequence="LSSEAPALFQFDLK" modified_sequence="LSSEAPALFQFDLK" calc_neutral_pep_mass="1564.81371" num_missed_cleavages="0" rt_calculator_score="100" predicted_retention_time="63.08" avg_measured_retention_time="63.0587">
      <implicit_modifications>
        <implicit_heavy_modifications>
          <implicit_modification index_aa="13" modification_name="Label:13C(6)15N(2) (C-term K)" mass_diff="+8" />
        </implicit_heavy_modifications>
      </implicit_modifications>
      <peptide_results>
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="58.938015" predicted_retention_time="58.87" />
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="58.8427048" predicted_retention_time="58.84" />
        <peptide_result replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="62.77247" predicted_retention_time="62.37" />
        <peptide_result replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="61.1819" predicted_retention_time="61.35" />
        <peptide_result replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="62.025425" predicted_retention_time="61.53" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="68.561676" predicted_retention_time="68.81" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="69.08871" predicted_retention_time="69.77" />
      </peptide_results>
      <precursor charge="2" isotope_label="heavy" calc_neutral_mass="1572.827909" precursor_mz="787.42123" collision_energy="29.156909" modified_sequence="LSSEAPALFQFDLK[+8.0]">
        <precursor_results>
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="58.938015" start_time="58.7004166" end_time="59.2350159" fwhm="0.135334969" area="1.228578E+10" background="159162736" height="6.369843E+08" mass_error_ppm="0.211835846" truncated="0" identified="false" isotope_dotp="0.986492455" user_set="FALSE" />
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="58.8427048" start_time="58.6252022" end_time="59.0892029" fwhm="0.115631253" area="1.29981563E+10" background="106088480" height="8.382939E+08" mass_error_ppm="-0.288618624" truncated="0" identified="false" isotope_dotp="0.9807074" user_set="FALSE" />
          <precursor_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="62.77247" start_time="62.590065" end_time="62.969265" fwhm="0.110574752" area="3.93435873E+10" background="343837760" height="2.33715866E+09" mass_error_ppm="0.392305821" truncated="0" identified="false" isotope_dotp="0.9854064" user_set="FALSE" />
          <precursor_peak replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="61.1819" start_time="61.0079" end_time="61.3733025" fwhm="0.125015467" area="8.675413E+09" background="125859624" height="504133280" mass_error_ppm="-0.5933035" truncated="0" identified="false" isotope_dotp="0.9918391" user_set="FALSE" />
          <precursor_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="62.025425" start_time="61.818428" end_time="62.2054253" fwhm="0.107142806" area="9.261536E+09" background="371120128" height="5.58495E+08" mass_error_ppm="1.0032773" truncated="0" identified="false" isotope_dotp="0.982818365" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="68.561676" start_time="68.48968" end_time="68.67328" fwhm="0.07649118" area="1.563189E+10" background="111014184" height="1.36529677E+09" mass_error_ppm="0.661228955" truncated="0" identified="false" isotope_dotp="0.9805568" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="69.08871" start_time="69.031105" end_time="69.2111053" fwhm="0.0481538177" area="1.0191615E+10" background="160456064" height="1.51520832E+09" mass_error_ppm="0.521528959" truncated="0" identified="false" isotope_dotp="0.983816564" user_set="FALSE" />
        </precursor_results>
        <transition fragment_type="precursor" isotope_dist_rank="1" isotope_dist_proportion="0.423749655">
          <precursor_mz>787.42123</precursor_mz>
          <product_mz>787.42123</product_mz>
          <collision_energy>29.156909</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="1.5" retention_time="58.938015" start_time="58.7004166" end_time="59.2350159" area="5.51806874E+09" background="7.460741E+07" height="6.369843E+08" fwhm="0.127813935" truncated="false" identified="false" rank="1" points_across="27" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="0.3" retention_time="58.8427048" start_time="58.6252022" end_time="59.0892029" area="6.042474E+09" background="6.495075E+07" height="8.382939E+08" fwhm="0.109190375" truncated="false" identified="false" rank="1" points_across="19" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="1.2" retention_time="62.77247" start_time="62.590065" end_time="62.969265" area="1.70253865E+10" background="168560768" height="2.33715866E+09" fwhm="0.110574752" truncated="false" identified="false" rank="1" points_across="87" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-1.2" retention_time="61.1819" start_time="61.0079" end_time="61.3733025" area="3.86062669E+09" background="41030264" height="504133280" fwhm="0.121349454" truncated="false" identified="false" rank="1" points_across="65" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="1.1" retention_time="62.025425" start_time="61.818428" end_time="62.2054253" area="3.933539E+09" background="167290688" height="5.58495E+08" fwhm="0.107142806" truncated="false" identified="false" rank="1" points_across="18" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.7" retention_time="68.561676" start_time="68.48968" end_time="68.67328" area="6.58897766E+09" background="6.351768E+07" height="1.36529677E+09" fwhm="0.07649118" truncated="false" identified="false" rank="1" points_across="9" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.7" retention_time="69.08871" start_time="69.031105" end_time="69.2111053" area="4.706133E+09" background="85507784" height="1.51520832E+09" fwhm="0.0481538177" truncated="false" identified="false" rank="1" points_across="8" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="1" isotope_dist_rank="2" isotope_dist_proportion="0.338565379">
          <precursor_mz>787.42123</precursor_mz>
          <product_mz>787.92275</product_mz>
          <collision_energy>29.156909</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-0.9" retention_time="58.938015" start_time="58.7004166" end_time="59.2350159" area="4.108348E+09" background="57435624" height="4.920999E+08" fwhm="0.126784384" truncated="false" identified="false" rank="2" points_across="27" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-0.9" retention_time="58.8427048" start_time="58.6252022" end_time="59.0892029" area="4.25794458E+09" background="31214042" height="6.01399E+08" fwhm="0.1076855" truncated="false" identified="false" rank="2" points_across="19" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-0.4" retention_time="62.7868652" start_time="62.590065" end_time="62.969265" area="1.4539775E+10" background="136886880" height="2.05155315E+09" fwhm="0.109355316" truncated="false" identified="false" rank="2" points_across="87" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="0.5" retention_time="61.1819" start_time="61.0079" end_time="61.3733025" area="2.958179E+09" background="7.013795E+07" height="384815520" fwhm="0.122001067" truncated="false" identified="false" rank="2" points_across="65" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="1" retention_time="62.025425" start_time="61.818428" end_time="62.2054253" area="3.48194944E+09" background="144070992" height="495052960" fwhm="0.106026739" truncated="false" identified="false" rank="2" points_across="18" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.6" retention_time="68.561676" start_time="68.48968" end_time="68.67328" area="5.92178944E+09" background="2.842621E+07" height="1.2478007E+09" fwhm="0.07611841" truncated="false" identified="false" rank="2" points_across="9" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.1" retention_time="69.08871" start_time="69.031105" end_time="69.2111053" area="3.36518374E+09" background="34122556" height="1.08106918E+09" fwhm="0.0479674339" truncated="false" identified="false" rank="2" points_across="8" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="2" isotope_dist_rank="3" isotope_dist_proportion="0.1534849">
          <precursor_mz>787.42123</precursor_mz>
          <product_mz>788.424145</product_mz>
          <collision_energy>29.156909</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-0.9" retention_time="58.938015" start_time="58.7004166" end_time="59.2350159" area="2.06449958E+09" background="18630746" height="241148816" fwhm="0.1318983" truncated="false" identified="false" rank="3" points_across="27" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-0.8" retention_time="58.8427048" start_time="58.6252022" end_time="59.0892029" area="2.08904013E+09" background="9923689" height="2.978015E+08" fwhm="0.105536178" truncated="false" identified="false" rank="3" points_across="19" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="0.2" retention_time="62.762867" start_time="62.590065" end_time="62.969265" area="5.93962547E+09" background="38390104" height="8.508573E+08" fwhm="0.1080749" truncated="false" identified="false" rank="3" points_across="87" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-1.1" retention_time="61.1819" start_time="61.0079" end_time="61.3733025" area="1.368826E+09" background="3278718.5" height="175659520" fwhm="0.125015467" truncated="false" identified="false" rank="3" points_across="65" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="1.2" retention_time="62.025425" start_time="61.818428" end_time="62.2054253" area="1.38841613E+09" background="4.29902E+07" height="201594480" fwhm="0.104792029" truncated="false" identified="false" rank="3" points_across="18" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.8" retention_time="68.57967" start_time="68.48968" end_time="68.67328" area="2.306129E+09" background="0" height="481939648" fwhm="0.07616764" truncated="false" identified="false" rank="3" points_across="9" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.7" retention_time="69.08871" start_time="69.031105" end_time="69.2111053" area="1.61979366E+09" background="6912799.5" height="532040640" fwhm="0.04695815" truncated="false" identified="false" rank="3" points_across="8" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="3" isotope_dist_rank="4" isotope_dist_proportion="0.0504335053">
          <precursor_mz>787.42123</precursor_mz>
          <product_mz>788.925492</product_mz>
          <collision_energy>29.156909</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-0.2" retention_time="58.938015" start_time="58.7004166" end_time="59.2350159" area="594863552" background="8488963" height="7.030875E+07" fwhm="0.135334969" truncated="false" identified="false" rank="4" points_across="27" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-0.1" retention_time="58.8427048" start_time="58.6252022" end_time="59.0892029" area="6.086981E+08" background="0" height="81269336" fwhm="0.115631253" truncated="false" identified="false" rank="4" points_across="19" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-0.2" retention_time="62.7676659" start_time="62.590065" end_time="62.969265" area="1.83880064E+09" background="0" height="261124912" fwhm="0.109388143" truncated="false" identified="false" rank="4" points_across="87" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-1" retention_time="61.1819" start_time="61.0079" end_time="61.3733025" area="4.877814E+08" background="11412686" height="64257064" fwhm="0.117725939" truncated="false" identified="false" rank="4" points_across="65" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.4" retention_time="62.025425" start_time="61.818428" end_time="62.2054253" area="457631328" background="16768253" height="69043656" fwhm="0.09433436" truncated="false" identified="false" rank="4" points_across="18" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.4" retention_time="68.57967" start_time="68.48968" end_time="68.67328" area="8.149949E+08" background="19070298" height="174668688" fwhm="0.07468712" truncated="false" identified="false" rank="4" points_across="9" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="1.1" retention_time="69.08871" start_time="69.031105" end_time="69.2111053" area="500504832" background="3.391292E+07" height="181744112" fwhm="0.04269594" truncated="false" identified="false" rank="4" points_across="8" user_set="FALSE" />
          </transition_results>
        </transition>
      </precursor>
    </peptide>
    <peptide auto_manage_children="false" standard_type="iRT" sequence="ELASGLSFPVGFK" modified_sequence="ELASGLSFPVGFK" calc_neutral_pep_mass="1350.718353" num_missed_cleavages="0" rt_calculator_score="94.059335202468162" predicted_retention_time="60.5" avg_measured_retention_time="60.35027">
      <implicit_modifications>
        <implicit_heavy_modifications>
          <implicit_modification index_aa="12" modification_name="Label:13C(6)15N(2) (C-term K)" mass_diff="+8" />
        </implicit_heavy_modifications>
      </implicit_modifications>
      <peptide_results>
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="56.621418" predicted_retention_time="56.45" />
        <peptide_result replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="56.5517044" predicted_retention_time="56.41" />
        <peptide_result replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="60.1324654" predicted_retention_time="59.81" />
        <peptide_result replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="58.8503" predicted_retention_time="58.92" />
        <peptide_result replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="59.3524246" predicted_retention_time="59.01" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="64.97247" predicted_retention_time="66" />
        <peptide_result replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="65.97111" predicted_retention_time="66.91" />
      </peptide_results>
      <precursor charge="2" isotope_label="heavy" calc_neutral_mass="1358.732552" precursor_mz="680.373552" collision_energy="25.303192" modified_sequence="ELASGLSFPVGFK[+8.0]">
        <precursor_results>
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" peak_count_ratio="1" retention_time="56.621418" start_time="56.35412" end_time="56.8590164" fwhm="0.127528757" area="2.7942357E+10" background="136529824" height="1.62821171E+09" mass_error_ppm="0.452688873" truncated="0" identified="false" isotope_dotp="0.9887239" user_set="FALSE" />
          <precursor_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" peak_count_ratio="1" retention_time="56.5517044" start_time="56.3922043" end_time="56.8272057" fwhm="0.114715852" area="3.0845825E+10" background="8.130748E+08" height="2.12157478E+09" mass_error_ppm="0.531044066" truncated="0" identified="false" isotope_dotp="0.9807543" user_set="FALSE" />
          <precursor_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" peak_count_ratio="1" retention_time="60.1324654" start_time="59.95487" end_time="60.4444656" fwhm="0.1363981" area="8.107118E+10" background="1.12714368E+09" height="4.60321741E+09" mass_error_ppm="0.609922767" truncated="0" identified="false" isotope_dotp="0.991149" user_set="FALSE" />
          <precursor_peak replicate="20190130_RS_Hela_QC_1" peak_count_ratio="1" retention_time="58.8503" start_time="58.6473" end_time="59.0591" fwhm="0.126545325" area="1.81843784E+10" background="2.903324E+08" height="1.18285453E+09" mass_error_ppm="-0.36508584" truncated="0" identified="false" isotope_dotp="0.9840131" user_set="FALSE" />
          <precursor_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" peak_count_ratio="1" retention_time="59.3524246" start_time="59.17243" end_time="59.6314278" fwhm="0.138480663" area="2.700346E+10" background="1.01247238E+09" height="1.43895693E+09" mass_error_ppm="-0.227554038" truncated="0" identified="false" isotope_dotp="0.9933582" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" peak_count_ratio="1" retention_time="64.97247" start_time="64.6988754" end_time="65.40447" fwhm="0.234372258" area="6.78044959E+10" background="1.33550016E+09" height="2.40448026E+09" mass_error_ppm="0.526352346" truncated="0" identified="false" isotope_dotp="0.9824679" user_set="FALSE" />
          <precursor_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" peak_count_ratio="1" retention_time="65.97111" start_time="65.7299042" end_time="66.37791" fwhm="0.23208642" area="7.54204344E+10" background="1.20760358E+09" height="2.47791718E+09" mass_error_ppm="0.220725864" truncated="0" identified="false" isotope_dotp="0.989687145" user_set="FALSE" />
        </precursor_results>
        <transition fragment_type="precursor" isotope_dist_rank="1" isotope_dist_proportion="0.474336535">
          <precursor_mz>680.373552</precursor_mz>
          <product_mz>680.373552</product_mz>
          <collision_energy>25.303192</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="1.8" retention_time="56.621418" start_time="56.35412" end_time="56.8590164" area="1.41218929E+10" background="1.474533E+07" height="1.62821171E+09" fwhm="0.125305325" truncated="false" identified="false" rank="1" points_across="21" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="1.7" retention_time="56.5517044" start_time="56.3922043" end_time="56.8272057" area="1.59394765E+10" background="321127168" height="2.12157478E+09" fwhm="0.109827168" truncated="false" identified="false" rank="1" points_across="19" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="1.3" retention_time="60.1324654" start_time="59.95487" end_time="60.4444656" area="4.07443E+10" background="5.485225E+08" height="4.60321741E+09" fwhm="0.128599912" truncated="false" identified="false" rank="1" points_across="104" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.2" retention_time="58.8503" start_time="58.6473" end_time="59.0591" area="9.286599E+09" background="1.419469E+08" height="1.18285453E+09" fwhm="0.120431535" truncated="false" identified="false" rank="1" points_across="64" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="0.3" retention_time="59.3524246" start_time="59.17243" end_time="59.6314278" area="1.33995387E+10" background="369259968" height="1.43895693E+09" fwhm="0.1367099" truncated="false" identified="false" rank="1" points_across="23" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.4" retention_time="64.97247" start_time="64.6988754" end_time="65.40447" area="3.49962732E+10" background="747379648" height="2.40448026E+09" fwhm="0.231309235" truncated="false" identified="false" rank="1" points_across="73" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.2" retention_time="65.97111" start_time="65.7299042" end_time="66.37791" area="3.58735462E+10" background="138121968" height="2.47791718E+09" fwhm="0.23208642" truncated="false" identified="false" rank="1" points_across="68" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="1" isotope_dist_rank="2" isotope_dist_proportion="0.32841897">
          <precursor_mz>680.373552</precursor_mz>
          <product_mz>680.875072</product_mz>
          <collision_energy>25.303192</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-0.7" retention_time="56.621418" start_time="56.35412" end_time="56.8590164" area="9.255464E+09" background="64944548" height="1.07499648E+09" fwhm="0.124730125" truncated="false" identified="false" rank="2" points_across="21" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-0.5" retention_time="56.5662041" start_time="56.3922043" end_time="56.8272057" area="1.00943616E+10" background="286540544" height="1.349785E+09" fwhm="0.112974189" truncated="false" identified="false" rank="2" points_across="19" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="0.1" retention_time="60.1228676" start_time="59.95487" end_time="60.4444656" area="2.67436954E+10" background="404250944" height="2.89269248E+09" fwhm="0.133366" truncated="false" identified="false" rank="2" points_across="104" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.8" retention_time="58.8155022" start_time="58.6473" end_time="59.0591" area="6.03208E+09" background="109687176" height="7.687345E+08" fwhm="0.124216534" truncated="false" identified="false" rank="2" points_across="64" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.9" retention_time="59.3434258" start_time="59.17243" end_time="59.6314278" area="8.966766E+09" background="354545664" height="9.708674E+08" fwhm="0.138480663" truncated="false" identified="false" rank="2" points_across="23" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.8" retention_time="64.96887" start_time="64.6988754" end_time="65.40447" area="2.17830359E+10" background="351668160" height="1.46932262E+09" fwhm="0.233268023" truncated="false" identified="false" rank="2" points_across="73" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.1" retention_time="65.9639053" start_time="65.7299042" end_time="66.37791" area="2.57522811E+10" background="6.376973E+08" height="1.81241331E+09" fwhm="0.227750361" truncated="false" identified="false" rank="2" points_across="68" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="2" isotope_dist_rank="3" isotope_dist_proportion="0.130098522">
          <precursor_mz>680.373552</precursor_mz>
          <product_mz>681.37646</product_mz>
          <collision_energy>25.303192</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-1.4" retention_time="56.621418" start_time="56.35412" end_time="56.8590164" area="3.56886374E+09" background="4.225246E+07" height="4.162605E+08" fwhm="0.126083523" truncated="false" identified="false" rank="3" points_across="21" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-1.2" retention_time="56.5662041" start_time="56.3922043" end_time="56.8272057" area="3.76255258E+09" background="146912768" height="507075232" fwhm="0.111037835" truncated="false" identified="false" rank="3" points_across="19" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-0.5" retention_time="60.1228676" start_time="59.95487" end_time="60.4444656" area="1.05992663E+10" background="134393712" height="1.1501079E+09" fwhm="0.1363981" truncated="false" identified="false" rank="3" points_across="104" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="0.2" retention_time="58.8503" start_time="58.6473" end_time="59.0591" area="2.20443059E+09" background="23114378" height="279653920" fwhm="0.121332459" truncated="false" identified="false" rank="3" points_across="64" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-0.2" retention_time="59.3524246" start_time="59.17243" end_time="59.6314278" area="3.66458266E+09" background="214700912" height="4.198807E+08" fwhm="0.13551034" truncated="false" identified="false" rank="3" points_across="23" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.3" retention_time="64.94008" start_time="64.6988754" end_time="65.40447" area="8.633779E+09" background="1.845175E+08" height="586797632" fwhm="0.234372258" truncated="false" identified="false" rank="3" points_across="73" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.5" retention_time="65.99631" start_time="65.7299042" end_time="66.37791" area="1.08283965E+10" background="295098560" height="7.629543E+08" fwhm="0.231734753" truncated="false" identified="false" rank="3" points_across="68" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="precursor" mass_index="3" isotope_dist_rank="4" isotope_dist_proportion="0.0374834836">
          <precursor_mz>680.373552</precursor_mz>
          <product_mz>681.877798</product_mz>
          <collision_energy>25.303192</collision_energy>
          <transition_results>
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_1" mass_error_ppm="-1.3" retention_time="56.621418" start_time="56.35412" end_time="56.8590164" area="996137152" background="14587493" height="110085840" fwhm="0.127528757" truncated="false" identified="false" rank="4" points_across="21" user_set="FALSE" />
            <transition_peak replicate="20190129_FPL_M1_Hela_1ug_PPRT_500fm_QC_2" mass_error_ppm="-1.1" retention_time="56.5662041" start_time="56.3922043" end_time="56.8272057" area="1.04943322E+09" background="5.849431E+07" height="1.390479E+08" fwhm="0.114715852" truncated="false" identified="false" rank="4" points_across="19" user_set="FALSE" />
            <transition_peak replicate="20191029_RS_Hela_QC_1ug_Tr_High_11ms_1" mass_error_ppm="-0.3" retention_time="60.1372681" start_time="59.95487" end_time="60.4444656" area="2.98391014E+09" background="3.997652E+07" height="3.257227E+08" fwhm="0.130296409" truncated="false" identified="false" rank="4" points_across="104" user_set="FALSE" />
            <transition_peak replicate="20190130_RS_Hela_QC_1" mass_error_ppm="-0.6" retention_time="58.8097" start_time="58.6473" end_time="59.0591" area="6.61268E+08" background="15583957" height="8.392456E+07" fwhm="0.126545325" truncated="false" identified="false" rank="4" points_across="64" user_set="FALSE" />
            <transition_peak replicate="20190725_FPL_Hela_1ug_PPRT_500fm_QC_1 (Fusion ES803A)" mass_error_ppm="-1.4" retention_time="59.3434258" start_time="59.17243" end_time="59.6314278" area="972573632" background="7.396582E+07" height="105935568" fwhm="0.135593832" truncated="false" identified="false" rank="4" points_across="23" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol (SP25 column)" mass_error_ppm="0.7" retention_time="64.94008" start_time="64.6988754" end_time="65.40447" area="2.39140659E+09" background="51934904" height="172755024" fwhm="0.220319629" truncated="false" identified="false" rank="4" points_across="73" user_set="FALSE" />
            <transition_peak replicate="20190731_HeLa_1ug_PPRT_500fmol_20190801190736 (SP25 new)" mass_error_ppm="0.5" retention_time="65.97111" start_time="65.7299042" end_time="66.37791" area="2.96621184E+09" background="136685744" height="210349312" fwhm="0.229607165" truncated="false" identified="false" rank="4" points_across="68" user_set="FALSE" />
          </transition_results>
        </transition>
      </precursor>
    </peptide>
  </peptide_list>
</srm_settings>