﻿<?xml version="1.0" encoding="utf-8"?>
<srm_settings format_version="19.1" software_version="Skyline (64-bit) 19.1.0.193">
  <settings_summary name="Default">
    <peptide_settings>
      <enzyme name="Trypsin" cut="KR" no_cut="P" sense="C" />
      <digest_settings max_missed_cleavages="2" />
      <peptide_prediction use_measured_rts="false" measured_rt_window="5" use_spectral_library_drift_times="false" spectral_library_drift_times_peak_width_calc_type="resolving_power" spectral_library_drift_times_resolving_power="0" spectral_library_drift_times_width_at_dt_zero="0" spectral_library_drift_times_width_at_dt_max="0">
        <predict_retention_time name="iRT" time_window="10">
          <irt_calculator name="iRT" database_path="D:\MS_DATA_HFX\iRT2to30.irtdb" />
        </predict_retention_time>
      </peptide_prediction>
      <peptide_filter start="25" min_length="8" max_length="25" auto_select="true">
        <peptide_exclusions />
      </peptide_filter>
      <peptide_libraries pick="library" />
      <peptide_modifications max_variable_mods="3" max_neutral_losses="1" internal_standard="none">
        <static_modifications>
          <static_modification name="Carbamidomethyl (C)" aminoacid="C" formula="H3C2NO" unimod_id="4" short_name="CAM" />
          <static_modification name="Deamidated (NQ)" aminoacid="N, Q" variable="true" formula="O - HN" unimod_id="7" short_name="Dea" />
          <static_modification name="Oxidation (M)" aminoacid="M" variable="true" formula="O" unimod_id="35" short_name="Oxi">
            <potential_loss formula="H4COS" massdiff_monoisotopic="63.998285" massdiff_average="64.10701" />
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        </static_modifications>
        <heavy_modifications />
      </peptide_modifications>
      <quantification weighting="none" fit="none" normalization="none" ms_level="2" units="pmol" />
    </peptide_settings>
    <transition_settings>
      <transition_prediction precursor_mass_type="Monoisotopic" fragment_mass_type="Monoisotopic" optimize_by="None">
        <predict_collision_energy name="Thermo TSQ Ultra" step_size="1" step_count="5">
          <regression_ce charge="2" slope="0.036" intercept="0.954" />
          <regression_ce charge="3" slope="0.037" intercept="3.525" />
        </predict_collision_energy>
      </transition_prediction>
      <transition_filter precursor_charges="2" product_charges="1" precursor_adducts="[M-3H],[M-2H],[M-H],[M-],[M+H],[M+],[M+2H],[M+3H]" product_adducts="[M-3],[M-2],[M-],[M+],[M+2],[M+3]" fragment_types="p,b,y" small_molecule_fragment_types="f,p" fragment_range_first="(m/z &gt; precursor) - 1" fragment_range_last="last ion - 3" precursor_mz_window="0" auto_select="true">
        <measured_ion name="TMT-131" ion_formula="C4C'4H16N'" mass_monoisotopic="131.1387288096" mass_average="131.1839682496" charge="1" optional="true" />
      </transition_filter>
      <transition_libraries ion_match_tolerance="0.05" min_ion_count="0" ion_count="6" pick_from="none" />
      <transition_integration integrate_all="true" />
      <transition_instrument dynamic_min="true" min_mz="350" max_mz="1550" mz_match_tolerance="0.02" />
      <transition_full_scan acquisition_method="DDA" product_mass_analyzer="orbitrap" product_res="60000" product_res_mz="400" precursor_isotopes="Count" precursor_isotope_filter="4" precursor_mass_analyzer="orbitrap" precursor_res="60000" precursor_res_mz="400" selective_extraction="true">
        <isotope_enrichments name="Default">
          <atom_percent_enrichment symbol="H'">0.98</atom_percent_enrichment>
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          <atom_percent_enrichment symbol="N'">0.995</atom_percent_enrichment>
          <atom_percent_enrichment symbol="O&quot;">0.99</atom_percent_enrichment>
          <atom_percent_enrichment symbol="O'">0.99</atom_percent_enrichment>
        </isotope_enrichments>
      </transition_full_scan>
    </transition_settings>
    <data_settings document_guid="3ddc11d3-2026-4f04-b818-8e0525985f71">
      <annotation name="Hela digest (ug)" targets="replicate" type="value_list">
        <value>1</value>
        <value>2</value>
      </annotation>
      <annotation name="PPRT (fmol)" targets="replicate" type="value_list">
        <value>250</value>
        <value>500</value>
        <value>1000</value>
      </annotation>
    </data_settings>
    <measured_results time_normal_area="true">
      <replicate name="20210721_EvoHFX_HeLaQC_500ng_1">
        <sample_file id="_20210721_EvoHFX_HeLaQC_500ng_1_f0" file_path="D:\MS_DATA_HFX\20210721_EvoHFX_HeLaQC_500ng_1.raw" sample_name="20210721_EvoHFX_HeLaQC_500ng_1" acquired_time="2021-07-21T19:23:43" modified_time="2021-07-21T20:07:45" tic_area="386646867968" ion_mobility_type="compensation_V">
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            <instrument_info>
              <model>Q Exactive</model>
              <ionsource>nanoelectrospray</ionsource>
              <analyzer>quadrupole/orbitrap</analyzer>
              <detector>inductive detector</detector>
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        </sample_file>
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      <replicate name="20210721_EvoHFX_HeLaQC_500ng_2">
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          <instrument_info_list>
            <instrument_info>
              <model>Q Exactive</model>
              <ionsource>nanoelectrospray</ionsource>
              <analyzer>quadrupole/orbitrap</analyzer>
              <detector>inductive detector</detector>
            </instrument_info>
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        </sample_file>
      </replicate>
      <replicate name="20210511_EvoCol_HEK_500ng_1">
        <sample_file id="_20210511_EvoCol_HEK_500ng_1_f0" file_path="Z:\Collaboration_Services\Raw_files_FPL\Fusion\2021\EvoSep_run_in\20210511_EvoCol_HEK_500ng_1.raw" sample_name="20210511_EvoCol_HEK_500ng_1" acquired_time="2021-05-12T15:00:25" modified_time="2021-05-12T15:44:26" tic_area="32055351296" ion_mobility_type="compensation_V">
          <instrument_info_list>
            <instrument_info>
              <model>Orbitrap Fusion</model>
              <ionsource>nanoelectrospray</ionsource>
              <analyzer>quadrupole/orbitrap</analyzer>
              <detector>inductive detector</detector>
            </instrument_info>
            <instrument_info>
              <model>Orbitrap Fusion</model>
              <ionsource>nanoelectrospray</ionsource>
              <analyzer>quadrupole/radial ejection linear ion trap</analyzer>
              <detector>electron multiplier</detector>
            </instrument_info>
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      <replicate name="20210511_EvoCol_HEK_500ng_2">
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          <instrument_info_list>
            <instrument_info>
              <model>Orbitrap Fusion</model>
              <ionsource>nanoelectrospray</ionsource>
              <analyzer>quadrupole/orbitrap</analyzer>
              <detector>inductive detector</detector>
            </instrument_info>
            <instrument_info>
              <model>Orbitrap Fusion</model>
              <ionsource>nanoelectrospray</ionsource>
              <analyzer>quadrupole/radial ejection linear ion trap</analyzer>
              <detector>electron multiplier</detector>
            </instrument_info>
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        </sample_file>
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      <replicate name="20210511_EvoCol_HEK_1ug_1">
        <sample_file id="_20210511_EvoCol_HEK_1ug_1_f0" file_path="Z:\Collaboration_Services\Raw_files_FPL\Fusion\2021\EvoSep_run_in\20210511_EvoCol_HEK_1ug_1.raw" sample_name="20210511_EvoCol_HEK_1ug_1" acquired_time="2021-05-12T16:37:14" modified_time="2021-05-12T17:21:15" tic_area="97496678400" ion_mobility_type="compensation_V">
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            <instrument_info>
              <model>Orbitrap Fusion</model>
              <ionsource>nanoelectrospray</ionsource>
              <analyzer>quadrupole/orbitrap</analyzer>
              <detector>inductive detector</detector>
            </instrument_info>
            <instrument_info>
              <model>Orbitrap Fusion</model>
              <ionsource>nanoelectrospray</ionsource>
              <analyzer>quadrupole/radial ejection linear ion trap</analyzer>
              <detector>electron multiplier</detector>
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      <replicate name="20210511_EvoCol_HEK_1ug_2">
        <sample_file id="_20210511_EvoCol_HEK_1ug_2_f0" file_path="Z:\Collaboration_Services\Raw_files_FPL\Fusion\2021\EvoSep_run_in\20210511_EvoCol_HEK_1ug_2.raw" sample_name="20210511_EvoCol_HEK_1ug_2" acquired_time="2021-05-12T17:25:40" modified_time="2021-05-12T18:09:41" tic_area="60025339904" ion_mobility_type="compensation_V">
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            <instrument_info>
              <model>Orbitrap Fusion</model>
              <ionsource>nanoelectrospray</ionsource>
              <analyzer>quadrupole/orbitrap</analyzer>
              <detector>inductive detector</detector>
            </instrument_info>
            <instrument_info>
              <model>Orbitrap Fusion</model>
              <ionsource>nanoelectrospray</ionsource>
              <analyzer>quadrupole/radial ejection linear ion trap</analyzer>
              <detector>electron multiplier</detector>
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      <replicate name="20211028_Dynamo_HeLaQC_500ng_2_EvoCol">
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              <model>Q Exactive</model>
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      <replicate name="20211101_HeLaQC_500ng_EvoCol_1">
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              <model>Q Exactive</model>
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      <replicate name="20211101_HeLaQC_500ng_EvoCol_2">
        <sample_file id="_20211101_HeLaQC_500ng_EvoCol_2_f0" file_path="D:\MS_DATA_HFX\20211101_HeLaQC_500ng_EvoCol_2.raw" sample_name="20211101_HeLaQC_500ng_EvoCol_2" acquired_time="2021-11-01T13:25:16" modified_time="2021-11-01T14:09:16" tic_area="485304434688" ion_mobility_type="compensation_V">
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              <model>Q Exactive</model>
              <ionsource>nanoelectrospray</ionsource>
              <analyzer>quadrupole/orbitrap</analyzer>
              <detector>inductive detector</detector>
            </instrument_info>
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      <replicate name="20211101_HeLaQC_500ng_EvoCol_3">
        <sample_file id="_20211101_HeLaQC_500ng_EvoCol_3_f0" file_path="D:\MS_DATA_HFX\20211101_HeLaQC_500ng_EvoCol_3.raw" sample_name="20211101_HeLaQC_500ng_EvoCol_3" acquired_time="2021-11-01T14:13:40" modified_time="2021-11-01T14:57:42" tic_area="477072424960" ion_mobility_type="compensation_V">
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            <instrument_info>
              <model>Q Exactive</model>
              <ionsource>nanoelectrospray</ionsource>
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              <detector>inductive detector</detector>
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      <replicate name="20211101_HeLaQC_500ng_EvoCol_4">
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            <instrument_info>
              <model>Q Exactive</model>
              <ionsource>nanoelectrospray</ionsource>
              <analyzer>quadrupole/orbitrap</analyzer>
              <detector>inductive detector</detector>
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      <replicate name="20211101_HeLaQC_500ng_EvoCol_5">
        <sample_file id="_20211101_HeLaQC_500ng_EvoCol_5_f0" file_path="D:\MS_DATA_HFX\20211101_HeLaQC_500ng_EvoCol_5.raw" sample_name="20211101_HeLaQC_500ng_EvoCol_5" acquired_time="2021-11-01T15:50:38" modified_time="2021-11-01T16:34:41" tic_area="377932152832" ion_mobility_type="compensation_V">
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            <instrument_info>
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              <ionsource>nanoelectrospray</ionsource>
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              <detector>inductive detector</detector>
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      <replicate name="20211101_HeLaQC_500ng_EvoCol_6_newIPA">
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      <replicate name="20211101_HeLaQC_500ng_EvoCol_7_newIPA">
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      <replicate name="20211101_HeLaQC_500ng_EvoCol_8_newIPA">
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              <model>Q Exactive</model>
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      <replicate name="20211101_HeLaQC_500ng_EvoCol_9_newIPA">
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      <replicate name="20211101_HeLaQC_500ng_EvoCol_10_newIPA">
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      <replicate name="20211101_HeLaQC_500ng_EvoCol_12">
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      <replicate name="20211101_HeLaQC_500ng_EvoCol_13">
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              <model>Q Exactive</model>
              <ionsource>nanoelectrospray</ionsource>
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              <detector>inductive detector</detector>
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      <replicate name="20211101_HeLaQC_500ng_EvoCol_14">
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              <model>Q Exactive</model>
              <ionsource>nanoelectrospray</ionsource>
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              <detector>inductive detector</detector>
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      <replicate name="20211101_HeLaQC_500ng_EvoCol_15">
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              <ionsource>nanoelectrospray</ionsource>
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              <detector>inductive detector</detector>
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      <replicate name="20211101_HeLaQC_500ng_EvoCol_16_newIPA">
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              <model>Q Exactive</model>
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            <transition_peak replicate="20210511_EvoCol_HEK_1ug_2" retention_time="10.2664938" start_time="10.2664938" end_time="10.5310936" area="0" background="0" height="0" fwhm="0.2645855" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="1" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211028_Dynamo_HeLaQC_500ng_2_EvoCol" retention_time="9.30927849" start_time="9.30927849" end_time="9.649579" area="0" background="0" height="0" fwhm="0.3402872" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="1" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_1" retention_time="7.24848" start_time="7.24848" end_time="8.57838" area="0" background="0" height="0" fwhm="1.32991743" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="5" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_2" retention_time="10.554285" start_time="10.554285" end_time="10.9382849" area="0" background="0" height="0" fwhm="0.384063721" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_3" retention_time="10.2862816" start_time="10.2862816" end_time="10.8382816" area="0" background="0" height="0" fwhm="0.55196" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="3" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_4" retention_time="10.9902849" start_time="10.9902849" end_time="11.3862848" area="0" background="0" height="0" fwhm="0.3959713" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="1" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_5" retention_time="11.0182819" start_time="11.0182819" end_time="11.5782824" area="0" background="0" height="0" fwhm="0.5599594" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_6_newIPA" retention_time="11.1342821" start_time="11.1342821" end_time="11.622282" area="0" background="0" height="0" fwhm="0.48796463" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_7_newIPA" retention_time="10.7502794" start_time="10.7502794" end_time="11.1742792" area="0" background="0" height="0" fwhm="0.423969269" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="1" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_8_newIPA" retention_time="10.3942776" start_time="10.3942776" end_time="10.7502775" area="0" background="0" height="0" fwhm="0.3559742" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="1" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_9_newIPA" retention_time="10.3302813" start_time="10.3302813" end_time="10.6822815" area="0" background="0" height="0" fwhm="0.3519745" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="1" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_10_newIPA" retention_time="10.5582762" start_time="10.5582762" end_time="10.8382769" area="0" background="0" height="0" fwhm="0.280046463" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="1" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_12" retention_time="9.102311" start_time="9.102311" end_time="9.638311" area="0" background="0" height="0" fwhm="0.536088943" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_13" retention_time="10.2662878" start_time="10.2662878" end_time="10.8022881" area="0" background="0" height="0" fwhm="0.536088943" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_14" retention_time="11.02308" start_time="11.02308" end_time="11.40438" area="0" background="0" height="0" fwhm="0.381285667" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_15" retention_time="11.4902792" start_time="11.4902792" end_time="11.9582787" area="0" background="0" height="0" fwhm="0.46796608" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_16_newIPA" retention_time="11.3342829" start_time="11.3342829" end_time="11.7942829" area="0" background="0" height="0" fwhm="0.45996666" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
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    </peptide>
    <peptide standard_type="iRT" sequence="GAGSSEPVTGLDAK" modified_sequence="GAGSSEPVTGLDAK" calc_neutral_pep_mass="1287.63066" num_missed_cleavages="0" rt_calculator_score="23.799650158556986" predicted_retention_time="16.98" avg_measured_retention_time="16.2654743">
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        <peptide_result replicate="20210511_EvoCol_HEK_1ug_2" peak_count_ratio="0.4" retention_time="16.1146946" predicted_retention_time="16.87" />
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        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_1" peak_count_ratio="0.6" retention_time="15.57498" predicted_retention_time="15.74" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_2" peak_count_ratio="0.7" retention_time="16.6782856" predicted_retention_time="17.48" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_3" peak_count_ratio="0.7" retention_time="17.2142811" predicted_retention_time="17.83" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_4" peak_count_ratio="0.7" retention_time="17.1982841" predicted_retention_time="18.1" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_5" peak_count_ratio="0.8" retention_time="17.2982826" predicted_retention_time="18.15" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_6_newIPA" peak_count_ratio="0.7" retention_time="17.2382813" predicted_retention_time="18.14" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_7_newIPA" peak_count_ratio="0.5" retention_time="16.70628" predicted_retention_time="17.42" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_8_newIPA" peak_count_ratio="0.5" retention_time="16.1382771" predicted_retention_time="16.82" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_9_newIPA" peak_count_ratio="0.5" retention_time="15.8542814" predicted_retention_time="16.46" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_10_newIPA" peak_count_ratio="0.7" retention_time="15.3782768" predicted_retention_time="16.32" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_12" peak_count_ratio="0.6" retention_time="15.038311" predicted_retention_time="15.63" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_13" peak_count_ratio="0.8" retention_time="15.9222879" predicted_retention_time="16.94" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_14" peak_count_ratio="0.8" retention_time="16.83688" predicted_retention_time="17.78" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_15" peak_count_ratio="0.8" retention_time="17.49828" predicted_retention_time="18.49" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_16_newIPA" peak_count_ratio="0.7" retention_time="17.6262836" predicted_retention_time="18.57" />
      </peptide_results>
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        <precursor_results>
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          <precursor_peak replicate="20210511_EvoCol_HEK_1ug_1" peak_count_ratio="0.4" retention_time="16.5304928" start_time="16.2766933" end_time="17.032692" fwhm="0.07403105" area="1.19437248E+09" background="0" height="127836752" mass_error_ppm="-0.6303418" truncated="0" identified="false" isotope_dotp="0.989476562" user_set="TRUE" />
          <precursor_peak replicate="20210511_EvoCol_HEK_1ug_2" peak_count_ratio="0.4" retention_time="16.1146946" start_time="15.8716936" end_time="16.4332943" fwhm="0.143179163" area="5.91663E+08" background="6699628" height="29438714" mass_error_ppm="-0.855628967" truncated="0" identified="false" isotope_dotp="0.982825637" user_set="FALSE" />
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          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_3" peak_count_ratio="0.7" retention_time="17.2142811" start_time="16.95428" end_time="17.50628" fwhm="0.327151775" area="4.594138E+09" background="78896984" height="186112224" mass_error_ppm="0.78333056" truncated="0" identified="false" isotope_dotp="0.989900351" user_set="FALSE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_4" peak_count_ratio="0.7" retention_time="17.1982841" start_time="16.9222851" end_time="17.5582848" fwhm="0.268255949" area="5.106085E+09" background="42750144" height="183821968" mass_error_ppm="0.764257" truncated="0" identified="false" isotope_dotp="0.9964704" user_set="FALSE" />
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          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_9_newIPA" peak_count_ratio="0.5" retention_time="15.8542814" start_time="15.5422812" end_time="16.1582813" fwhm="0.199594915" area="4.768E+09" background="36094476" height="190259376" mass_error_ppm="0.8808485" truncated="0" identified="false" isotope_dotp="0.9785896" user_set="FALSE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_10_newIPA" peak_count_ratio="0.7" retention_time="15.3782768" start_time="15.0702763" end_time="15.626276" fwhm="0.165188223" area="3.50618522E+09" background="69077176" height="171038720" mass_error_ppm="0.587667167" truncated="0" identified="false" isotope_dotp="0.9920867" user_set="TRUE" />
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          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_13" peak_count_ratio="0.8" retention_time="15.9222879" start_time="15.6782885" end_time="16.2022877" fwhm="0.267152518" area="3.0075648E+09" background="8.136893E+07" height="122004488" mass_error_ppm="0.904762864" truncated="0" identified="false" isotope_dotp="0.965394" user_set="TRUE" />
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          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_15" peak_count_ratio="0.8" retention_time="17.49828" start_time="17.26628" end_time="17.9902782" fwhm="0.270105422" area="2.93311027E+09" background="34732028" height="106246336" mass_error_ppm="0.859474957" truncated="0" identified="false" isotope_dotp="0.9919709" user_set="TRUE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_16_newIPA" peak_count_ratio="0.7" retention_time="17.6262836" start_time="17.3382835" end_time="17.8622837" fwhm="0.214849666" area="4.44676E+09" background="11493097" height="176990128" mass_error_ppm="0.844881058" truncated="0" identified="false" isotope_dotp="0.9914498" user_set="TRUE" />
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          <product_mz>644.822606</product_mz>
          <collision_energy>24.167614</collision_energy>
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            <transition_peak replicate="20210721_EvoHFX_HeLaQC_500ng_2" mass_error_ppm="0.8" retention_time="14.5980473" start_time="14.4422474" end_time="14.757947" area="3.43398042E+09" background="0" height="6.024566E+08" fwhm="0.08720881" truncated="false" identified="false" rank="1" points_across="21" user_set="FALSE" />
            <transition_peak replicate="20210511_EvoCol_HEK_500ng_1" mass_error_ppm="-1.3" retention_time="16.5088921" start_time="16.319891" end_time="16.8328915" area="2.32501325E+09" background="3.891859E+07" height="2.730202E+08" fwhm="0.1178688" truncated="false" identified="false" rank="1" points_across="48" user_set="FALSE" />
            <transition_peak replicate="20210511_EvoCol_HEK_500ng_2" mass_error_ppm="-0.4" retention_time="16.4872875" start_time="16.1686878" end_time="16.7896881" area="1.00341114E+09" background="800053.4" height="93519656" fwhm="0.144577861" truncated="false" identified="false" rank="1" points_across="71" user_set="TRUE" />
            <transition_peak replicate="20210511_EvoCol_HEK_1ug_1" mass_error_ppm="-0.6" retention_time="16.5304928" start_time="16.2766933" end_time="17.032692" area="6.040326E+08" background="0" height="127836752" fwhm="0.07403105" truncated="false" identified="false" rank="1" points_across="77" user_set="TRUE" />
            <transition_peak replicate="20210511_EvoCol_HEK_1ug_2" mass_error_ppm="-0.7" retention_time="16.1146946" start_time="15.8716936" end_time="16.4332943" area="282075072" background="4041893" height="29438714" fwhm="0.143179163" truncated="false" identified="false" rank="1" points_across="75" user_set="FALSE" />
            <transition_peak replicate="20211028_Dynamo_HeLaQC_500ng_2_EvoCol" mass_error_ppm="-0.2" retention_time="14.725379" start_time="14.5490789" end_time="15.131279" area="1.48347E+09" background="150119184" height="1.209676E+08" fwhm="0.202196673" truncated="false" identified="false" rank="1" points_across="34" user_set="TRUE" />
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            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_5" mass_error_ppm="4.3" retention_time="17.7742825" start_time="16.9462814" end_time="17.7782822" area="390351.5" background="0" height="73442.47" fwhm="0.08852874" fwhm_degenerate="true" truncated="false" identified="false" rank="8" points_across="3" rank_by_level="4" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_6_newIPA" retention_time="17.0262814" start_time="17.0262814" end_time="17.5222816" area="0" background="0" height="0" fwhm="0.496200562" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_7_newIPA" retention_time="16.4542789" start_time="16.4542789" end_time="16.99828" area="0" background="0" height="0" fwhm="0.5439606" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_8_newIPA" retention_time="15.9142771" start_time="15.9142771" end_time="16.4262772" area="0" background="0" height="0" fwhm="0.512084961" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_9_newIPA" retention_time="15.5422812" start_time="15.5422812" end_time="16.1582813" area="0" background="0" height="0" fwhm="0.615955353" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_10_newIPA" retention_time="15.0702763" start_time="15.0702763" end_time="15.626276" area="0" background="0" height="0" fwhm="0.5559597" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_12" retention_time="14.6903114" start_time="14.6903114" end_time="15.3023119" area="0" background="0" height="0" fwhm="0.611955643" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_13" retention_time="15.6782885" start_time="15.6782885" end_time="16.2022877" area="0" background="0" height="0" fwhm="0.523962" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_14" mass_error_ppm="10" retention_time="17.04188" start_time="16.47198" end_time="17.04598" area="151932.969" background="0" height="36966.0625" fwhm="0.06842008" fwhm_degenerate="true" truncated="false" identified="false" rank="8" points_across="2" rank_by_level="4" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_15" mass_error_ppm="0.2" retention_time="17.84228" start_time="17.26628" end_time="17.9902782" area="1378712.88" background="0" height="90379.3047" fwhm="0.283307582" fwhm_degenerate="true" truncated="false" identified="false" rank="8" points_across="3" rank_by_level="4" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_16_newIPA" mass_error_ppm="2" retention_time="17.4022827" start_time="17.3382835" end_time="17.8622837" area="1978498.38" background="0" height="183646.438" fwhm="0.1933766" truncated="false" identified="false" rank="7" points_across="2" rank_by_level="3" user_set="TRUE" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="6" calc_neutral_mass="488.186691" product_charge="1" cleavage_aa="E" loss_neutral_mass="0">
          <precursor_mz>644.822606</precursor_mz>
          <product_mz>489.193967</product_mz>
          <collision_energy>24.167614</collision_energy>
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            <transition_peak replicate="20210721_EvoHFX_HeLaQC_500ng_2" retention_time="14.4422474" start_time="14.4422474" end_time="14.757947" area="0" background="0" height="0" fwhm="0.315688133" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20210511_EvoCol_HEK_500ng_1" retention_time="16.319891" start_time="16.319891" end_time="16.8328915" area="0" background="0" height="0" fwhm="0.5131531" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="1" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20210511_EvoCol_HEK_500ng_2" retention_time="16.1686878" start_time="16.1686878" end_time="16.7896881" area="0" background="0" height="0" fwhm="0.620965958" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="1" user_set="TRUE" />
            <transition_peak replicate="20210511_EvoCol_HEK_1ug_1" retention_time="16.2766933" start_time="16.2766933" end_time="17.032692" area="0" background="0" height="0" fwhm="0.755958557" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="1" user_set="TRUE" />
            <transition_peak replicate="20210511_EvoCol_HEK_1ug_2" retention_time="15.8716936" start_time="15.8716936" end_time="16.4332943" area="0" background="0" height="0" fwhm="0.5615692" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="1" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211028_Dynamo_HeLaQC_500ng_2_EvoCol" retention_time="14.5490789" start_time="14.5490789" end_time="15.131279" area="0" background="0" height="0" fwhm="0.5821781" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="3" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_1" retention_time="15.3175793" start_time="15.3175793" end_time="15.92208" area="0" background="0" height="0" fwhm="0.604581833" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_2" retention_time="16.3422852" start_time="16.3422852" end_time="17.0662861" area="0" background="0" height="0" fwhm="0.7239475" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="3" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_3" retention_time="16.95428" start_time="16.95428" end_time="17.50628" area="0" background="0" height="0" fwhm="0.5522232" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_4" retention_time="16.9222851" start_time="16.9222851" end_time="17.5582848" area="0" background="0" height="0" fwhm="0.6359539" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="3" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_5" retention_time="16.9462814" start_time="16.9462814" end_time="17.7782822" area="0" background="0" height="0" fwhm="0.8319397" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="3" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_6_newIPA" retention_time="17.0262814" start_time="17.0262814" end_time="17.5222816" area="0" background="0" height="0" fwhm="0.496200562" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_7_newIPA" retention_time="16.4542789" start_time="16.4542789" end_time="16.99828" area="0" background="0" height="0" fwhm="0.5439606" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_8_newIPA" retention_time="15.9142771" start_time="15.9142771" end_time="16.4262772" area="0" background="0" height="0" fwhm="0.512084961" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_9_newIPA" retention_time="15.5422812" start_time="15.5422812" end_time="16.1582813" area="0" background="0" height="0" fwhm="0.615955353" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_10_newIPA" retention_time="15.0702763" start_time="15.0702763" end_time="15.626276" area="0" background="0" height="0" fwhm="0.5559597" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_12" retention_time="14.6903114" start_time="14.6903114" end_time="15.3023119" area="0" background="0" height="0" fwhm="0.611955643" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_13" retention_time="15.6782885" start_time="15.6782885" end_time="16.2022877" area="0" background="0" height="0" fwhm="0.523962" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_14" retention_time="16.47198" start_time="16.47198" end_time="17.04598" area="0" background="0" height="0" fwhm="0.574111938" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_15" retention_time="17.26628" start_time="17.26628" end_time="17.9902782" area="0" background="0" height="0" fwhm="0.7239475" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="3" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_16_newIPA" retention_time="17.3382835" start_time="17.3382835" end_time="17.8622837" area="0" background="0" height="0" fwhm="0.523962" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="TRUE" />
          </transition_results>
        </transition>
      </precursor>
    </peptide>
    <peptide standard_type="iRT" sequence="VEATFGVDESNAK" modified_sequence="VEATFGVDESNAK" calc_neutral_pep_mass="1365.641225" num_missed_cleavages="0" rt_calculator_score="34.779412320425337" predicted_retention_time="20.45" avg_measured_retention_time="19.7805634">
      <peptide_results>
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        <peptide_result replicate="20210721_EvoHFX_HeLaQC_500ng_2" peak_count_ratio="0.7" retention_time="17.5049477" predicted_retention_time="18.26" />
        <peptide_result replicate="20210511_EvoCol_HEK_500ng_1" peak_count_ratio="0.4" retention_time="20.0188923" predicted_retention_time="20.47" />
        <peptide_result replicate="20210511_EvoCol_HEK_500ng_2" peak_count_ratio="0.4" retention_time="20.0998878" predicted_retention_time="20.44" />
        <peptide_result replicate="20210511_EvoCol_HEK_1ug_1" peak_count_ratio="0.4" retention_time="19.954092" predicted_retention_time="20.29" />
        <peptide_result replicate="20210511_EvoCol_HEK_1ug_2" peak_count_ratio="0.4" retention_time="19.7380943" predicted_retention_time="20.23" />
        <peptide_result replicate="20211028_Dynamo_HeLaQC_500ng_2_EvoCol" peak_count_ratio="0.9" retention_time="18.3333778" predicted_retention_time="19.1" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_1" peak_count_ratio="0.8" retention_time="19.31898" predicted_retention_time="19.43" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_2" peak_count_ratio="1" retention_time="20.4582844" predicted_retention_time="21.03" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_3" peak_count_ratio="1" retention_time="20.79828" predicted_retention_time="21.4" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_4" peak_count_ratio="1" retention_time="20.8942852" predicted_retention_time="21.61" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_5" peak_count_ratio="0.8" retention_time="20.8502827" predicted_retention_time="21.69" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_6_newIPA" peak_count_ratio="1" retention_time="20.8342819" predicted_retention_time="21.64" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_7_newIPA" peak_count_ratio="0.9" retention_time="20.0662785" predicted_retention_time="20.91" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_8_newIPA" peak_count_ratio="0.9" retention_time="19.5222778" predicted_retention_time="20.28" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_9_newIPA" peak_count_ratio="0.9" retention_time="19.16628" predicted_retention_time="19.91" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_10_newIPA" peak_count_ratio="0.9" retention_time="18.6942768" predicted_retention_time="19.66" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_12" peak_count_ratio="0.8" retention_time="18.5583115" predicted_retention_time="19.22" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_13" peak_count_ratio="0.8" retention_time="19.5902882" predicted_retention_time="20.52" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_14" peak_count_ratio="1" retention_time="20.559679" predicted_retention_time="21.36" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_15" peak_count_ratio="1" retention_time="21.25828" predicted_retention_time="21.99" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_16_newIPA" peak_count_ratio="0.9" retention_time="21.2982826" predicted_retention_time="22.06" />
      </peptide_results>
      <precursor charge="2" calc_neutral_mass="1365.641225" precursor_mz="683.827888" auto_manage_children="false" collision_energy="25.571804" modified_sequence="VEATFGVDESNAK">
        <precursor_results>
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          <precursor_peak replicate="20210721_EvoHFX_HeLaQC_500ng_2" peak_count_ratio="0.7" retention_time="17.5049477" start_time="17.3860474" end_time="17.7427483" fwhm="0.104453251" area="1.89609431E+10" background="352605024" height="1.28244672E+09" mass_error_ppm="-0.07968414" truncated="0" identified="false" isotope_dotp="0.985087633" user_set="FALSE" />
          <precursor_peak replicate="20210511_EvoCol_HEK_500ng_1" peak_count_ratio="0.4" retention_time="20.0188923" start_time="19.8190918" end_time="20.2942924" fwhm="0.147118211" area="6.15414733E+09" background="111147912" height="303666624" mass_error_ppm="1.20978534" truncated="0" identified="false" isotope_dotp="0.982365668" user_set="FALSE" />
          <precursor_peak replicate="20210511_EvoCol_HEK_500ng_2" peak_count_ratio="0.4" retention_time="20.0998878" start_time="19.8352871" end_time="20.4670868" fwhm="0.164617151" area="1.166844E+10" background="170668816" height="498591360" mass_error_ppm="0.972846866" truncated="0" identified="false" isotope_dotp="0.980340362" user_set="FALSE" />
          <precursor_peak replicate="20210511_EvoCol_HEK_1ug_1" peak_count_ratio="0.4" retention_time="19.954092" start_time="19.6030922" end_time="20.3698921" fwhm="0.0809151456" area="9.238527E+09" background="0" height="8.195626E+08" mass_error_ppm="0.631347954" truncated="0" identified="false" isotope_dotp="0.9927102" user_set="TRUE" />
          <precursor_peak replicate="20210511_EvoCol_HEK_1ug_2" peak_count_ratio="0.4" retention_time="19.7380943" start_time="19.4788933" end_time="20.2888947" fwhm="0.1939899" area="4.73234842E+09" background="63535904" height="176728656" mass_error_ppm="1.13641346" truncated="0" identified="false" isotope_dotp="0.9878263" user_set="FALSE" />
          <precursor_peak replicate="20211028_Dynamo_HeLaQC_500ng_2_EvoCol" peak_count_ratio="0.9" retention_time="18.3333778" start_time="18.0217781" end_time="18.8417778" fwhm="0.2827149" area="1.01412628E+10" background="1.105188E+08" height="3.023413E+08" mass_error_ppm="0.6366951" truncated="0" identified="false" isotope_dotp="0.9902863" user_set="FALSE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_1" peak_count_ratio="0.8" retention_time="19.31898" start_time="18.96018" end_time="19.8376789" fwhm="0.219223171" area="1.63267871E+10" background="255218480" height="4.851284E+08" mass_error_ppm="-0.2545784" truncated="0" identified="false" isotope_dotp="0.976323" user_set="TRUE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_2" peak_count_ratio="1" retention_time="20.4582844" start_time="20.014286" end_time="21.1222858" fwhm="0.294742316" area="1.56672758E+10" background="250672752" height="444376576" mass_error_ppm="-0.145811111" truncated="0" identified="false" isotope_dotp="0.979060054" user_set="TRUE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_3" peak_count_ratio="1" retention_time="20.79828" start_time="20.5182819" end_time="21.08628" fwhm="0.201811537" area="1.451144E+10" background="6.879543E+08" height="4.890954E+08" mass_error_ppm="-0.03983733" truncated="0" identified="false" isotope_dotp="0.978650749" user_set="TRUE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_4" peak_count_ratio="1" retention_time="20.8942852" start_time="20.5382843" end_time="21.2502842" fwhm="0.280464053" area="1.55633961E+10" background="132204216" height="520186336" mass_error_ppm="-0.234312057" truncated="0" identified="false" isotope_dotp="0.977553" user_set="TRUE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_5" peak_count_ratio="0.8" retention_time="20.8502827" start_time="20.4782829" end_time="21.4262829" fwhm="0.269505858" area="2.32185569E+10" background="246713488" height="617090752" mass_error_ppm="-0.239229679" truncated="0" identified="false" isotope_dotp="0.9810571" user_set="TRUE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_6_newIPA" peak_count_ratio="1" retention_time="20.8342819" start_time="20.5142822" end_time="21.2262821" fwhm="0.2707616" area="1.53012828E+10" background="3.804123E+08" height="4.836384E+08" mass_error_ppm="-0.0116187874" truncated="0" identified="false" isotope_dotp="0.978730738" user_set="FALSE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_7_newIPA" peak_count_ratio="0.9" retention_time="20.0662785" start_time="19.7782784" end_time="20.374279" fwhm="0.263979733" area="1.533195E+10" background="498067168" height="528739360" mass_error_ppm="0.214899853" truncated="0" identified="false" isotope_dotp="0.9847817" user_set="TRUE" />
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            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_2" retention_time="23.1222858" start_time="23.1222858" end_time="23.8902855" area="0" background="0" height="0" fwhm="0.767944336" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="3" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_3" retention_time="23.69428" start_time="23.69428" end_time="24.29828" area="0" background="0" height="0" fwhm="0.604244232" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_4" retention_time="23.8502846" start_time="23.8502846" end_time="24.5782852" area="0" background="0" height="0" fwhm="0.727947235" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="3" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_5" retention_time="23.8942814" start_time="23.8942814" end_time="24.9022827" area="0" background="0" height="0" fwhm="1.00840759" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="3" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_6_newIPA" retention_time="23.8742828" start_time="23.8742828" end_time="24.5902824" area="0" background="0" height="0" fwhm="0.7159481" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_7_newIPA" retention_time="22.7782784" start_time="22.7782784" end_time="23.4022789" area="0" background="0" height="0" fwhm="0.6242523" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_8_newIPA" retention_time="21.914278" start_time="21.914278" end_time="22.5022774" area="0" background="0" height="0" fwhm="0.5879574" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_9_newIPA" retention_time="20.87428" start_time="20.87428" end_time="21.4502811" area="0" background="0" height="0" fwhm="0.575958252" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="1" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_10_newIPA" retention_time="20.9382763" start_time="20.9382763" end_time="21.4662762" area="0" background="0" height="0" fwhm="0.527961731" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_12" retention_time="20.8383121" start_time="20.8383121" end_time="21.4703121" area="0" background="0" height="0" fwhm="0.6319542" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="3" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_13" retention_time="22.4262886" start_time="22.4262886" end_time="23.3382874" area="0" background="0" height="0" fwhm="0.9119339" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="3" user_set="TRUE" />
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        </transition>
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    </peptide>
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        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_2" peak_count_ratio="0.7" retention_time="24.646286" predicted_retention_time="24.76" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_3" peak_count_ratio="0.8" retention_time="25.2582817" predicted_retention_time="25.15" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_4" peak_count_ratio="0.7" retention_time="25.4982853" predicted_retention_time="25.3" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_5" peak_count_ratio="0.6" retention_time="25.6102829" predicted_retention_time="25.41" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_6_newIPA" peak_count_ratio="0.6" retention_time="25.4782829" predicted_retention_time="25.31" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_7_newIPA" peak_count_ratio="0.5" retention_time="24.5662785" predicted_retention_time="24.56" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_8_newIPA" peak_count_ratio="0.6" retention_time="23.7782784" predicted_retention_time="23.9" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_9_newIPA" peak_count_ratio="0.6" retention_time="23.4662819" predicted_retention_time="23.54" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_10_newIPA" peak_count_ratio="0.6" retention_time="23.0982761" predicted_retention_time="23.17" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_12" peak_count_ratio="0.7" retention_time="22.93431" predicted_retention_time="22.98" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_13" peak_count_ratio="0.7" retention_time="24.3622875" predicted_retention_time="24.28" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_14" peak_count_ratio="0.6" retention_time="24.77038" predicted_retention_time="25.12" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_15" peak_count_ratio="0.7" retention_time="25.79428" predicted_retention_time="25.67" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_16_newIPA" peak_count_ratio="0.8" retention_time="25.9622822" predicted_retention_time="25.73" />
      </peptide_results>
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          <precursor_peak replicate="20210511_EvoCol_HEK_1ug_1" peak_count_ratio="0.4" retention_time="23.7232933" start_time="23.2858925" end_time="24.0796928" fwhm="0.08077936" area="5.5378903E+09" background="30409868" height="291387168" mass_error_ppm="1.24966" truncated="0" identified="false" isotope_dotp="0.986333549" user_set="TRUE" />
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          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_4" peak_count_ratio="0.7" retention_time="25.4982853" start_time="25.1502857" end_time="25.9302845" fwhm="0.6759139" area="7.068015E+09" background="170301168" height="235757248" mass_error_ppm="0.526281" truncated="0" identified="false" isotope_dotp="0.980169356" user_set="TRUE" />
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          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_10_newIPA" peak_count_ratio="0.6" retention_time="23.0982761" start_time="22.8462772" end_time="23.3622761" fwhm="0.3338586" area="6.48278374E+09" background="205798544" height="258583120" mass_error_ppm="0.4534629" truncated="0" identified="false" isotope_dotp="0.9897051" user_set="FALSE" />
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          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_15" peak_count_ratio="0.7" retention_time="25.79428" start_time="25.558279" end_time="26.1222782" fwhm="0.2755562" area="3.82833946E+09" background="348238336" height="125213888" mass_error_ppm="0.137495548" truncated="0" identified="false" isotope_dotp="0.976581633" user_set="TRUE" />
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          <product_mz>669.838059</product_mz>
          <collision_energy>25.06817</collision_energy>
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            <transition_peak replicate="20210511_EvoCol_HEK_500ng_2" mass_error_ppm="1.3" retention_time="23.9824867" start_time="23.7178879" end_time="24.2794876" area="2.81604454E+09" background="40900396" height="258949936" fwhm="0.170209318" truncated="false" identified="false" rank="1" points_across="58" user_set="FALSE" />
            <transition_peak replicate="20210511_EvoCol_HEK_1ug_1" mass_error_ppm="1" retention_time="23.7232933" start_time="23.2858925" end_time="24.0796928" area="2.56939341E+09" background="0" height="291387168" fwhm="0.08077936" truncated="false" identified="false" rank="1" points_across="68" user_set="TRUE" />
            <transition_peak replicate="20210511_EvoCol_HEK_1ug_2" mass_error_ppm="1.2" retention_time="23.7880936" start_time="23.5396938" end_time="24.128294" area="1.59619482E+09" background="2.495543E+07" height="151148784" fwhm="0.150545746" truncated="false" identified="false" rank="1" points_across="65" user_set="FALSE" />
            <transition_peak replicate="20211028_Dynamo_HeLaQC_500ng_2_EvoCol" mass_error_ppm="-0.1" retention_time="22.6711788" start_time="22.437479" end_time="23.0606785" area="1.88385792E+09" background="194775712" height="136691920" fwhm="0.2155701" truncated="false" identified="false" rank="1" points_across="34" user_set="FALSE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_1" mass_error_ppm="-0.1" retention_time="23.60508" start_time="23.39058" end_time="23.91318" area="2.58423322E+09" background="3.218832E+08" height="212545184" fwhm="0.187863365" truncated="false" identified="false" rank="1" points_across="28" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_2" mass_error_ppm="-0.4" retention_time="24.646286" start_time="24.3942852" end_time="25.1982861" area="3.054375E+09" background="138988384" height="209384384" fwhm="0.217351437" truncated="false" identified="false" rank="1" points_across="44" user_set="FALSE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_3" mass_error_ppm="0" retention_time="25.2582817" start_time="24.9902821" end_time="25.5542812" area="2.90641715E+09" background="85505544" height="201338016" fwhm="0.225737542" truncated="false" identified="false" rank="1" points_across="31" user_set="FALSE" />
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            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_10_newIPA" mass_error_ppm="-2" retention_time="23.1382771" start_time="22.8462772" end_time="23.3622761" area="2424338.75" background="0" height="135537.672" fwhm="0.351527244" fwhm_degenerate="true" truncated="false" identified="false" rank="6" points_across="2" rank_by_level="2" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_12" mass_error_ppm="-1.9" retention_time="22.9743118" start_time="22.7383118" end_time="23.43831" area="2571828" background="669416.9" height="126487.016" fwhm="0.306948453" truncated="false" identified="false" rank="7" points_across="2" rank_by_level="3" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_13" mass_error_ppm="-1.3" retention_time="24.4582882" start_time="23.8302879" end_time="24.9502888" area="1651733.5" background="0" height="81221.4453" fwhm="0.312438279" truncated="false" identified="false" rank="7" points_across="4" rank_by_level="3" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_14" mass_error_ppm="-2.2" retention_time="24.92618" start_time="24.55718" end_time="24.9302788" area="107951.75" background="0" height="15244.1436" fwhm="0.117979243" fwhm_degenerate="true" truncated="false" identified="false" rank="6" points_across="1" rank_by_level="2" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_15" mass_error_ppm="0.5" retention_time="25.8542786" start_time="25.558279" end_time="26.1222782" area="1688573.75" background="0" height="104109.32" fwhm="0.2755562" truncated="false" identified="false" rank="6" points_across="4" rank_by_level="2" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_16_newIPA" mass_error_ppm="-1.1" retention_time="25.9462833" start_time="25.6782837" end_time="26.1902828" area="3389219.25" background="0" height="218521.109" fwhm="0.258860141" truncated="false" identified="false" rank="8" points_across="2" rank_by_level="4" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="5" calc_neutral_mass="497.284949" product_charge="1" cleavage_aa="S" loss_neutral_mass="0">
          <precursor_mz>669.838059</precursor_mz>
          <product_mz>498.292225</product_mz>
          <collision_energy>25.06817</collision_energy>
          <transition_results>
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            <transition_peak replicate="20210721_EvoHFX_HeLaQC_500ng_2" mass_error_ppm="6" retention_time="21.0268478" start_time="20.9530468" end_time="21.3876476" area="753614.063" background="781407.5" height="43457.43" fwhm="0.348386765" fwhm_degenerate="true" truncated="false" identified="false" rank="7" points_across="2" rank_by_level="3" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20210511_EvoCol_HEK_500ng_1" retention_time="23.6854916" start_time="23.6854916" end_time="24.2794914" area="0" background="0" height="0" fwhm="0.593967438" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="1" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20210511_EvoCol_HEK_500ng_2" mass_error_ppm="-5.2" retention_time="24.2740879" start_time="23.7178879" end_time="24.2794876" area="179826.984" background="0" height="11784.4736" fwhm="0.254294932" fwhm_degenerate="true" truncated="false" identified="false" rank="5" points_across="1" rank_by_level="1" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20210511_EvoCol_HEK_1ug_1" retention_time="23.2858925" start_time="23.2858925" end_time="24.0796928" area="0" background="0" height="0" fwhm="0.7937565" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="4" user_set="TRUE" />
            <transition_peak replicate="20210511_EvoCol_HEK_1ug_2" retention_time="23.5396938" start_time="23.5396938" end_time="24.128294" area="0" background="0" height="0" fwhm="0.588567734" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="1" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211028_Dynamo_HeLaQC_500ng_2_EvoCol" retention_time="22.437479" start_time="22.437479" end_time="23.0606785" area="0" background="0" height="0" fwhm="0.6230316" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="4" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_1" retention_time="23.39058" start_time="23.39058" end_time="23.91318" area="0" background="0" height="0" fwhm="0.522670746" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_2" retention_time="24.3942852" start_time="24.3942852" end_time="25.1982861" area="0" background="0" height="0" fwhm="0.8039417" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="3" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_3" retention_time="24.9902821" start_time="24.9902821" end_time="25.5542812" area="0" background="0" height="0" fwhm="0.5639591" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="3" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_4" retention_time="25.1502857" start_time="25.1502857" end_time="25.9302845" area="0" background="0" height="0" fwhm="0.779943466" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="3" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_5" retention_time="25.346283" start_time="25.346283" end_time="26.1502819" area="0" background="0" height="0" fwhm="0.8039417" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="4" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_6_newIPA" retention_time="25.1662827" start_time="25.1662827" end_time="25.9142818" area="0" background="0" height="0" fwhm="0.7479458" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_7_newIPA" retention_time="24.3022785" start_time="24.3022785" end_time="24.76228" area="0" background="0" height="0" fwhm="0.45996666" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_8_newIPA" retention_time="23.5182781" start_time="23.5182781" end_time="24.1062775" area="0" background="0" height="0" fwhm="0.5879574" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="3" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_9_newIPA" retention_time="23.2062817" start_time="23.2062817" end_time="23.75028" area="0" background="0" height="0" fwhm="0.5439606" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_10_newIPA" retention_time="22.8462772" start_time="22.8462772" end_time="23.3622761" area="0" background="0" height="0" fwhm="0.5159626" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_12" retention_time="22.7383118" start_time="22.7383118" end_time="23.43831" area="0" background="0" height="0" fwhm="0.699949265" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_13" retention_time="23.8302879" start_time="23.8302879" end_time="24.9502888" area="0" background="0" height="0" fwhm="1.11991882" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="4" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_14" retention_time="24.55718" start_time="24.55718" end_time="24.9302788" area="0" background="0" height="0" fwhm="0.3729992" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="1" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_15" retention_time="25.558279" start_time="25.558279" end_time="26.1222782" area="0" background="0" height="0" fwhm="0.5639591" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="4" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_16_newIPA" mass_error_ppm="-2.5" retention_time="25.9462833" start_time="25.6782837" end_time="26.1902828" area="11044338" background="0" height="712087.7" fwhm="0.258860141" truncated="false" identified="false" rank="7" points_across="2" rank_by_level="3" user_set="FALSE" />
          </transition_results>
        </transition>
      </precursor>
    </peptide>
    <peptide standard_type="iRT" sequence="TPVITGAPYEYR" modified_sequence="TPVITGAPYEYR" calc_neutral_pep_mass="1365.692866" num_missed_cleavages="0" rt_calculator_score="51.038321249657514" predicted_retention_time="25.6" avg_measured_retention_time="25.243082">
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        <peptide_result replicate="20210511_EvoCol_HEK_500ng_1" peak_count_ratio="0.7" retention_time="24.9922924" predicted_retention_time="25.44" />
        <peptide_result replicate="20210511_EvoCol_HEK_500ng_2" peak_count_ratio="0.6" retention_time="25.0516872" predicted_retention_time="25.4" />
        <peptide_result replicate="20210511_EvoCol_HEK_1ug_1" peak_count_ratio="0.7" retention_time="24.7600937" predicted_retention_time="25.26" />
        <peptide_result replicate="20210511_EvoCol_HEK_1ug_2" peak_count_ratio="0.6" retention_time="24.8734932" predicted_retention_time="25.2" />
        <peptide_result replicate="20211028_Dynamo_HeLaQC_500ng_2_EvoCol" peak_count_ratio="0.6" retention_time="23.86838" predicted_retention_time="24.34" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_1" peak_count_ratio="0.7" retention_time="24.63858" predicted_retention_time="24.89" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_2" peak_count_ratio="0.7" retention_time="25.7982845" predicted_retention_time="26.28" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_3" peak_count_ratio="0.7" retention_time="26.5102825" predicted_retention_time="26.68" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_4" peak_count_ratio="0.7" retention_time="26.726284" predicted_retention_time="26.82" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_5" peak_count_ratio="0.7" retention_time="26.7382813" predicted_retention_time="26.94" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_6_newIPA" peak_count_ratio="0.6" retention_time="26.6382828" predicted_retention_time="26.82" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_7_newIPA" peak_count_ratio="0.7" retention_time="25.76228" predicted_retention_time="26.06" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_8_newIPA" peak_count_ratio="0.5" retention_time="24.9782772" predicted_retention_time="25.39" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_9_newIPA" peak_count_ratio="0.6" retention_time="24.61428" predicted_retention_time="25.03" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_10_newIPA" peak_count_ratio="0.7" retention_time="24.1982765" predicted_retention_time="24.61" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_12" peak_count_ratio="0.5" retention_time="24.038311" predicted_retention_time="24.52" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_13" peak_count_ratio="0.8" retention_time="25.5422878" predicted_retention_time="25.82" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_14" peak_count_ratio="0.8" retention_time="26.30378" predicted_retention_time="26.66" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_15" peak_count_ratio="0.7" retention_time="26.9022789" predicted_retention_time="27.18" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_16_newIPA" peak_count_ratio="0.6" retention_time="27.1622829" predicted_retention_time="27.23" />
      </peptide_results>
      <precursor charge="2" calc_neutral_mass="1365.692866" precursor_mz="683.853709" auto_manage_children="false" collision_energy="25.572734" modified_sequence="TPVITGAPYEYR">
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          <precursor_peak replicate="20210511_EvoCol_HEK_500ng_2" peak_count_ratio="0.6" retention_time="25.0516872" start_time="24.7762871" end_time="25.2730885" fwhm="0.16276364" area="6.51225E+09" background="7.061371E+07" height="386563456" mass_error_ppm="1.322786" truncated="0" identified="false" isotope_dotp="0.977574348" user_set="FALSE" />
          <precursor_peak replicate="20210511_EvoCol_HEK_1ug_1" peak_count_ratio="0.7" retention_time="24.7600937" start_time="24.5170937" end_time="25.0678921" fwhm="0.12774466" area="5.80481741E+09" background="168767984" height="4.56682E+08" mass_error_ppm="0.80375" truncated="0" identified="false" isotope_dotp="0.966875732" user_set="TRUE" />
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          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_4" peak_count_ratio="0.7" retention_time="26.726284" start_time="26.4342842" end_time="27.1422844" fwhm="0.21819903" area="6.4998656E+09" background="3.626912E+08" height="249417552" mass_error_ppm="0.6149592" truncated="0" identified="false" isotope_dotp="0.9502645" user_set="TRUE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_5" peak_count_ratio="0.7" retention_time="26.7382813" start_time="26.4982815" end_time="27.0982819" fwhm="0.226079315" area="7.5580544E+09" background="600133440" height="279821952" mass_error_ppm="0.414398164" truncated="0" identified="false" isotope_dotp="0.957297266" user_set="FALSE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_6_newIPA" peak_count_ratio="0.6" retention_time="26.6382828" start_time="26.3822823" end_time="26.8622818" fwhm="0.1888828" area="6.1704576E+09" background="1.350377E+08" height="272410016" mass_error_ppm="-0.339404225" truncated="0" identified="false" isotope_dotp="0.9789608" user_set="FALSE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_7_newIPA" peak_count_ratio="0.7" retention_time="25.76228" start_time="25.4302788" end_time="26.05028" fwhm="0.268074334" area="6.6120233E+09" background="100561560" height="2.772986E+08" mass_error_ppm="-0.253463864" truncated="0" identified="false" isotope_dotp="0.987040937" user_set="FALSE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_8_newIPA" peak_count_ratio="0.5" retention_time="24.9782772" start_time="24.6742783" end_time="25.2342777" fwhm="0.190785" area="7.62802944E+09" background="63585436" height="326485248" mass_error_ppm="-0.229548782" truncated="0" identified="false" isotope_dotp="0.9819656" user_set="FALSE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_9_newIPA" peak_count_ratio="0.6" retention_time="24.61428" start_time="24.3302822" end_time="24.8462811" fwhm="0.185795128" area="7.67361741E+09" background="179725536" height="3.508405E+08" mass_error_ppm="-0.237118021" truncated="0" identified="false" isotope_dotp="0.970603645" user_set="FALSE" />
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            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_14" retention_time="26.10698" start_time="26.10698" end_time="26.6522789" area="0" background="0" height="0" fwhm="0.545406342" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="TRUE" />
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            <transition_peak replicate="20210511_EvoCol_HEK_500ng_1" retention_time="24.7870922" start_time="24.7870922" end_time="25.2514915" area="0" background="0" height="0" fwhm="0.464374542" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="1" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20210511_EvoCol_HEK_500ng_2" retention_time="24.7762871" start_time="24.7762871" end_time="25.2730885" area="0" background="0" height="0" fwhm="0.496772766" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="1" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20210511_EvoCol_HEK_1ug_1" retention_time="24.5170937" start_time="24.5170937" end_time="25.0678921" area="0" background="0" height="0" fwhm="0.5507698" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="1" user_set="TRUE" />
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        </transition>
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          <product_mz>411.260196</product_mz>
          <collision_energy>25.572734</collision_energy>
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            <transition_peak replicate="20210511_EvoCol_HEK_1ug_2" retention_time="24.4090939" start_time="24.4090939" end_time="25.0570946" area="0" background="0" height="0" fwhm="0.6479645" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="1" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211028_Dynamo_HeLaQC_500ng_2_EvoCol" retention_time="23.5362778" start_time="23.5362778" end_time="24.2824783" area="0" background="0" height="0" fwhm="0.7463455" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_1" retention_time="24.29538" start_time="24.29538" end_time="25.04028" area="0" background="0" height="0" fwhm="0.745000839" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_2" retention_time="25.4742851" start_time="25.4742851" end_time="26.1102848" area="0" background="0" height="0" fwhm="0.6359539" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="TRUE" />
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            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_8_newIPA" retention_time="24.6742783" start_time="24.6742783" end_time="25.2342777" area="0" background="0" height="0" fwhm="0.5599594" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="FALSE" forced_integration="true" />
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          <precursor_mz>683.853709</precursor_mz>
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        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_10_newIPA" peak_count_ratio="0.9" retention_time="27.5182762" predicted_retention_time="29.51" />
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        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_16_newIPA" peak_count_ratio="0.6" retention_time="29.7502823" predicted_retention_time="32.34" />
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            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_15" mass_error_ppm="3.3" retention_time="33.69428" start_time="32.8422775" end_time="33.80628" area="4325014" background="0" height="330196.6" fwhm="0.241007566" fwhm_degenerate="true" truncated="false" identified="false" rank="8" points_across="4" rank_by_level="4" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_16_newIPA" retention_time="32.9622841" start_time="32.9622841" end_time="33.6302834" area="0" background="0" height="0" fwhm="0.667633057" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="3" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="5" calc_neutral_mass="483.232913" product_charge="1" cleavage_aa="P" loss_neutral_mass="0">
          <precursor_mz>726.835713</precursor_mz>
          <product_mz>484.240189</product_mz>
          <collision_energy>27.120086</collision_energy>
          <transition_results>
            <transition_peak replicate="20210721_EvoHFX_HeLaQC_500ng_1" retention_time="28.3311749" start_time="28.3311749" end_time="28.7165737" area="0" background="0" height="0" fwhm="0.385295868" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="1" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20210721_EvoHFX_HeLaQC_500ng_2" retention_time="27.9517479" start_time="27.9517479" end_time="28.4765472" area="0" background="0" height="0" fwhm="0.5246582" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="TRUE" />
            <transition_peak replicate="20210511_EvoCol_HEK_500ng_1" retention_time="30.8620911" start_time="30.8620911" end_time="31.574892" area="0" background="0" height="0" fwhm="0.7130127" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="1" user_set="TRUE" />
            <transition_peak replicate="20210511_EvoCol_HEK_500ng_2" retention_time="30.6784878" start_time="30.6784878" end_time="31.3102875" area="0" background="0" height="0" fwhm="0.631765366" fwhm_degenerate="true" truncated="false" identified="false" rank="0" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20210511_EvoCol_HEK_1ug_1" retention_time="30.6082935" start_time="30.6082935" end_time="31.2724934" area="0" background="0" height="0" fwhm="0.6641636" fwhm_degenerate="true" truncated="false" identified="false" rank="0" user_set="TRUE" />
            <transition_peak replicate="20210511_EvoCol_HEK_1ug_2" retention_time="30.6946945" start_time="30.6946945" end_time="31.348093" area="0" background="0" height="0" fwhm="0.6533642" fwhm_degenerate="true" truncated="false" identified="false" rank="0" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211028_Dynamo_HeLaQC_500ng_2_EvoCol" retention_time="30.178278" start_time="30.178278" end_time="31.58458" area="0" background="0" height="0" fwhm="1.40657425" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="6" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_1" retention_time="30.70308" start_time="30.70308" end_time="32.02518" area="0" background="0" height="0" fwhm="1.322279" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="5" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_2" retention_time="31.9822845" start_time="31.9822845" end_time="33.014286" area="0" background="0" height="0" fwhm="1.0324173" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="4" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_3" retention_time="32.346283" start_time="32.346283" end_time="33.3822823" area="0" background="0" height="0" fwhm="1.03543091" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="4" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_4" retention_time="31.9382839" start_time="31.9382839" end_time="33.0262833" area="0" background="0" height="0" fwhm="1.08844" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="4" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_5" retention_time="32.46228" start_time="32.46228" end_time="33.87028" area="0" background="0" height="0" fwhm="1.40856934" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="5" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_6_newIPA" retention_time="32.19828" start_time="32.19828" end_time="32.99428" area="0" background="0" height="0" fwhm="0.796321869" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="3" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_7_newIPA" retention_time="31.5382786" start_time="31.5382786" end_time="32.16628" area="0" background="0" height="0" fwhm="0.6279545" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_8_newIPA" retention_time="30.9742775" start_time="30.9742775" end_time="31.6262779" area="0" background="0" height="0" fwhm="0.651952744" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_9_newIPA" retention_time="30.5982819" start_time="30.5982819" end_time="31.386282" area="0" background="0" height="0" fwhm="0.7879429" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="3" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_10_newIPA" retention_time="30.1862755" start_time="30.1862755" end_time="30.8702755" area="0" background="0" height="0" fwhm="0.6842766" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="3" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_12" retention_time="29.9943123" start_time="29.9943123" end_time="30.2863121" area="0" background="0" height="0" fwhm="0.291978836" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="1" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_13" retention_time="31.4702873" start_time="31.4702873" end_time="32.7342873" area="0" background="0" height="0" fwhm="1.26451111" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="5" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_14" retention_time="33.54028" start_time="33.54028" end_time="33.99948" area="0" background="0" height="0" fwhm="0.459289551" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_15" retention_time="32.8422775" start_time="32.8422775" end_time="33.80628" area="0" background="0" height="0" fwhm="0.9643898" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="4" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_16_newIPA" retention_time="32.9622841" start_time="32.9622841" end_time="33.6302834" area="0" background="0" height="0" fwhm="0.667633057" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="3" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
      </precursor>
    </peptide>
    <peptide standard_type="iRT" sequence="GTFIIDPGGVIR" modified_sequence="GTFIIDPGGVIR" calc_neutral_pep_mass="1243.692472" num_missed_cleavages="0" rt_calculator_score="82.014015185955472" predicted_retention_time="35.4" avg_measured_retention_time="35.2805862">
      <peptide_results>
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        <peptide_result replicate="20210721_EvoHFX_HeLaQC_500ng_2" peak_count_ratio="0.8" retention_time="32.71185" predicted_retention_time="32.54" />
        <peptide_result replicate="20210511_EvoCol_HEK_500ng_1" peak_count_ratio="0.5" retention_time="34.550293" predicted_retention_time="34.91" />
        <peptide_result replicate="20210511_EvoCol_HEK_500ng_2" peak_count_ratio="0.5" retention_time="34.399086" predicted_retention_time="34.85" />
        <peptide_result replicate="20210511_EvoCol_HEK_1ug_1" peak_count_ratio="0.6" retention_time="34.2910919" predicted_retention_time="34.73" />
        <peptide_result replicate="20210511_EvoCol_HEK_1ug_2" peak_count_ratio="0.5" retention_time="34.1128922" predicted_retention_time="34.66" />
        <peptide_result replicate="20211028_Dynamo_HeLaQC_500ng_2_EvoCol" peak_count_ratio="0.9" retention_time="33.43778" predicted_retention_time="34.32" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_1" peak_count_ratio="0.8" retention_time="34.71618" predicted_retention_time="35.3" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_2" peak_count_ratio="0.8" retention_time="36.4622841" predicted_retention_time="36.29" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_3" peak_count_ratio="0.8" retention_time="36.87028" predicted_retention_time="36.75" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_4" peak_count_ratio="0.8" retention_time="36.8902855" predicted_retention_time="36.74" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_5" peak_count_ratio="0.8" retention_time="37.3822823" predicted_retention_time="36.94" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_6_newIPA" peak_count_ratio="0.9" retention_time="36.9982834" predicted_retention_time="36.68" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_7_newIPA" peak_count_ratio="0.9" retention_time="35.85028" predicted_retention_time="35.89" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_8_newIPA" peak_count_ratio="0.8" retention_time="34.8582764" predicted_retention_time="35.13" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_9_newIPA" peak_count_ratio="0.8" retention_time="34.5342827" predicted_retention_time="34.78" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_10_newIPA" peak_count_ratio="0.8" retention_time="33.57028" predicted_retention_time="34.05" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_12" peak_count_ratio="0.8" retention_time="33.87831" predicted_retention_time="34.63" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_13" peak_count_ratio="0.8" retention_time="35.7142868" predicted_retention_time="35.93" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_14" peak_count_ratio="0.8" retention_time="36.95968" predicted_retention_time="36.75" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_15" peak_count_ratio="0.7" retention_time="37.5502777" predicted_retention_time="37.05" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_16_newIPA" peak_count_ratio="0.9" retention_time="37.4622841" predicted_retention_time="37.08" />
      </peptide_results>
      <precursor charge="2" calc_neutral_mass="1243.692472" precursor_mz="622.853512" auto_manage_children="false" collision_energy="23.376726" modified_sequence="GTFIIDPGGVIR">
        <precursor_results>
          <precursor_peak replicate="20210721_EvoHFX_HeLaQC_500ng_1" peak_count_ratio="0.8" retention_time="32.972374" start_time="32.8165741" end_time="33.1117744" fwhm="0.0838120952" area="1.00309453E+10" background="6.039304E+08" height="8.986509E+08" mass_error_ppm="0.462134749" truncated="0" identified="false" isotope_dotp="0.9965056" user_set="FALSE" />
          <precursor_peak replicate="20210721_EvoHFX_HeLaQC_500ng_2" peak_count_ratio="0.8" retention_time="32.71185" start_time="32.47405" end_time="32.86765" fwhm="0.109900415" area="1.208483E+10" background="120740512" height="8.483736E+08" mass_error_ppm="0.336524427" truncated="0" identified="false" isotope_dotp="0.9929821" user_set="FALSE" />
          <precursor_peak replicate="20210511_EvoCol_HEK_500ng_1" peak_count_ratio="0.5" retention_time="34.550293" start_time="34.18309" end_time="34.89049" fwhm="0.0305291265" area="152637824" background="1092535.88" height="14857229" mass_error_ppm="-0.0811292753" truncated="0" identified="false" isotope_dotp="0.9943819" user_set="TRUE" />
          <precursor_peak replicate="20210511_EvoCol_HEK_500ng_2" peak_count_ratio="0.5" retention_time="34.399086" start_time="34.01029" end_time="34.62589" fwhm="0.186034217" area="2.694132E+09" background="29064586" height="116535752" mass_error_ppm="-0.400724173" truncated="0" identified="false" isotope_dotp="0.994837344" user_set="FALSE" />
          <precursor_peak replicate="20210511_EvoCol_HEK_1ug_1" peak_count_ratio="0.6" retention_time="34.2910919" start_time="33.95629" end_time="34.6582947" fwhm="0.231570348" area="9.657165E+09" background="156535936" height="329963936" mass_error_ppm="-0.300771683" truncated="0" identified="false" isotope_dotp="0.9912567" user_set="FALSE" />
          <precursor_peak replicate="20210511_EvoCol_HEK_1ug_2" peak_count_ratio="0.5" retention_time="34.1128922" start_time="33.907692" end_time="34.4476929" fwhm="0.186178878" area="147918064" background="0" height="7209333" mass_error_ppm="-0.3821433" truncated="0" identified="false" isotope_dotp="0.9595783" user_set="FALSE" />
          <precursor_peak replicate="20211028_Dynamo_HeLaQC_500ng_2_EvoCol" peak_count_ratio="0.9" retention_time="33.43778" start_time="33.0236778" end_time="34.3930779" fwhm="0.315070748" area="2.93138125E+09" background="1.12594E+07" height="6.761039E+07" mass_error_ppm="-0.3036011" truncated="0" identified="false" isotope_dotp="0.9738013" user_set="TRUE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_1" peak_count_ratio="0.8" retention_time="34.71618" start_time="34.32228" end_time="35.39868" fwhm="0.413885772" area="3.873503E+09" background="24877352" height="1.067128E+08" mass_error_ppm="1.29971886" truncated="0" identified="false" isotope_dotp="0.9855696" user_set="FALSE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_2" peak_count_ratio="0.8" retention_time="36.4622841" start_time="36.054287" end_time="37.1222839" fwhm="0.391222477" area="6.001159E+09" background="125364960" height="1.43482E+08" mass_error_ppm="0.8254496" truncated="0" identified="false" isotope_dotp="0.9808538" user_set="TRUE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_3" peak_count_ratio="0.8" retention_time="36.87028" start_time="36.3662834" end_time="37.48228" fwhm="0.3289442" area="6.02796646E+09" background="109469432" height="133635080" mass_error_ppm="1.09156919" truncated="0" identified="false" isotope_dotp="0.984329343" user_set="FALSE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_4" peak_count_ratio="0.8" retention_time="36.8902855" start_time="36.3222847" end_time="37.8502846" fwhm="0.343748331" area="7.621196E+09" background="8.020227E+07" height="153174992" mass_error_ppm="1.17239511" truncated="0" identified="false" isotope_dotp="0.9923051" user_set="FALSE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_5" peak_count_ratio="0.8" retention_time="37.3822823" start_time="36.9302826" end_time="38.09828" fwhm="0.3450174" area="3.375161E+09" background="34190256" height="8.023829E+07" mass_error_ppm="1.17001331" truncated="0" identified="false" isotope_dotp="0.98087734" user_set="TRUE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_6_newIPA" peak_count_ratio="0.9" retention_time="36.9982834" start_time="36.63428" end_time="37.3662834" fwhm="0.2843024" area="1.21540618E+10" background="219402944" height="344075936" mass_error_ppm="0.8169763" truncated="0" identified="false" isotope_dotp="0.9942595" user_set="FALSE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_7_newIPA" peak_count_ratio="0.9" retention_time="35.85028" start_time="35.47028" end_time="36.17428" fwhm="0.2716632" area="1.2147543E+10" background="206018736" height="385580960" mass_error_ppm="1.04605567" truncated="0" identified="false" isotope_dotp="0.99449867" user_set="FALSE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_8_newIPA" peak_count_ratio="0.8" retention_time="34.8582764" start_time="34.54228" end_time="35.25028" fwhm="0.2673062" area="1.37373891E+10" background="166481312" height="443485344" mass_error_ppm="0.7739275" truncated="0" identified="false" isotope_dotp="0.995472968" user_set="FALSE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_9_newIPA" peak_count_ratio="0.8" retention_time="34.5342827" start_time="34.20628" end_time="34.86628" fwhm="0.266002059" area="1.42513428E+10" background="254648976" height="4.687194E+08" mass_error_ppm="0.7384862" truncated="0" identified="false" isotope_dotp="0.993750036" user_set="FALSE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_10_newIPA" peak_count_ratio="0.8" retention_time="33.57028" start_time="33.218277" end_time="33.942276" fwhm="0.2845155" area="1.08472484E+10" background="7.233025E+07" height="341270848" mass_error_ppm="0.796914339" truncated="0" identified="false" isotope_dotp="0.994420052" user_set="FALSE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_12" peak_count_ratio="0.8" retention_time="33.87831" start_time="33.4303131" end_time="34.8903122" fwhm="0.480704546" area="2.345216E+09" background="4.634616E+07" height="4.625178E+07" mass_error_ppm="1.15333188" truncated="0" identified="false" isotope_dotp="0.9625771" user_set="TRUE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_13" peak_count_ratio="0.8" retention_time="35.7142868" start_time="35.1862869" end_time="36.41029" fwhm="0.392541468" area="1.49431053E+09" background="19501168" height="28618822" mass_error_ppm="1.30317783" truncated="0" identified="false" isotope_dotp="0.9391975" user_set="TRUE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_14" peak_count_ratio="0.8" retention_time="36.95968" start_time="36.4676781" end_time="37.82478" fwhm="0.352893025" area="2.2931E+09" background="29233366" height="50683536" mass_error_ppm="1.21440578" truncated="0" identified="false" isotope_dotp="0.982256651" user_set="TRUE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_15" peak_count_ratio="0.7" retention_time="37.5502777" start_time="37.2102776" end_time="38.2102776" fwhm="0.461925" area="1.75376E+09" background="145721888" height="40927892" mass_error_ppm="1.39589918" truncated="0" identified="false" isotope_dotp="0.9891667" user_set="TRUE" />
          <precursor_peak replicate="20211101_HeLaQC_500ng_EvoCol_16_newIPA" peak_count_ratio="0.9" retention_time="37.4622841" start_time="37.1102829" end_time="37.9422836" fwhm="0.287014455" area="1.53717289E+10" background="183178144" height="435681344" mass_error_ppm="0.7361903" truncated="0" identified="false" isotope_dotp="0.9934181" user_set="FALSE" />
        </precursor_results>
        <transition fragment_type="precursor" isotope_dist_rank="1" isotope_dist_proportion="0.482739925">
          <precursor_mz>622.853512</precursor_mz>
          <product_mz>622.853512</product_mz>
          <collision_energy>23.376726</collision_energy>
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            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_10_newIPA" mass_error_ppm="-2.5" retention_time="33.5142746" start_time="33.218277" end_time="33.942276" area="8.137854E+07" background="2318135.5" height="4471328" fwhm="0.282914817" truncated="false" identified="false" rank="7" points_across="3" rank_by_level="3" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_12" mass_error_ppm="-2.6" retention_time="33.8063126" start_time="33.4303131" end_time="34.8903122" area="14645798" background="1595795.5" height="548051.7" fwhm="0.435455978" truncated="false" identified="false" rank="7" points_across="5" rank_by_level="3" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_13" mass_error_ppm="1" retention_time="35.64229" start_time="35.1862869" end_time="36.41029" area="7195153" background="5006457" height="296553.375" fwhm="0.365483671" truncated="false" identified="false" rank="7" points_across="4" rank_by_level="3" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_14" mass_error_ppm="-3.4" retention_time="36.92278" start_time="36.4676781" end_time="37.82478" area="14789678" background="5172115.5" height="651874.5" fwhm="0.3421084" truncated="false" identified="false" rank="6" points_across="4" rank_by_level="2" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_15" mass_error_ppm="1" retention_time="37.51428" start_time="37.2102776" end_time="38.2102776" area="16279642" background="1608367.63" height="625973.5" fwhm="0.355741441" truncated="false" identified="false" rank="6" points_across="3" rank_by_level="2" user_set="TRUE" />
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        <transition fragment_type="b" fragment_ordinal="5" calc_neutral_mass="531.305684" product_charge="1" cleavage_aa="I" loss_neutral_mass="0">
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          <product_mz>532.31296</product_mz>
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            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_10_newIPA" mass_error_ppm="-0.8" retention_time="33.5142746" start_time="33.218277" end_time="33.942276" area="5159620.5" background="117019.859" height="290162.375" fwhm="0.278089345" truncated="false" identified="false" rank="8" points_across="3" rank_by_level="4" user_set="FALSE" forced_integration="true" />
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        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_3" peak_count_ratio="1" retention_time="41.55828" predicted_retention_time="40.56" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_4" peak_count_ratio="0.9" retention_time="41.6542854" predicted_retention_time="40.49" />
        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_5" peak_count_ratio="0.8" retention_time="41.8182831" predicted_retention_time="40.72" />
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    </peptide>
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        <peptide_result replicate="20211101_HeLaQC_500ng_EvoCol_8_newIPA" peak_count_ratio="0.9" retention_time="42.2542763" predicted_retention_time="40.79" />
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            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_12" retention_time="42.73431" start_time="42.73431" end_time="42.87431" area="0" background="0" height="0" fwhm="0.14005661" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="1" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_13" retention_time="42.7702866" start_time="42.7702866" end_time="42.9142876" area="0" background="0" height="0" fwhm="0.144058228" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="1" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_14" retention_time="42.76118" start_time="42.76118" end_time="42.90468" area="0" background="0" height="0" fwhm="0.14339447" fwhm_degenerate="true" truncated="false" identified="false" rank="0" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_15" mass_error_ppm="-8.1" retention_time="42.83428" start_time="42.80228" end_time="42.91028" area="5787375.5" background="0" height="2863111.5" fwhm="0.0339813232" fwhm_degenerate="true" truncated="false" identified="false" rank="8" points_across="1" rank_by_level="4" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_16_newIPA" retention_time="42.73828" start_time="42.73828" end_time="42.8262825" area="0" background="0" height="0" fwhm="0.08803558" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="1" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="8" calc_neutral_mass="904.480689" product_charge="1" cleavage_aa="Q" loss_neutral_mass="0">
          <precursor_mz>776.929751</precursor_mz>
          <product_mz>905.487965</product_mz>
          <collision_energy>28.923471</collision_energy>
          <transition_results>
            <transition_peak replicate="20210721_EvoHFX_HeLaQC_500ng_1" mass_error_ppm="3.6" retention_time="39.4339752" start_time="39.3109741" end_time="39.8316727" area="32632146" background="10631071" height="2249264.75" fwhm="0.2613579" truncated="false" identified="false" rank="6" points_across="2" rank_by_level="2" user_set="TRUE" />
            <transition_peak replicate="20210721_EvoHFX_HeLaQC_500ng_2" mass_error_ppm="0.3" retention_time="39.5096474" start_time="38.9930458" end_time="39.69005" area="4750211.5" background="0" height="317651.469" fwhm="0.259607852" truncated="false" identified="false" rank="9" points_across="2" rank_by_level="5" user_set="TRUE" />
            <transition_peak replicate="20210511_EvoCol_HEK_500ng_1" mass_error_ppm="-0.6" retention_time="41.926693" start_time="41.7214928" end_time="41.93209" area="383658" background="2365854.25" height="59192.2969" fwhm="0.107955933" fwhm_degenerate="true" truncated="false" identified="false" rank="5" rank_by_level="1" user_set="TRUE" />
            <transition_peak replicate="20210511_EvoCol_HEK_500ng_2" mass_error_ppm="-0.7" retention_time="41.7808876" start_time="41.7646866" end_time="42.034687" area="1138347.63" background="1839389.13" height="200252" fwhm="0.09497394" fwhm_degenerate="true" truncated="false" identified="false" rank="7" points_across="2" rank_by_level="3" user_set="TRUE" />
            <transition_peak replicate="20210511_EvoCol_HEK_1ug_1" mass_error_ppm="-0.6" retention_time="41.8348923" start_time="41.6296921" end_time="41.94829" area="5301173" background="7757985" height="410699.125" fwhm="0.262086272" fwhm_degenerate="true" truncated="false" identified="false" rank="5" points_across="1" rank_by_level="1" user_set="TRUE" />
            <transition_peak replicate="20210511_EvoCol_HEK_1ug_2" mass_error_ppm="-0.2" retention_time="41.7592926" start_time="41.5702934" end_time="41.8564949" area="951643.8" background="6569545.5" height="93315.06" fwhm="0.1917572" fwhm_degenerate="true" truncated="false" identified="false" rank="5" points_across="1" rank_by_level="1" user_set="TRUE" />
            <transition_peak replicate="20211028_Dynamo_HeLaQC_500ng_2_EvoCol" mass_error_ppm="6.2" retention_time="42.69558" start_time="42.6627769" end_time="42.7652779" area="27109538" background="65596676" height="11406611" fwhm="0.0390457" fwhm_degenerate="true" truncated="false" identified="false" rank="8" points_across="2" rank_by_level="4" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_1" mass_error_ppm="3.4" retention_time="42.75798" start_time="42.66828" end_time="42.92178" area="110460560" background="9.874308E+07" height="10797556" fwhm="0.172517776" fwhm_degenerate="true" truncated="false" identified="false" rank="6" points_across="1" rank_by_level="2" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_2" mass_error_ppm="0.2" retention_time="42.7582855" start_time="42.7302856" end_time="42.8342857" area="8874765" background="3.637566E+07" height="2263410.5" fwhm="0.06652984" fwhm_degenerate="true" truncated="false" identified="false" rank="7" points_across="1" rank_by_level="3" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_3" mass_error_ppm="4.4" retention_time="42.806282" start_time="42.7342834" end_time="42.94628" area="115100416" background="6.920876E+07" height="15405244" fwhm="0.130916327" truncated="false" identified="false" rank="7" points_across="1" rank_by_level="3" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_4" mass_error_ppm="4.1" retention_time="42.7942848" start_time="42.7542839" end_time="42.858284" area="3.488944E+07" background="61754456" height="8445831" fwhm="0.08427625" truncated="false" identified="false" rank="7" points_across="1" rank_by_level="3" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_5" mass_error_ppm="4.6" retention_time="42.7822838" start_time="42.75828" end_time="42.83028" area="3422977.25" background="24003482" height="1259561" fwhm="0.04828514" fwhm_degenerate="true" truncated="false" identified="false" rank="7" points_across="1" rank_by_level="3" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_6_newIPA" mass_error_ppm="3.9" retention_time="42.714283" start_time="42.6742821" end_time="42.7822838" area="2483554" background="0" height="2810526.75" fwhm="0.0146582685" truncated="false" identified="false" rank="6" points_across="2" rank_by_level="2" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_7_newIPA" retention_time="42.36628" start_time="42.36628" end_time="42.71428" area="0" background="0" height="0" fwhm="0.347808838" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="2" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_8_newIPA" mass_error_ppm="9.1" retention_time="42.4262772" start_time="42.0182762" end_time="42.4822769" area="661510.563" background="0" height="68664.91" fwhm="0.160561" truncated="false" identified="false" rank="7" points_across="2" rank_by_level="3" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_9_newIPA" mass_error_ppm="3.1" retention_time="41.9502831" start_time="41.71828" end_time="42.25828" area="61862004" background="0" height="4633110.5" fwhm="0.221706808" truncated="false" identified="false" rank="7" points_across="3" rank_by_level="3" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_10_newIPA" mass_error_ppm="5.4" retention_time="41.3942757" start_time="41.1382751" end_time="41.6102753" area="6.869666E+07" background="3824143" height="4533598.5" fwhm="0.254876465" truncated="false" identified="false" rank="6" points_across="1" rank_by_level="2" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_12" mass_error_ppm="1.2" retention_time="42.7703133" start_time="42.73431" end_time="42.87431" area="2.031755E+07" background="48145604" height="3835924" fwhm="0.08912196" fwhm_degenerate="true" truncated="false" identified="false" rank="7" points_across="1" rank_by_level="3" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_13" mass_error_ppm="3.2" retention_time="42.8102875" start_time="42.7702866" end_time="42.9142876" area="3043359.25" background="7283203" height="559091.4" fwhm="0.0920372" fwhm_degenerate="true" truncated="false" identified="false" rank="7" points_across="1" rank_by_level="3" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_14" mass_error_ppm="6.3" retention_time="42.90058" start_time="42.76118" end_time="42.90468" area="9072.799" background="165072.891" height="2048.838" fwhm="0.07374573" fwhm_degenerate="true" truncated="false" identified="false" rank="6" rank_by_level="2" user_set="TRUE" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_15" mass_error_ppm="0.8" retention_time="42.83428" start_time="42.80228" end_time="42.91028" area="11543146" background="0" height="5710587.5" fwhm="0.0339813232" fwhm_degenerate="true" truncated="false" identified="false" rank="6" points_across="1" rank_by_level="2" user_set="FALSE" forced_integration="true" />
            <transition_peak replicate="20211101_HeLaQC_500ng_EvoCol_16_newIPA" mass_error_ppm="-1.9" retention_time="42.7742844" start_time="42.73828" end_time="42.8262825" area="14774018" background="19410262" height="4529914.5" fwhm="0.0620915033" fwhm_degenerate="true" truncated="false" identified="false" rank="8" points_across="1" rank_by_level="4" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
      </precursor>
    </peptide>
  </peptide_list>
</srm_settings>